An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol A in milk samples

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

“…Compared to bare nanoparticles used in MA detection illustrated above such as AuNPS [63] and AgNPs [70], this method promises the reliability, accuracy and detection time of MA in milk samples because aptamer has ability to stabilize NPs in salts. In addition, the LOD of this method possesses same range of that LOD reported for biosphenol detection in water samples [77]. However, its LOD is lower probably due to the complexity of milk composition which may affect the sensitivity of target induced-AuNPs aggregation ( Figure 3).…”

“…In food safety monitoring, aptamers are applied for detection of chemical and/or biological hazards. Illegal additives used in food adulteration such as 17-β estradiol and bisphenol A were detected using DNA aptamers5'-GGGCCGTTC-GAACACGAGCATGCCGGTGGGTGGTCAGGTGGGATA GCGT-3' and 5'-TCCGCGAATTACACGCAGAGGTAGCGGCTCTGCG-CATTCAATTGCTG-3' combined with 5'-CGCGCTGAAGCGCG-GAAGC-3', respectively [77,86,87]. This method is based on competitive recognition of bisphenol with immobilized aptamer fixedon the surface of the electrode.…”

Aptamer-Based Technology for Food Analysis

“…Yildirim et al [125] prepared a portable, evanescent, wave fiber-optic aptasensor for rapid, on-site detection of BPA, with a low detection limit of 1.86 nM. In particular, based on gold nanoparticles dotted graphene modified glassy carbon electrode, Zhou et al [126] fabricated an electrochemical aptasensor for label-free detection of bisphenol A in milk samples, with the detection limit of 5 nM.…”

Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum

“…These chromatography and mass spectrometry-based techniques can analyze BPA with high sensitivity, however, large capital investment, skilled operators, and cumbersome sample preparation and pretreatment are required, reducing their applicability of on-site and rapid detection of BPA in various samples and matrices [9,11]. The other recently developed methods for BPA detection include enzyme-linked immunosorbent assay (ELISA) [12], fluorescent method [13], surface-enhanced Raman scattering [14,15], colorimetric methods [16], and electrochemical sensors [17][18][19][20][21][22][23][24][25][26][27][28][29]. Among those methods, electrochemical sensors are the widely accepted and employed group due to their simple operation, rapid response time, low cost, high sensitivity, potential for miniaturization, and capability of real sample analysis [9], thereby having promising potential for BPA analysis in human internal fluids.…”

“…To enhance the detectable signals, nanomaterials that possess large specific surface area and electrocatalytic property are usually utilized to catalyze the redox reaction of BPA. Electrochemical sensors that were developed with metal nanoparticles [18], quantum dots [19], carbon nanotubes [20,21], graphene [17,[22][23][24], and the composite of nanomaterials [25][26][27] have been reported for the detection of BPA. Besides BPA, however, there are many other phenolic compounds that also can be electrocatalyzed by the nanomaterials mentioned above, which may interfere the detection of BPA.…”