An efficient multiple shoot induction and genetic fidelity assessment of Exacum bicolor Roxb., an endemic and endangered medicinal plant

Ashwini, A.; Ramakrishnaiah, H.; Manohar, S. H.; Majumdar, Mala

doi:10.1007/s11627-015-9726-5

Cited by 15 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gupta et al (2020) reported maximum mean number of roots (14.1 ± 2.9) on half-strength MS medium augmented with 0.2 mg/L IBA in Moringa oleifera, whereas, Kher and Nataraj (2019) observed maximum average number of roots (19.8 ± 0.9) on half strength MS with 2 mg/L IBA within 28 d of culture in Pluchea lanceolata. There are several other investigations, where, IBA was found to be effective for the rhizogenesis in various plant systems such as Cucumis sativus (Sangeetha and Venkatachalam 2014), Exacum bicolor(Ashwini et al 2015), Plumbago zeylanica Effect of different concentrations of auxins on in vitro roots formation in root callus-derived excised shoots of R. hastatus cultured on MS medium for 30 d.…”

mentioning

confidence: 99%

Optimization of micropropagation protocol and elicitation of luteolin and rutin biomolecules using phenylalanine and chitosan in root callus of Rumex hastatus D. Don

Singh

Agrawal

2022

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Rumex hastatus D. Don, a traditional medicinal plant known for its, antitumorous, antiangiogenic and antibacterial potential is continuously under threat of extinction due to over exploitation and climatic changes. Phytochemical analysis of different plant parts showed that roots had the maximum quantity of two bioactive molecules, luteolin and rutin and thus was selected for establishment of aseptic culture.In vitro technology has been employed for developing an e cient micropropagation protocols for its conservation, large scale production as well elicitation of the valuable bioactive compounds. Murashige and Skoog's medium supplemented with 5µM TDZ proved best for developing greenish-brown and compact calluses in 98% root cultures within 30 days. Such callus pieces on subculture to same but fresh medium differentiated an average of 10.50 ± 1.29 shoots which were elongated on MS basal medium. Such excised shoots, rooted in MS + 0.1 µM IBA medium with an average of 12.03 ± 2.49 roots per shoot.These plantlets were successfully acclimatized under greenhouse conditions and elds and their genetic delity was evaluated employing SRAP and SCoT markers which revealed their high similarity with mother plant proving their true-to-type nature. Interestingly, the in vitro regenerants showed elevated levels of phenols, avonoids, luteolin and rutin content and higher antioxidant activities over the mother plant as done by phosphomolybdenum and DPPH assays. For elicitation of bioactive compounds, 100 mg/L phenylalanine was best for enhancing optimum luteolin content (407.18 ± 10.58 µg/g d.w.) over control (22.10 ± 0.57 µg/g d.w.) almost, 17.42-fold, whereas for rutin, 25 mg/L chitosan proved most effective enhancing its synthesis up to 1169.07 ± 46.49 µg/g d.w. over control (312.41 ± 12.42 µg/g d.w.), i.e., 2.74-fold. To the best of our knowledge, this is our rst report on micropropagation and elicitation of luteolin and rutin in Rumex hastatus through root culture.

show abstract

mentioning

confidence: 99%