An electrochemical biosensor based on cobalt nanoparticles synthesized in iron storage protein molecules to determine ascorbic acid

Abstract

The electrochemical detection of ascorbic acid (AA) was investigated using a cobalt(III)-ferritin immobilized on a self-assembled monolayer modified gold electrode in phosphate buffer solution (pH 7.5). The modified electrode showed excellent electrochemical activity for oxidation of AA. The response to AA on the modified electrode was examined using cyclic and differential pulse voltammetry techniques. The resulting biosensor showed a linear response to AA in a concentration range from 6.25×10 to 2.31×10 M with sensitivity of 86,437 μAM and detection limit of 4.65 × 10 M based on a signal-to-noise ratio of 3. Electrochemical parameters including the charge transfer coefficient (α) and the apparent heterogeneous electron transfer rate constant (k ) for AA were found to be 0.52 and 1.054 Sec , respectively. It has been shown that, using this modified electrode, AA can be determined with high sensitivity, low detection limit, and high selectivity.

A novel sensitive laccase biosensor using gold nanoparticles and poly L‐arginine to detect catechol in natural water

“…Moreover, the potential scan rate and redox peak currents were in a linear relationship from 40 to 100 mV Sec −1 ; demonstrating that the redox reactions were controlled by adsorption (Fig. a) .…”

Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support

“…By increasing the scan rate, the amounts of redox potentials have changed; the cathodic and anodic potentials shifted to negative and positive amounts, respectively (figure 5b). Transfer coefficient (α) and the standard heterogeneous rate constant (k s ) affect the observed behaviour when the rate of the charge-transfer reaction has changed slowly [47,48]. Figure 5b shows the E a p and E c p relationship with the logarithm of the scan rate (log υ) for the proposed biosensor in the presence of catechol (1 mM).…”