An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

Abstract

A nitric oxide (NO) biosensor based on silver nanoparticles was fabricated with high sensitivity and selectivity as well as stability. Silver nanoparticles could preserve the microstructures of hemoglobin, but the electrochemical reactivity of the protein and its detection sensitivity toward NO could be greatly enhanced. Accordingly, a NO biosensor was developed. The linear concentration range was from 1.0 × 10 -6 to 5.0 × 10 -5 M. Its detection limit was 3.0 × 10 -7 M with a sensitivity of 0.0424 µA µM -1 NO. The possible co-existing compounds would not interfere with the detection.

Glucose oxidase/colloidal gold nanoparticles immobilized in Nafion film on glassy carbon electrode: Direct electron transfer and electrocatalysis

“…Recently, there is an increasing interest in using all kinds of nanoparticles to construct electrochemical biosensors [18][19][20][21] since they have high specific surface area, good biocompatibility, and good conductivity. It was reported that gold nanoparticles (GNPs) could adsorb redox enzymes (proteins) without loss of their biological activities [22].…”

Electron transfer and interfacial behavior of redox proteins

“…Therefore, silver NPs have been used in this lab as a film material to immobilize Hb [76] and Mb [77], where rapid, quasi-reversible, one-electron, heterogeneous ET processes of the proteins could be achieved. With the help of silver NPs, the ET rate of Cyt c could be very high at the redox potential of 24 mV (vs. SCE) [78], close to that of Cyt c inside mitochondrial membranes.…”

Metal Nanoparticles: Applications in Electroanalysis

“…These sensors have allowed for the indirect detection of hydrogen peroxide, using either horseradish peroxidase [104,110] or myoglobin [111], and in the sensing of nitrite or nitric oxide using hemoglobinmodified electrodes [112,113].…”