An efficient droplet-vitrification cryopreservation for valuable blueberry germplasm

Wang, Li-Yu; Li, Yadong; Sun, Haiyue; Liu, Haiguang; XueDong, Tang; Wang, Qiao‐Chun; Zhang, Zhidong

doi:10.1016/j.scienta.2017.03.007

Cited by 28 publications

(12 citation statements)

References 41 publications

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“…Working on Argyranthemum, Zhang et al (2015) reported that root formation and plant growth were reduced in the cryo-derived plants grown in the greenhouse, produces more dependable results than those using only one marker (Wang et al 2014a(Wang et al , b, 2018. ISSR and AFLP did not detect any polymorphic bands in Solanum tuberosum plants cryopreserved by droplet-vitrification, encapsulation-vitrification and vitrification (Wang et al 2014b;Li et al 2017 (Ahuja et al 2002). Similar levels of five major biochemical compounds were found in the flowers harvested from the cryopreserved plants and the control in Chrysanthemum morifolium 'Hangju' (Bi et al 2016).…”

Section: Discussionmentioning

confidence: 73%

“…However, such studies have been quite limited in cryo-derived regenerants of Allium spp. (Liu et al 2017). Evaluations of field performance in cryo-derived plants provided valuable information for use of cryopreservation for establishment of cryo-banks (Salama et al 2018).…”

mentioning

confidence: 99%

“…In the genus Allium, cryopreservation has been well-established for garlic (Keller 2002(Keller , 2005Keller et al 2011;Ellis et al 2006;Kim et al 2006Kim et al , 2007Liu et al 2017), while quite a few addressed shallot (Kim et al 2007). We previously reported a droplet-vitrification for efficient cryopreservation of shallot shoot tips (Wang et al 2019(Wang et al , 2020.…”

mentioning

confidence: 99%

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Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Wang

Hamborg

Elameen

et al. 2020

Plant Cell Tiss Organ Cult

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Section: Discussionmentioning

confidence: 73%

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confidence: 99%

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confidence: 99%

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Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Wang

Hamborg

Elameen

et al. 2020

Plant Cell Tiss Organ Cult

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“…Blueberry is a perennial evergreen or deciduous shrub that is native to North America (Wang et al, 2017). Northern highbush blueberry (Vaccinium corymbosum), rabbiteye blueberry, and southern highbush blueberry (V. corymbosum interspecific hybrids) are the three most commercially important species cultivated (Yu et al, 2016).…”

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confidence: 99%

High Temperatures during Flowering Reduce Fruit Set in Rabbiteye Blueberry

Yang¹,

Er²,

Fu³

et al. 2019

J. Amer. Soc. Hort. Sci.

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After nearly a decade of development, the scale of blueberry (Vaccinium sp.) cultivation has increased, particularly in south China; however, this region is becoming increasingly challenged by temperature changes during the flowering phenophase. Understanding the effects of temperature on pollen germination and pollen tube growth in blueberry is thus important. Using the rabbiteye blueberry (V. ashei) ‘Brightwell’, different temperature treatments were carried out during open pollination and cross-pollination with the pollen from rabbiteye blueberry ‘Gardenblue’ in field, greenhouse, and controlled temperature experiments over two consecutive years. The differences in pollen germination, pollen tube dynamics, and ovule viability following different treatments were analyzed, and the critical temperatures were calculated using quadratic and modified bilinear equations to quantify the developmental responses to temperature. The results showed that the fruit set of the artificially pollinated plants inside the greenhouse was significantly higher than that outside the greenhouse. Furthermore, pollen germination and pollen tube growth gradually accelerated under the appropriate high-temperature range, resulting in reduced pollen tube travel time to the ovule. However, the percentage of the style traversed by the pollen tube did not increase at temperatures greater than 30 °C, and a high-temperature range could accelerate ovule degeneration. Therefore, impairment of pollen tube growth in the upper half of the style following pollen germination and ovule degeneration constituted important factors leading to reduced fruit setting under short periods of high temperature during the flowering phenophase in rabbiteye blueberry. This work advances our understanding of the effect of temperature on pollen germination, pollen tube growth, ovule longevity, and fruit setting in rabbiteye blueberry, and provides a foundation for continued cultivation and breeding enhancement. The findings propose that the tolerance of rabbiteye blueberry to a certain high-temperature range in the flowering phenophase should inform breeding strategies for temperature resistance and that temperature range is also an important indicator of suitable environments for cultivation to mitigate potential temperature stress.

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“…Similar results were obtained in vegetable crops, such as Phaseolus vulgariss (seeds) by SSR [ 97 ], Asparagus officinalis (rhizome buds) by EST-SSR and FCM [ 98 ], and Allium by SSR [ 81 ], AFLP and ISSR [ 99 ]. Examples of analyses of other crops included fruit trees, such as Musa (suck meristem) by SSR [ 100 ], Malus by FCM and ISSR [ 101 , 102 ], Vaccinium corymbosum by RAPD and ISSR [ 103 , 104 ], Vitis by RAPD and ISSR [ 17 ], and Actinidia chinensis by AFLP and ISSR [ 71 ], and medicinal species, such as Rabdosia rubescens by FCM and SRAP [ 105 ] and Bacopa monnieri by RAPD [ 106 ].…”

Section: Assessments Of Epigenetic and Genetic Integritymentioning

confidence: 99%

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Shukla

Ren

et al. 2021

Plants

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Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

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An efficient droplet-vitrification cryopreservation for valuable blueberry germplasm

Cited by 28 publications

References 41 publications

Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

High Temperatures during Flowering Reduce Fruit Set in Rabbiteye Blueberry

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

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