An early-screening biomarker of endometrial carcinoma: NGAL is associated with epithelio-mesenchymal transition

Abstract

neutrophilgelatinase-associated lipocalin is currently one of the most interesting and enigmatic proteins involved in the development of malignancies. In this study, we found that the expression of neutrophilgelatinase-associated lipocalin was up-regulated in endometrial cancer tissues and cell lines, significantly increased in early-grade ones, suggesting it may serve as a biomarker for early-stage screening for endometrial carcinoma. Moreover, neutrophilgelatinase-associated lipocalin was up-regulated in Ishikawa cells under going epithelio-mesenchymal transition induced by epidermal growth factor (5 ng/ml). Up-regulation of neutrophilgelatinase-associated lipocalin may correlate with the down-regulation of E-cadherin expression, up-regulation of Vimentin expression, enhanced cell migration, invasion and proliferation, which are the typical hallmarks of epithelio-mesenchymal transition processes. neutrophilgelatinase-associated lipocalin may play a dual role during tumorigenetic and developmental processes of endometrial carcinoma. These results suggested neutrophilgelatinase-associated lipocalin to be a potential molecular target in the early diagnosis and treatment of endometrial carcinoma. Further studies are warranted to clarify the molecular mechanisms behind the expression and function of neutrophilgelatinase-associated lipocalin and epithelio-mesenchymal transition.

MicroRNA-761 suppresses remodeling of nasal mucosa and epithelial–mesenchymal transition in mice with chronic rhinosinusitis through LCN2

“…In epithelial cells from nasal polyps in CRS, an opposite trend of E-cadherin and N-cadherin expression was identified [20], which was consistent with our finding that miR-761 downregulated N-cadherin but upregulated E-cadherin. In addition, a recent report showcased that the downregulation of E-cadherin and upregulation of vimentin were related to the elevation of neutrophil gelatinase-associated lipocalin [30], which was in line with our results that the depletion of LCN2 led to an increase in E-cadherin and reduced vimentin. LCN2 has been previously shown to reversely regulate EMT via the LCN2/Twist signaling pathway in hepatocellular carcinoma [18].…”

Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies

“…NGAL, a NET-associated protein, is known to promote EMT. 129 The mechanisms involved in the development of subtarsal fibrosis in ocular GVHD have not fully been defined. Because transforming growth factor beta (TGF-β) is known to cause fibrosis, we analyzed the levels TGF-β in ocular surface washings and NETs.…”

Analytical validation of Gentian NGAL particle-enhanced enhanced turbidimetric immunoassay (PETIA)

“…The clinical significance of measuring NGAL has also been recently highlighted in conditions other then AKI, such as acute bacterial meningitis [10] and bacterial peritonitis [11] . Notably, a growing number of studies have reported that NGAL may be used as an efficient biomarker for early diagnosis and predicting the clinical outcome of certain types of cancer [12] , [13] , [14] .…”