An Efficient In vitro Hardening Technique of Tissue Culture Raised Plants

Deb, Chitta Ranjan; Imchen, Temjensangba

doi:10.3923/biotech.2010.79.83

Cited by 58 publications

(29 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it is estimated as the main limitation using this technology on commercial scale (Chandra et al 2010;Deb and Imchen 2010). During in vitro cultivation, plantlets grow in ambient temperature (25 ± 2 °C) under low light intensity, hence direct transfer to broad spectrum sunlight and ex vitro temperature (26-36 °C) might result in their quick wilting and dying (Lavanya et al 2009;Matysiak and Gabryszewska 2016).…”

Section: Discussionmentioning

confidence: 99%

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

Muszyńska

Hanus-Fajerska

2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

Muszyńska

Hanus-Fajerska

2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

“…Microshoots on being transferred to ex vitro conditions are exposed to abiotic (altered temperature, light intensity and humidity conditions) and biotic stress conditions i.e. soil microflora, so need acclimatization for successful establishment and survival of plantlets (Deb and Imchen 2010). In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, research has been focused on the control of the environmental conditions (both physical and chemical) and to acclimatize the plants to compete with soil microflora (Mathur et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Acclimatization of tissue cultured plantlets: from laboratory to land

et al. 2010

View full text Add to dashboard Cite

The ultimate success of micropropagation on a commercial scale depends on the ability to transfer plants out of culture on a large scale, at low cost and with high survival rates. During field transfer the in vitro grown plantlets are unable to compete with soil microbes and to cope with the environmental conditions. The in vitro culture conditions result in the plantlets with altered morphology, anatomy and physiology. In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, efforts are focused on the control of both physical and chemical environment and biohardening of micropropagated plantlets. This review describes the abiotic and biotic stresses and current developing methods for the acclimatization of microshoots.

show abstract

“…In the last two decades, several studies regarding bromeliads have been published (Silveira et al, 2009;Santa-Rosa et al, 2013). After in vitro propagation, different systems are used in plantlet acclimatization (Deb and Imchen, 2010), a critical micropropagation phase which represents a limiting factor for its success (Chandra et al, 2010). Plant survival in field conditions depends on the quality of in vitro cultures (Faisal and Anis, 2009).…”

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

View full text Add to dashboard Cite

This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

show abstract

An Efficient In vitro Hardening Technique of Tissue Culture Raised Plants

Cited by 58 publications

References 8 publications

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

Acclimatization of tissue cultured plantlets: from laboratory to land

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Contact Info

Product

Resources

About