In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

Muszyńska, Ewa; Hanus-Fajerska, Ewa

doi:10.1007/s11240-016-1140-0

Cited by 19 publications

(13 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present work, the tissue culture system competent to regenerate large amount of uniform plant material ready to be applied on heavy metal-polluted areas was elaborated. To our knowledge, it is the first report on in vitro propagation of G. fastigiata calamine ecotype and one of the few reports relating to the use of these techniques for multiplication of facultative metallophytes (Bidwell et al 2001 ; Jack et al 2005 ; Zheng et al 2007 ; Muszyńska and Hanus-Fajerska 2017 ). In such experiments using of seeds collected from natural habitats to initiate in vitro culture is a common practice and was previously reported for others metallophytes like Alyssum corsicum (Babaoğlu Aydaş et al 2013 ), Plantago algarbiensis and P. almogravensis (Gonçalves et al 2009 ), or Biscutella laevigata (Hanus-Fajerska et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Subcultures were done with 8-week intervals. After 16 weeks, the shoots were counted and micropropagation coefficient (MC) was calculated using the following formula, which was previously successfully used by Muszyńska and Hanus-Fajerska ( 2017 ) for another taxonomically related species belonging to Caryophyllaceae:…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, metal-rich plant biomass can be used as a “bio-ore” for recovery of precious metallic elements (phytomining, agromining), as an eco-catalysts for various chemical transformations or as a natural source of micronutrients that are essential for human diet (biofortification). Undoubtedly, there is an urgent need of protection of metalliferous sites and conservation of metallophyte biodiversity (Muszyńska et al 2017 ; Muszyńska and Hanus-Fajerska 2017 ; Wójcik et al 2017 ). During the last several years, in vitro culture techniques have been extensively developed and applied to support plant conservation (Engelman 2011 ; Kikowska et al 2014 ; Alfonso et al 2017 ; Boisson et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

Muszyńska

Hanus-Fajerska

Koźmińska

2018

Water Air Soil Pollut

Self Cite

View full text Add to dashboard Cite

In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration of such protocols is also prerequisite for selection of heavy-metal-tolerant plant material that could be afterwards used for restoration or remediation of polluted sites. In this study, culture protocol for Gypsophila fastigiata propagation was developed. The highest multiplication coefficient, which reached 6.5, and the best growth parameters were obtained on modified MS medium supplemented with 1.0 mg L−1 2iP and 0.2 mg L−1 IAA. The obtained cultures were treated with different concentrations of lead nitrate (0.1, 0.5, and 1.0 mM Pb(NO3)2) or cadmium chloride (0.5, 2.5, and 5.0 μM CdCl2). The growth parameters, photosynthetic pigments, and phenolic compound content were examined in order to evaluate whether tested metal salts can have an adverse impact on studied culture. It was ascertained that Pb ions induced growth disturbances and contributed to shoot wither. On the contrary, the proliferative shoot cultures were established on media containing Cd ions and the multiplication coefficients and shoot length increased on all media enriched with CdCl2. Chlorophylls and carotenoid contents were negatively affected by application of 5.0 μM of cadmium; nevertheless, in shoots treated with 2.5 μM CdCl2, increased accumulation of photosynthetic pigments occurred and their amount was similar to untreated culture. Adaptation to Cd was associated with stimulation of phenolic compound synthesis. Hence, we have reported on unambiguous positive result of in vitro selection procedure to obtain vigorous shoot culture tolerant to cadmium.Electronic supplementary materialThe online version of this article (10.1007/s11270-018-3702-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

Muszyńska

Hanus-Fajerska

Koźmińska

2018

Water Air Soil Pollut

Self Cite

View full text Add to dashboard Cite

show abstract

“…Efficacy of vegetative propagation and suitability of plant material for field conditions Recent years have brought many valuable studies on different aspects of agrobiotechnology (Fotlz et al 2000;Moeen and Agraval 2017), where scientific teams' leaders were ambitious enough to achieve far-reaching aims in the near future (Bell et al 2018). Desirable e ff ec t s w e r e f r e qu e n t l y pr o d u c e d , a l s o i n micropropagation of vascular plants of different taxonomic origin (Citadelli et al 2017;Muszyńska and Hanus-Fajerska 2017;Corteés-Olmos et al 2018;Suárez et al 2018). Future prospects depend on successful improvement of the techniques used for obtaining genotypes with enhanced tolerance to abiotic stresses (Wang et al 2003;Clemens 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Elaboration of protocols to ensure efficient masspropagation of populations growing in the wild is of vital importance. It also concerns numerous populations representing calamine flora or valuable metallophytes (Li et al 2010;Hanus-Fajerska et al 2012;Muszyńska and Hanus-Fajerska 2017;Muszyńska et al 2018a, b).…”

Section: Discussionmentioning

confidence: 99%

Long-term field study on stabilization of contaminated wastes by growing clonally reproduced Silene vulgaris calamine ecotype

2019

Self Cite

View full text Add to dashboard Cite

Aims Pseudo-metallophyte Silene vulgaris frequently colonizes polluted areas. We investigated whether plants obtained under in vitro conditions can be used to form long-term communities on zinc-lead tailings rich in cadmium. To this end, we checked the species stabilization capacity and biochemical properties of the area subjected to long-term cultivation of a local S vulgaris ecotype. We focused on evaluation of its ability to accumulate trace metals (TMs) after 10-year cultivation period. Methods The calamine S. vulgaris ecotype was propagated in vitro and acclimatized to ex vitro conditions in a greenhouse. The plants were then replanted on plots created on the flotation settler heavily polluted with Zn, Pb and Cd. The capacity of trace metal accumulation in plant material was checked based on translocation (TF) and bio-concentration (BCF) factors. Nutrient, TMs content, and enzyme activity of substrate were determined after 1 and 10 years of cultivation and compared with the properties of bare flotation waste. Results Numerous plants obtained through vegetative in vitro propagation were planted in the field after a short hardening period. Cultivation of plant material obtained this way increased microbial activity, C concentration, and reduced TM contents in the substrate. TF calculated after the first season of S. vulgaris cultivation was similar for all metals and amounted to about 40%. After 10 years, it was the highest for Cd (50%). Independently of the experiment duration, BCF for Zn and Pb was <1, while for Cd it reached about 1.4 after 10 years for both shoots and roots. Conclusions The calamine S. vulgaris ecotype cloned in tissue culture proved useful material for stabilization of trace metal contaminated post-flotation wastes. Ten years of its cultivation in tailings rich in Zn, Pb and Cd contributed to humus layer formation and increase in microbial activity in the substrate. Thus, the beginning of soil formation was noticed in heavily polluted tailings.

show abstract

Genetic Engineering for Metal Tolerance and Accumulation

2022

Aquatic Environmental Bioengineering

View full text Add to dashboard Cite

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

Cited by 19 publications

References 32 publications

Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

Long-term field study on stabilization of contaminated wastes by growing clonally reproduced Silene vulgaris calamine ecotype

Genetic Engineering for Metal Tolerance and Accumulation

Contact Info

Product

Resources

About