An Effect of Inoculum Quantity of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) on the Nematode Load of Monochamus alternatus (Coleoptera: Cerambycidae) in Laboratory

Aikawa, Takuya; Togashi, Katsumi

doi:10.3725/jjn1993.27.1_14

Cited by 11 publications

(3 citation statements)

References 8 publications

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“…D3 nematodes were collected from two-month old cultures (1/5 PDA supplemented with 3% glycerol). Nematodes of D4 stage were produced and isolated using the artificial pupal chamber method 18,80 . Nematodes were frozen in 150 µL TRI reagent (Life Technology) in liquid nitrogen and homogenised using Biomasher (TaKaRa).…”

Section: Methodsmentioning

confidence: 99%

Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle

Tanaka

Dayı

Maeda

et al. 2019

Sci Rep

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The pine wood nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease, one of the most devastating forest diseases in East Asian and West European countries. The lifecycle of B. xylophilus includes four propagative larval stages and gonochoristic adults which are involved in the pathogenicity, and two stages of dispersal larvae involved in the spread of the disease. To elucidate the ecological roles of each developmental stage in the pathogenic life cycle, we performed a comprehensive transcriptome analysis using RNA-seq generated from all developmental stages of B. xylophilus and compared transcriptomes between stages. We found more than 9000 genes are differentially expressed in at least one stage of the life cycle including genes involved in general nematode biology such as reproduction and moulting but also effector genes likely to be involved in parasitism. The dispersal-stage transcriptome revealed its analogy to C. elegans dauer and the distinct roles of the two larval stages from each other regarding survival and transmission. This study provides important insights and resources to understand B. xylophilus parasitic biology.

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Section: Methodsmentioning

confidence: 99%

Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle

Tanaka

Dayı

Maeda

et al. 2019

Sci Rep

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“…An artificial pupal chamber was prepared following the method by Aikawa and Togashi . A hole (3–4 cm deep and 1 cm in diameter) was drilled at the center of a cut end of healthy P.…”

Section: Methodsmentioning

confidence: 99%

“…An artificial pupal chamber was prepared following the method by Aikawa and Togashi. 31 A hole (3−4 cm deep and 1 cm in diameter) was drilled at the center of a cut end of healthy P. thunbergii (5 cm height and 2.5 cm in diameter). The logs were autoclaved at 121 °C for 15 min and then incubated at 25 °C in the dark for 14 days after inoculation with B. cinerea as a food source for B. xylophilus.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Rapid Diagnosis of the Infection of Pine Tree with Pine Wood Nematode (Bursaphelenchus xylophilus) by Use of Host-Tree Volatiles

Yun

Kim

Park

2012

J. Agric. Food Chem.

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Attraction of the Bursaphelenchus xylophilus nematode toward 18 volatiles of Pinus species was evaluated by a Petri-dish bioassay under laboratory conditions to develop a rapid diagnostic kit. Among these compounds, α-pinene, β-pinene, and camphor showed significantly higher attractiveness to B. xylophilus in both the reproductive and dispersal stages, whereas these compounds were not active against Bursaphelenchus mucronatus. A trap tube was developed as a diagnostic kit, which consisted of a tube filled with 0.8% agar and a matrix impregnated with an attractant: α-pinene, β-pinene, or camphor. All tested compounds attracted a significantly higher number of B. xylophilus than that in the control treatment. No significant difference was observed among attractants. The cotton-ball matrix was significantly more effective than the filter-paper matrix for attracting B. xylophilus in the artificial pupal chamber bioassay. In a bioassay with pine wood nematode (PWN)-infected pine tree logs, B. xylophilus was initially attracted after an 8 h trap period and the number of B. xylophilus increased with time. The trap tube using camphor and the cotton-ball matrix were most effective for attracting B. xylophilus. The semiochemical-based tube-trapping method is simple to use, requires minimal labor, and is economical and effective for detecting B. xylophilus living in host pine trees during field sampling.

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Chemotaxis of the Pinewood Nematode, Bursaphelenchus xylophilus, to Volatiles Associated with Host Pine, Pinus massoniana, and its Vector Monochamus alternatus

et al. 2007

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The pinewood nematode (PWN), Bursaphelenchus xylophilus, the most important invasive species in pine forests of Asia, is transported to new pine hosts by beetles of the genus Monochamus. Third-stage dispersal juveniles (J(III)) aggregate in pupal chambers around the vector as it matures. We demonstrated that the ratio of three terpenes (alpha-pinene, beta-pinene, and longifolene at 1:2.7:1.1) released by larval Monochamus alternatus strongly attract J(III), whereas the different ratio (1:0.1:0.01) of these three terpenes found in healthy xylem of Pinus massoniana attracts only the propagative stage (J(n)) of the nematode. We suggest that the volatiles produced by the host plants could be the basis of a chemoecological relationship between plant parasitic nematodes and their vector insects. Capture of J(III) with terpene-baited trap tubes deployed for 2 hr in the field was demonstrated. This technique may lead to the development of rapid sampling methodologies for use at either ports-of-entry or in the field.

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An Effect of Inoculum Quantity of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) on the Nematode Load of Monochamus alternatus (Coleoptera: Cerambycidae) in Laboratory

Cited by 11 publications

References 8 publications

Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle

Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle

Rapid Diagnosis of the Infection of Pine Tree with Pine Wood Nematode (Bursaphelenchus xylophilus) by Use of Host-Tree Volatiles

Chemotaxis of the Pinewood Nematode, Bursaphelenchus xylophilus, to Volatiles Associated with Host Pine, Pinus massoniana, and its Vector Monochamus alternatus

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