An electrochemical immunosensor for cardiac Troponin I using electrospun carboxylated multi-walled carbon nanotube-whiskered nanofibres

Rezaei, Babak; Shoushtari, Ahmad Mousavi; Rabiee, Mohammad; Uzun, Lokman; Mak, Wing Cheung; Turner, Anthony

doi:10.1016/j.talanta.2018.01.046

Cited by 93 publications

(42 citation statements)

References 35 publications

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“…Moreover, it can be seen different hybrid materials which contain CNTs in immuno-sensing applications, as in the before-mentioned glucose and urea sensing applications [109][110][111]. For instance, Rezaei et al [112] have developed a highly sensitive immunosensor for use in the diagnosis of cardiovascular diseases. They stated that CNTs offer a high surface area for immobilization by embedding into nanofibers (WNF), which they used as transducer platforms.…”

Section: Immunosensors Based On Functionalized Cntsmentioning

confidence: 99%

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Nano-carbons in biosensor applications: an overview of carbon nanotubes (CNTs) and fullerenes (C60)

2020

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Recently, the use of carbon nanotubes (CNTs) and fullerenes in the design of new biosensors have attracted great interest in the development of carbon nanomaterials. Due to the superior properties of CNTs and fullerenes, the use of sensor components allows the development of reliable, accurate and fast biosensors. Depending on the types of target molecules, the development and application areas of the sensors vary. This review summarizes the role of CNTs and fullerenes in the development of biosensors in different application areas. Considering the difference between other members of the nano-carbon family, we explain why CNTs are used more widely in biosensor applications and why fullerenes have high potentials in these areas of application. Moreover, we focused on investigating the function of these nano-carbons in the detection of various analytes in bio-sensing. By discussing the challenges and future expectations, we have put forward a perspective that may help synthesize advanced composites in the development of new generation designs in biosensor applications.

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Section: Immunosensors Based On Functionalized Cntsmentioning

confidence: 99%

“…10. As a result, they developed an immunosensor that exhibits good selectivity, reproducibility, wide detection range properties [112]. Fig.…”

Section: Immunosensors Based On Functionalized Cntsmentioning

confidence: 99%

Nano-carbons in biosensor applications: an overview of carbon nanotubes (CNTs) and fullerenes (C60)

2020

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“…Thus, the incubation times of NDV and PAb/NDV-Cu(I)/Cu(II)-Chi-Gra were optimized separately. To optimize the NDV incubation time, different incubation times (5,10,15,20,30,40,50, and 60 min) were used, and after incubation with NDV, the immunosensors were incubated with PAb/NDV-Cu(I)/Cu(II)-Chi-Gra for 60 min. Finally, the immunosensors were used for DPV detection.…”

Section: Electrochemical Characterization Of the Immunosensor Cyclicmentioning

confidence: 99%

Electrochemical immunosensor with Cu(I)/Cu(II)-chitosan-graphene nanocomposite-based signal amplification for the detection of newcastle disease virus

Huang

Xie

Huang³

et al. 2020

Sci Rep

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An electrochemical immunoassay for the ultrasensitive detection of Newcastle disease virus (NDV) was developed using graphene and chitosan-conjugated Cu(I)/Cu(II) (Cu(I)/Cu(II)-Chi-Gra) for signal amplification. Graphene (Gra) was used for both the conjugation of an anti-Newcastle disease virus monoclonal antibody (MAb/NDV) and the immobilization of anti-Newcastle disease virus polyclonal antibodies (PAb/NDV). Cu(I)/Cu(II) was selected as an electroactive probe, immobilized on a chitosangraphene (Chi-Gra) hybrid material, and detected by differential pulse voltammetry (DPV) after a sandwich-type immune response. Because Gra had a large surface area, many antibodies were loaded onto the electrochemical immunosensor to effectively increase the electrical signal. Additionally, the introduction of Gra significantly increased the loading amount of electroactive probes (Cu(I)/ Cu(II)), and the electrical signal was further amplified. Cu(I)/Cu(II) and Cu(I)/Cu(II)-Chi-Gra were compared in detail to characterize the signal amplification ability of this platform. The results showed that this immunosensor exhibited excellent analytical performance in the detection of NDV in the concentration range of 10 0.13 to 10 5.13 eiD 50 /0.1 mL, and it had a detection limit of 10 0.68 eiD 50 /0.1 mL, which was calculated based on a signal-to-noise (S/N) ratio of 3. The resulting immunosensor also exhibited high sensitivity, good reproducibility and acceptable stability. Newcastle disease virus (NDV) is a viral disease of poultry that belongs to avian paramyxovirus 1. It is a singlestrand, non-segmented, and negative-sense RNA virus 1 , and it is a great threat to the poultry industry 2. The first important step in NDV prevention and control is to develop a rapid and sensitive method for diagnosis. Currently, several methods for detecting NDV, included virus isolation 3 , reverse transcription polymerase chain reaction (RT-PCR) 4 , real-time RT-PCR 5 , immunochromatographic strip (ICS) tests 6 , and reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays 7 , have been reported. However, these diagnostic methods had some disadvantages; for example, virus isolation is the gold standard for the detection of NDV, but the procedure is time-consuming. For RT-PCR, appropriate laboratory facilities and a trained technician are needed. Real-time RT-PCR requires complicated operations as well as expensive reagents and equipment. Therefore, these diagnostic methods are limited in practical applications. Electrochemical immunosensors are powerful tools that have good specificity, high sensitivity, good precision, and simple instrumentation; give rapid and reliable responses; and are relatively low cost. Their use in clinical diagnosis, food analysis, environmental monitoring and archaeological studies should be highly valuable 8. Furthermore, electrochemical immunosensors are based on antibody-antigen reactions. Therefore, immobilizing antibodies or antigens on a transducer as a biorecognition element plays a very important ...

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“…Immunosensors are condensed analytical biosensor devices which are based on ligand affinity, where immunochemical reaction interaction and molecular recognition between antigen and antibodies is coupled together to a transducer for selective detection of several kinds of proteins [114]. The advancement of immunosensors technology is an achievement in the biomedicine and till today it continues to be an exciting area of research.…”

Section: Cnts-immunosensorsmentioning

confidence: 99%

A review on carbon nanotubes in biosensor devices and their applications in medicine

et al. 2018

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In recent years, integrating biological components in analytical instruments especially in biomedical research has become a prerequisite for early diagnosis of many diseases. It is well known that the material properties (electrical and physical) of CNTs is very sensitive to be affected by exposure to biomolecules and this led to the investigation by many researchers. Though the CNT-based biosensors has been widely used due their better performance, it still has many practical concerns in application. For the successful commercialization of the concept of CNT-based biosensors, many hurdles need to overcome. Modifications on CNT biosensors have experienced a dramatic change with outstanding developments. The present article provides an overview on the recent development in CNT biosensors and comprehensive analysis was given on various ways to improve the performance of CNT with new designs. In addition, some of the practical applications and concerns in the field are addressed. The scientific and technological challenges in the field are discussed in the conclusion.

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An electrochemical immunosensor for cardiac Troponin I using electrospun carboxylated multi-walled carbon nanotube-whiskered nanofibres

Cited by 93 publications

References 35 publications

Nano-carbons in biosensor applications: an overview of carbon nanotubes (CNTs) and fullerenes (C60)

Nano-carbons in biosensor applications: an overview of carbon nanotubes (CNTs) and fullerenes (C60)

Electrochemical immunosensor with Cu(I)/Cu(II)-chitosan-graphene nanocomposite-based signal amplification for the detection of newcastle disease virus

A review on carbon nanotubes in biosensor devices and their applications in medicine

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