An Efficient Protocol for Callus Induction in &lt;i&gt;Aquilaria malaccensis&lt;/i&gt; Lam. Using Leaf Explants at Varied Concentrations of Sucrose

Saikia, Moitreyee; Shrivastava, Karuna; Singh, S. S.

doi:10.5923/j.plant.20120206.03

Cited by 12 publications

(7 citation statements)

References 4 publications

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“…Higher fresh weight indicates the higher amount of biomass cell in the callus. There were so many similar results have been reported [29], [44], [45]. Callus cultures were extremely important in plant biotechnology.…”

Section: B Establishment Of Callus Inductionsupporting

confidence: 65%

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Salam*¹,

Awal²,

Abdullah³

2019

IJEAT

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Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

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Section: B Establishment Of Callus Inductionsupporting

confidence: 65%

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Salam*¹,

Awal²,

Abdullah³

2019

IJEAT

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“…Similar wounding is also common in upper Assam, especially among traditional growers (Saikia et al 2012). Nevertheless, such efforts do not yield highly productive results and the agarwood produced is of inferior quality (Persoon 2007).…”

Section: Discussionmentioning

confidence: 98%

“…Artificial wounding is believed to stimulate oleoresin formation in agarwood (Saikia et al 2012). Infection by fungi is also considered to be associated with resin formation in agar trees (Oldfield et al 1998).…”

Section: Discussionmentioning

confidence: 98%

Management and Economic Aspects of Growing Aquilaria agallocha Roxb. in Bangladesh

Rahman

Nath

Sarker

et al. 2015

Small-scale Forestry

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The agar tree (Aquilaria agallocha Roxb.) is an evergreen tropical tree species containing precious dark resinous heartwood (agarwood). The population of agar tree is rapidly declining in natural forests in Bangladesh, and the species is now being planted in government-owned degraded forest sites as well as privatelyowned homestead forests. A survey was conducted of 120 agar farmers and 20 agarbased enterprises in the Moulvibazar district of Bangladesh, to investigate the management system of agar plantations and agar-based oil enterprises and the financial viability of both sectors. It was found that the traditional management practices were adapted based mostly on indigenous knowledge and technology to manage agar plantations and agar-based oil enterprises. Discounted cash flow analysis indicated that both agar plantations and agar-based oil enterprises are financially viable. The net present value of 1 ha of agar plantation (rotation period 12 years) was estimated to be Tk. 4.9 million and the net annual return from agarbased enterprises was estimated to be Tk. 0.8 million (Tk. 78 = 1 US dollar). Despite some threats to agar farming posed by the traditional wounding techniques of agar trees and the involvement of intermediaries in the marketing channel of agar trees and agar-based products, this economic sector has potential to play a vital role in rural peoples' livelihood subsistence and forestry sector development in Bangladesh.

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“…IAA dan IBA merupakan auksin alami yang disintesis oleh tumbuhan, efektif untuk inisiasi akar adventif, tetapi tidak terlalu efektif untuk pembentukan kalus, sedangkan 2,4-D yang merupakan auksin sintetik yang berfungsi sebaliknya, memiliki pengaruh kuat dalam membentuk kalus (George, 1993). Penggunaan kombinasi auksin dan sitokinin sangat penting dalam pembentukan kalus gaharu, dimana adanya sitokinin dengan konsentrasi yang lebih tinggi dapat memicu pembelahan sel (Ito et al, 2005;Qi et al, 2005;Saikia et al, 2012;Daud, 2013;Khairunisa, 2013;Zalikha, 2013;Jayaraman et al, 2014). Kombinasi yang terbaik untuk menginduksi kalus A. crassna diperoleh pada medium MS dengan penambahan 10 -6 M 2,4-D dan 10 -6 M BA (Okudera and Ito, 2009), sedangkan kombinasi 2 mg/L 2,4-D dan 0,1 mg/L kinetin dapat menginduksi kalus dari daun A. malaccensis hingga 70-73% dengan biomassa yang tinggi dan tumbuh cepat (Saikia et al, 2013).…”

Section: Pembahasanunclassified

INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

Leksonowati¹,

Witjaksono²,

Ratnadewi³

2017

BERITABIOLOGI

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Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage. The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

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An Efficient Protocol for Callus Induction in <i>Aquilaria malaccensis</i> Lam. Using Leaf Explants at Varied Concentrations of Sucrose

Cited by 12 publications

References 4 publications

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Management and Economic Aspects of Growing Aquilaria agallocha Roxb. in Bangladesh

INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

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