An effective method for deproteinization of bioactive polysaccharides extracted from lingzhi (Ganoderma atrum)

Chen, Yi; Xie, Mingyong; Li, Wenjuan; Zhang, Hui; Nie, Shaoping; Wang, Yuanxing; Li, Chang

doi:10.1007/s10068-012-0024-2

Cited by 49 publications

(18 citation statements)

References 33 publications

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“…Determination of the total content of polysaccharides: The reference method was the improved phenol-sulphuric acid method, which was performed as described by Chen, Xie, Li, et al (2012).…”

Section: Chemical Analysismentioning

confidence: 99%

Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics

et al. 2012

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“…Determination of the total content of polysaccharides: The reference method was the improved phenol-sulphuric acid method, which was performed as described by Chen, Xie, Li, et al (2012).…”

Section: Chemical Analysismentioning

confidence: 99%

Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics

et al. 2012

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“…Among them, the Bradford method is faster, involves fewer mixing steps, does not require heating, and gives a more stable colorimetric response than other assays. Because of those advantages, the Bradford assay using bovine serum albumin (BSA) as standard was used for determining protein content in MA polysaccharides . Results were obtained from averaging at least three experiments for MA sample.…”

Section: Resultsmentioning

confidence: 99%

Development of dense films from Melia azedarach polysaccharides

Masuelli

Garcı́a

Marchese

et al. 2014

J of Applied Polymer Sci

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The aim of this work was to prepare dense films from polysaccharides extracted from Melia azedarach (MA) to be used e.g. in agricultural industry. Crosslinking of MA films with glutaraldehyde (Glu) through immersion method were performed. Structural features of films were elucidated by FTIR and XRD analysis. The influence of crosslinking in mechanical properties, water uptake and water vapor permeation was evaluated. Results showed neat Melia azedarach film presented high elongation and elastic modulus. Hydroxyl functional groups present in MA were reacted with glutaraldheyde to render a crosslinked matrix. It was observed through FTIR analysis that OH band reduces intensity due to the formation of acetal linkage, as the crosslinking time increases. XRD evidenced structural changes in MA arrangement with the crosslinking time. Chemical crosslinking during 12 h and 24 h rendered insoluble but less deformable films. Water uptake and water vapor permeability were reduced as the crosslinking time increased. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41624.

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“…This method has, however, been questioned, not only because of the formation of protein-EPS complexes [56]. Chen et al [57] compared the Sevag method with enzymatic protein cleavage through papain and subsequent lead acetate precipitation, and claimed that this procedure resulted in highest purification rates and lowest EPS losses. Protein precipitation with trichloroacetic acid (TCA), used for purification of bacterial EPS [58,59], was less frequently applied for fungal EPS purification.…”

Section: Isolation and Quantification Of Fungal Exopolysaccharidesmentioning

confidence: 99%

Exopolysaccharides from Basidiomycota: Formation, isolation and techno‐functional properties

Jaros

Köbsch

Rohm

2018

Engineering in Life Sciences

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This Mini Review gives an overview of and respective references for the production and properties of exopolysaccharides from Basidiomycota in submerged cultivation. Media and conditions that are usually applied in laboratory culture are summarized, and the lack of studies related to up‐scaling is addressed. Procedures for isolation and purification of the exopolysaccharides from the fermentation media are reviewed, and challenges related to exopolysaccharide quantification are discussed. Finally, the techno‐functional properties of the respective exopolysaccharides, and potential applications in foods are addressed.

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An effective method for deproteinization of bioactive polysaccharides extracted from lingzhi (Ganoderma atrum)

Cited by 49 publications

References 33 publications

Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics

Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics

Development of dense films from Melia azedarach polysaccharides

Exopolysaccharides from Basidiomycota: Formation, isolation and techno‐functional properties

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