An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments

Abstract

The influence of the basal medium and different plant growth regulators on micropropagation of nodal explants from mature trees of lemon cultivars was investigated. Although the basal medium did not affect any of the variables, explants on DKW medium were greener. Several combinations of 6-benzyladenine (BA) and gibberellic acid (GA) were used to optimise the proliferation phase. The number of shoots was dependent on the BA and GA concentrations and the best results were obtained with 2 mg l -1 BA and 1 or 2 mg l -1 GA. Explants length was shorter with the higher BA concentrations and, in all genotypes, shoot length was greater with 2 mg l -1 GA. The best results for productivity (number of shoots 9 the average shoot length) were obtained with 2 mg l -1 BA and 2 mg l -1 GA, although explants with chlorosis and narrow leaves were observed. The presence of BA and GA in the proliferation medium was essential for the explant multiplication but GA had a greater influence. The transfer of in vitro shoots to rooting media, containing different concentrations of indole butyric acid (IBA) and indole acetic acid (IAA) produced complete plantlets. Lemon shoots rooted well in all rooting combinations. The highest rooting percentages were obtained on media containing 3 mg l -1 IBA alone or IBA in combination with 1 mg l -1 IAA and on these media the highest numbers of roots were produced. The average root length was affected significantly by the IBA and IAA concentrations. Root length was greater when only 3 mg l -1 IBA was used, and in this rooting medium explants had a better appearance, with greener and larger leaves. The success during the acclimatisation was close to 100% and the plantlets exhibited normal growth in soil under greenhouse conditions.

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

“…These results indicated that auxin-combined treatments could stimulate root induction frequency more than the individual auxin treatments, thus indicating a synergistic effect for inducing leaf explants-derived adventitious root cultures of G. procumbens. The result was in agreement with that described for different species in reports by Khosh-Khui and Sink (1982) and Pérez-Tornero et al (2010).…”

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

“…Tissue culture and micropropagation protocols have been described for a number of Citrus spp. using a wide range of explant sources (Grinblat 1972;Barlass and Skene 1982;Duran-Vila et al 1989;Beloualy 1991;Carimi et al 1995;Altaf and Ahmad 1997;Normah et al 1997;Al-Khayri and Al-Bahrany 2001;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Altaf et al 2009a, b;Khan et al 2009;Laskar et al 2009;Sharma et al 2009;Pe'rez-Tornero et al 2010;Singh and Rajam 2009;. However, a little work has been carried out on the tissue culture of C. jambhiri (Raman et al 1992;Altaf and Ahmad 1997;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Sharma et al 2009;Savita et al 2010).…”

Application of silicon in plant tissue culture

“…It has been reported that Si inclusion can increase the levels of both GA 1 and GA 20 in rice cultivars (Hwang et al 2007). It has been indicated that shoot induction in citrus (Pérez-Tornero et al 2010), apple (Isogai et al 2008), and Cephaelis ipecacuanha (Kaushal et al 2005) is affected by GA 3 . On the other hand, Si positively affects shoot induction; therefore, this effective role of Si may result from altering endogenous levels of Gas.…”