An Easy Efficient Method of Veterinary Drug Residue Analysis in Raw Milk by RP-HPLC-UV with Application to Raw Milk

Abstract

Background: This study reports an easy method of veterinary drug investigation in raw milk, based on QuECHERS extraction followed by RP-HPLC-UV analysis. Use of this benchtop system was motivated by its availability and moderate cost relatively to other sophisticated methods such as LC-MS which are more performant. Methods: This developed method has been optimized and then after validated according to EU legislation, demonstrating good linearity with R²>0.997, acceptable peak resolution within short time (˂9.5 min) and a good recovery of analyzed drugs (OXY, ALZ and IVR, respectively 87.08, 99.02 and 92.01%). Additionally we applied the method to analysis of cow milk, collected in Nouakchott, capital of Mauritania. Results: Obtained results indicates a mixed level of drug use according to targeted molecules. In 42% of sampled farms the anti-parasitics IVR and ALZ were detected whereas the antibiotic OXY was detected in 50%. Conclusion: This investigation shows that 17% of sampled farms exceed European standards for IVR drug.

A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications

“…In conclusion, RP-HPLC is an essential technique in pharmaceutical analysis due to its high sensitivity, selectivity, and reproducibility. The applications of RP-HPLC in pharmaceutical analysis include drug purity determination, impurity analysis, analysis of drug formulations, analysis of drug degradation products, analysis of drug-drug interactions, drug stability studies, and determination of bioavailability and pharmacokinetics of drugs [43].…”