An Efficient Method for Direct Shoot Regeneration from Leaf Explants of Solanum nigrum Induced by Thidiazuron

Abstract

Direct shoot regeneration was established from leaf, petiole and nodal explants from in vitro and field grown plants of Solanum nigrum on Murashige and Skoog (MS), and Gamborg's (B5) media containing different levels of plant growth regulator, Thidiazuron (TDZ). Comparison of the individual effect of basal media, levels of TDZ, explant type or source of explants on shoot regeneration was tested. Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 µM TDZ (73.70%) after 60 days of initial culture. The number of shoots was highest (35.10) with 9.13 cm longest shoot at 2.27 µM TDZ. There was a little correlation between predictors and dependent variables was found; whereas, GLM analysis demonstrated significant correlation between the factors. In vitro shoots were transferred to a half-strength MS medium containing different concentrations of NAA or IBA in MS or B5 medium. MS medium produced 100% roots supplemented with 0.5 µM NAA after 28 days. However, stout and hardy rooted shoots were obtained at 0.5 µ IBA with 10.19 mean number and 5.57 cm long roots. Rooted shoots were shifted to ex vitro conditions in polyethylene-cups filled with peat moss: sand: garden soil (1:1:1) with 85.50% survival with true-totype morphology under the natural conditions. The present investigation demonstrated that TDZ may be used for high frequency direct shoot regeneration from leaf explants of S. nigrum on B5 basal medium. This protocol may be useful for subsequent high yielding metabolites producing plants.

High frequency direct shoot regeneration from Kazakh commercial potato cultivars

“…The success of potato in vitro culture depends on the explant, culture medium, and combination and concentration of plant growth regulators (PGRs) in the culture medium ( Afrasiab, Rashid & Akram, 2017 ; AL-Hussaini, Yousif & AL-Ajeely, 2015 ; Kumlay, 2014 ; Mohapatra et al, 2018 ). Shoot culture is the basic technique of potato biotechnology.…”

Shoot Induction of Dendrobium lasianthera J.J.Smith with Several Types of Cytokinins through In Vitro Culture

“…One plausible explanation suggests that TDZ might facilitate the accumulation or synthesis of native plant growth hormones, contributing to its observed effects (Maity et al, 2016). The promotion of growth such as callus formation, shoot regeneration, and somatic embryo formation take place when applied at higher concentration and axillary proliferation at comparatively lower concentrations (Haddadi et al, 2013;Ugandhar & Venkateshwarlu, 2015;Afrasiab et al, 2017). It might be due to its similar biological activity like other N6-substituted cytokinins such as N-Ndiphenylurea and N-(2-chloro-4-pyridyl)-N′phenylurea.…”

Transformation and Expression of Na+/H+ Antiporter Vacuolar (AgNHX1) Gene in Potato (Solanum Tuberosum L.) Plants Under Salt Stress

“…However, 6-Benzylaminopurine (BAP) and thidiazoron (TDZ) are also used as plant hormones for in vitro regeneration (Ehsanpour and Jones, 2000). TDZ is a substituted Nphenyl urea which is an important regulator that promotes the maturation of somatic embryogenesis and increases the shoot induction of callus in many in vitro regeneration systems of plants (Afrasiab et al 2017). Na + /H + antiporter genes are important for improving the plant's tolerance to salinity using genetic engineering.…”