An Efficient &lt;i&gt;In Vitro&lt;/i&gt; Propagation System for Purple Cone-Flower (&lt;i&gt;Echinacea Purpurea&lt;/i&gt; L.)

Dahanayake, N.; Chen, Xiaolu; Zhao, Fei; Yang, Yuansheng

doi:10.4038/tare.v13i2.3135

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…The cytokinin-auxin combination BAP/NAA showed the greatest potential to encourage the establishment of E. purpurea shoots. A similar synergistic effect on shoot multiplication was found when BAP in combination with an auxin was applied to E. purpurea culture [7,8,24]. In our experiment, auxins (IBA or NAA) at low concentration in combination with half strength MS medium increased both the frequency of rooted shoots and the number of roots per shoot compared with the same parameters of the control medium.…”

Section: Discussionsupporting

confidence: 83%

“…IBA was the best root-inducing auxin for E. purpurea and seems to play a stimulatory role in the process of root formation in shoots, although Koroch et al [7] demonstrated rooting in plant growth regulators-free medium. Different concentrations of auxins for plant rooting of E. purpurea were effectively applied [4,23,24]. In the present study, propagated shoots were successfully acclimatized.…”

Section: Discussionmentioning

confidence: 82%

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Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

et al. 2012

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AbstractAn effective in vitro protocol for rapid clonal propagation of Echinacea purpurea (L.) Moench through tissue culture was described. The in vitro propagation procedure consisted of four stages: 1) an initial stage - obtaining seedlings on Murashige and Skoog (MS) basal medium with 0.1 mg L−1 6-benzylaminopurine, 0.1 mg L−1 α-naphthalene acetic acid and 0.2 mg L−1 gibberellic acid; 2) a propagation stage — shoot formation on MS medium supplemented with 1 mg L−1 6-benzylaminopurine alone resulted in 9.8 shoots per explant and in combination with 0.1 mg L−1 α-naphthalene acetic acid resulted in 16.2 shoots per explant; 3) rooting stage — shoot rooting on half strength MS medium with 0.1 mg L−1 indole-3-butyric acid resulted in 90% rooted microplants; 4) ex vitro acclimatization of plants. The mix of peat and perlite was the most suitable planting substrate for hardening and ensured high survival frequency of propagated plants. Significant higher levels were observed regarding water-soluble and lipid-soluble antioxidant capacities (expressed as equivalents of ascorbate and α-tocopherol) and total pnenols content in extracts of Echinaceae flowers derived from in vitro propagated plants and adapted to field conditions in comparison with traditionally cultivated plants.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 82%

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

et al. 2012

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“…Culturing of stem cuttings produces much proliferated shoots than that of leaves in Dragon fruit (Table 01). This is in agreement with Dahanayake et al (2010) and Sauve et al (2004) those reported that stem cuttings of Echinacea purpurea and stem cuttings of Echinacea tennesseensis produced much shoots from stem explants than the explants of leaves on MS basal medium supplemented with 0.3mg/l BA and 0.01mg/l NAA and supplemented with NAA at 0.54μM and thidiazuron (TDZ) respectively. Shoots were regenerated in two steps in this protocol; first explants were cultured on shoot proliferation medium and then proliferated shoots were again cultured on root induction medium.…”

Section: Discussionsupporting

confidence: 81%

“…Root induction of proliferated shoots was obtained on MS medium supplemented with and 0.01mg/l NAA regardless of the shoot induction medium. This is in agreement with Dahanayake et al (2010) and Sauve et al (2004) where they reported that root induction is independent from shoot induction.…”

Section: Discussionsupporting

confidence: 81%

Regeneration of Dragon Fruit (Hylecereus undatus) Plantlets from Leaf and Stem Explants

Dahanayake¹,

Ranawake

2012

Trop Agric Res & Ext

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Dragon fruit (Hylocereus undatus) (Cactacea) is a climbing vine which has received worldwide attention, first, as an ornamental plant and then extended as a fruit crop. Since seed viability of stored dragon fruit is very low, stem cuttings are used as planting material in Dragon fruit. Though several studies have examined different propagation methods for Dragon fruit; very little information is available on protocols for production of high quality planting material via tissue culture. In the present study we examined the potential of direct shoot regeneration of Dragon fruit explants using leaf and stem cuttings obtained from in vitro germinated seedlings in 3 different concentrations of Benzylaminopurine (BA) 2, 2.5, 3mg/l supplemented with 0.01mg/l NAA to the Murrashige and Skoog (MS) basal regeneration medium. Thereafter regenerated plantlets from direct organogenesis from all explants were rooted in MS basal medium supplemented with 0.01mg/l NAA (Naphthaleneacetic acid). Results revealed that the type of explants greatly influences the regeneration ability of shoot buds. Stem explants exhibited much higher regeneration ability (18 buds/explant) than leaf explants (3 buds/ explant) without vitrification. Within 4 weeks, buds were initiated on explants on MS basal medium supplemented with 2.5mg/l BA and 0.01mg/l NAA and buds took nearly 60 days to elongate to 1.5cm on the same medium. Stem and leaf explants, regenerated the highest number of shoots on MS medium supplemented with 2.5mg/l BA and 0.01 NAAmg/l compare to that of the other hormone combinations. Rooting was observed in regenerated mature shoots after transferred onto MS basal medium with 0.01mg/l NAA.

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“…The medium was solidified with 0.8% Agar prior to autoclaving at 1.4 kgcm -2 force for 20 minutes. The seeds were cultured under light in at 25-27 o C for 10 days (Dahanayake et al, 2010).…”

Section: Establishment Of Aseptic Culturesmentioning

confidence: 99%

Callus formation and organogenesis of tomato (Lycopersicon escu-lentum mill variety Thilina)

Manawadu

Dahanayake

Senanayake

2016

Trop Agric Res & Ext

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This study was conducted to investigate the effects of explant source and hormone concentrations on the callogenesis, calli growth, plantlet regeneration and rooting of a local tomato variety (Lycopersicon esculentum mill. variety 'Thilina'). Different combinations of Benzyl Adenine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) were used with hypocotyl, leaf and root explants in a completely randomized design with five replicates to evaluate the success in plantlet regeneration. Regenerated healthy shoots sub cultured in to MS medium with various concentrations of Indole-3-butric acid (IBA) for rooting. After one month the weight of fresh callus, number of regenerated shoots and roots were evaluated. Anova (DMRT) test shows there were significant effects at p<0.05 level. Combination of BAP (0.1mgl-1) with 2.4-D (2.0mgl-1) and hypocotyl explant produced the best quality fresh callus in highest weight. The best hormonal combination for shoot regeneration (4 shoots/explant) was 0.1mgl-1 NAA and 0.5mgl-1 Kinetin from callus. Maximum direct regeneration was observed on MS medium containing 0.5mgl-1 Kinetin, 2.0mgl-1 BAP, 0.1mgl-1 1-Naphthaleneacetic acid (NAA) and 100mgl-1 my-inositol within 15-20 days (4 shoots/explant). Leaf bud revealed to be better explants for direct regeneration. Highest root number per plantlet was observed with 2.0mgl-1 IBA.

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An Efficient <i>In Vitro</i> Propagation System for Purple Cone-Flower (<i>Echinacea Purpurea</i> L.)

Cited by 5 publications

References 11 publications

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants

Regeneration of Dragon Fruit (Hylecereus undatus) Plantlets from Leaf and Stem Explants

Callus formation and organogenesis of tomato (Lycopersicon escu-lentum mill variety Thilina)

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