An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant

Faisal, Mohammad; Ahmad, Naseem; Anis, Mohammad

doi:10.1007/s11816-007-0025-4

Cited by 83 publications

(44 citation statements)

References 18 publications

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“…Reduction in the regeneration potential appeared to be due to detrimental effect of high concentration on the cells predetermined to form vegetative buds. Reduction in shoot number at concentration higher than the optimal level has been reported in various medicinal plants including Ruta graveolens (Faisal et al 2005b); Eclipta alba (Hussain and Anis 2006) and Tylophora indica (Faisal et al 2007). A comparison of relative effectiveness of different cytokinins for multiple shoot formation revealed the order of effectiveness as BA>Kin>2-iP.…”

Section: Discussionmentioning

confidence: 84%

Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal

Fatima

Anis

2011

Physiol Mol Biol Plants

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An efficient, rapid and improved in vitro plant regeneration protocol has been established for Withania somnifera L. using shoot tip and nodal explants, excised from 15 days old aseptic seedlings. A range of cytokinins were investigated for multiple shoot regeneration. Of the three cytokinins, 6-benzyladenine (BA), Kinetin (Kin) and 2-isopentenyl adenine (2-iP) evaluated as supplement to Murashige and Skoog (MS) medium, BA at an optimal concentration of 2.5 μM was most effective in proliferating apical and axillary buds. The highest regeneration frequency (95 %) and number of shoots (36.1±0.33) were obtained on MS medium fortified with BA (2.5 μM) and NAA (0.5 μM) from nodal segments. High frequency of rooting (100 %) was obtained in in vitro raised shoots when transferred to half-strength MS medium supplemented with NAA (0.5 μM). Histological sections revealed that additional shoot bud primordia were differentiated within the explants just underneath the suberized cells which appeared to be arrested in their development. The presence of additional bud primordia within the explants is thereby helpful to maximize the potential of this system. The regenerated plantlets with well developed shoots and roots were hardened successfully, established in earthen pots containing garden soil and maintained in greenhouse with 95 % survival rate.

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Section: Discussionmentioning

confidence: 84%

Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal

Fatima

Anis

2011

Physiol Mol Biol Plants

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“…Tylophora indica plantlets were micropropagated by the method described by Faisal et al (2007). After four weeks of rooting, uniform young plantlets with sixÁseven leaves were removed from the culture medium, washed gently under running tap water and transferred to plastic pots containing sterile soilrite.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Effect of light irradiations on photosynthetic machinery and antioxidative enzymes duringex vitroacclimatization ofTylophora indicaplantlets

Faisal

Anis

2010

Journal of Plant Interactions

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In the present communication, we studied the effect of light stress on pigment contents, net photosynthetic rate, electrolyte leakage, malondialdehyde concentration and various antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and ascorbate peroxidase (APX) during acclimatization of micropropagated Tylophora indica plantlets. Pigment (Chlorophyll a, b and carotenoids) contents in ex vitro formed leaves were found significantly higher compared to the in vitro formed ones. In vitro plantlets (day 0) exhibited a low photosynthetic activity, but with the emergence of new leaves a significant increase in net photosynthetic rates was observed. Changes in the activity of the antioxidant enzymes system were also observed during the critical days of acclimatization. Plantlets showed increased levels of SOD production, indicating its preventive mechanism of membrane oxidation and damage to biological molecules in high light (HL) acclimatized plantlets. The CAT activity increased at both low lights (LL) and HL during the whole period of acclimatization. Likewise, photoexposure of plantlets at LL and HL showed elevated activity of GR and APX against 0 day plantlets.

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“…A decrease in the number of shoots was observed by increasing the BA concentration beyond 5 mmol/L (Figure 2). Similarly, the synergism of BA in combination with NAA has been well documented in several plant species, such as Rauvolfia tetraphylla, [14] Tylophora indica, [20] Enicostemma littorale [21] and Metabriggsia ovalifolia Note: T a , annealing temperature. [22].…”

Section: Clonal In Vitro Multiplication Of a Marinamentioning

confidence: 93%

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

Alatar

Faisal

Hegazy

et al. 2015

Biotechnology & Biotechnological Equipment

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An efficient protocol for clonal multiplication of an important mangrove, Avicennia marina, was developed through in vitro culture of nodal segments obtained from a mature plant. The nodal explant induced multiple shoots when cultured on the Murashige and Skoog (MS) basal medium supplemented with varying concentrations and combinations of 6-benzyladenine (BA) and a-naphthalene acetic acid. The highest response in terms of per cent regeneration (73%), average number of shoots/explant (3.25 § 0.25) and maximum shoot length (5.2 § 0.27 cm) was obtained on the MS medium supplemented with BA 5.0 mmol/L C NAA 1.0 mmol/L C 3 g/L activated charcoal after 8 weeks of culturing. The regenerated shoots were rooted well in the MS medium supplemented with 1.0 mmol/L indole-3-butyric acid with an average of 2.9 § 0.24 roots per microshoot. The rooted plantlets were successfully transferred to pots containing normal garden soil with 70% survival rate. The genetic stability of the regenerated plants was evaluated using single primer amplification reaction (SPAR) methods viz., random amplified polymorphic DNA, directed amplification of minisatellite DNA and intersimple sequence repeat polymorphism. The SPAR analysis revealed monomorphic banding patterns in all in vitro regenerated plantlets of A. marina and similar with that of the mother tree confirming their genetic uniformity and clonal fidelity.

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An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant

Cited by 83 publications

References 18 publications

Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal

Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal

Effect of light irradiations on photosynthetic machinery and antioxidative enzymes duringex vitroacclimatization ofTylophora indicaplantlets

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

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