An electrochemical deoxyribonucleic acid biosensor for rapid genotoxicity screening of chemicals

Wu, Lidong; Lu, Xianbo; Wang, Xue; Song, Yi; Chen, Jiping

doi:10.1039/c5ay00020c

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…[1][2][3][4][5][6] Wu et al suggested a sensitive electrochemical biosensor for detection of double-stranded deoxyribonucleic acid. 7 Li et al reported a uorescence biosensor with an ultra-low limit of detection for folate receptors, which can be used for the observation of early cancer. 8 In addition, Shtenberg et al studied nanostructured porous Si sensors for the detection of heavy metal ions in water by immobilising horseradish peroxidase, laccase and multi-copper oxidase.…”

Section: Introductionmentioning

confidence: 99%

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

et al. 2019

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“…MB is often used for the description of the DNA damages caused by chemical factors. Thus, changes in the DNA intercalation and substitution of MB molecules with genotoxic species was recorded using voltammetric techniques for the assessment of polychlorinated dibenzodioxins, dibenzofurans and related compounds .…”

Section: Resultsmentioning

confidence: 99%

Voltammetric Detection of Oxidative DNA Damage Based on Interactions between Polymeric Dyes and DNA

et al. 2016

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Oxidative damage of DNA was assessed with glassy carbon electrode (GCE) coated with electropolymerized Methylene Blue (MB). For this purpose, DNA solution was first mixed with an oxidant (Fenton reagent, H2O2 alone and in the presence of Cu(II) ions) and then placed on the polymeric film surface. After washing, the cyclic voltammogram was recorded in buffer solution and the anodic peak potential measured against that before the contact with DNA. Oxidative DNA damage resulted in remarkable decrease of the anodic peak potential which depended on the oxidant nature and incubation period. Specificity of the DNA – MB interactions was confirmed by surface plasmon resonance (SPR) measurements. Similar experiment performed with polymerized Methylene Green (MG) and Neutral Red (NR) showed lower selectivity of the response toward various sources of reactive oxygen species. The protocol of the DNA damage detection was tested on the estimation of antioxidant properties of green tea extract and red table wine.

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“…Following rinsing, the electrodes were cleaned ultrasonically three times with ethanol followed by Milli-Q water. They were then electrochemically activated in 0.5 mol L −1 H 2 SO 4 by potential scanning between 0 and 1.7 V until a repeatable CV was achieved, after which they were thoroughly washed with Milli-Q water and dried under a nitrogen stream [ 32 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

Sun

Niu

et al. 2022

Biosensors

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Rapid and sensitive detection of cancer biomarkers is crucial for cancer screening, early detection, and improving patient survival rate. The present study proposes an electrochemical gene-sensor capable of detecting tumor related TP53 gene mutation hotspots by self-assembly of sulfhydryl ended hairpin DNA probes tagged with methylene blue (MB) onto a gold electrode. By performing a hybridization reaction with the target DNA sequence, the gene-sensor can rearrange the probe’s structure, resulting in significant electrochemical signal differences by differential pulse voltammetry. When the DNA biosensor is hybridized with 1 μM target DNA, the peak current response signal can decrease more than 60%, displaying high sensitivity and specificity for the TP53 gene. The biosensor achieved rapid and sensitive detection of the TP53 gene with a detection limit of 10 nmol L−1, and showed good specific recognition ability for single nucleotide polymorphism (SNP) and base sequence mismatches in the TP53 gene affecting residue 248 of the P53 protein. Moreover, the biosensor demonstrated good reproducibility, repeatability, operational stability, and anti-interference ability for target DNA molecule in the complex system of 50% fetal bovine serum. The proposed biosensor provides a powerful tool for the sensitive and specific detection of TP53 gene mutation hotspot sequences and could be used in clinical samples for early diagnosis and detection of cancer.

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An electrochemical deoxyribonucleic acid biosensor for rapid genotoxicity screening of chemicals

Abstract: A schematic diagram of the E-DNA biosensor detection of PCDD by DPV.

Cited by 6 publications

References 42 publications

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

Voltammetric Detection of Oxidative DNA Damage Based on Interactions between Polymeric Dyes and DNA

Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

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