2020
DOI: 10.3390/mi11070660
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An Electrochemical Immunosensor for Sensitive Detection of the Tumor Marker Carcinoembryonic Antigen (CEA) Based on Three-Dimensional Porous Nanoplatinum/Graphene

Abstract: Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. The quantitative detection of a low concentration of CEA has important medical significance. In this study, three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites were prepared by a wet chemical method and modified on an electrode with enhanced conductivity, a large surface area, and good adsorption of immobilizing antibodies (Ab1). Horseradish peroxidase (HRP)-functionalized Au nan… Show more

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Cited by 32 publications
(20 citation statements)
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“…Memristive devices used in biosensing are usually functionalized with antibodies to detect antigens through a change in the voltage gap in the devices' response due to antibody-antigen interaction. Four main techniques exist in the literature for the attachment of antibodies on the surface of a device, namely direct adsorption [20], [45], [46], affinity approach [47], covalent attachment using Glycidoxypropyltrimethoxysilane (GPTS) [18,48,49], covalent attachment using 1-(3-(Dimethylamino)propyl)-3ethyl carbodiimide hydrochloride (EDC) and Nhydroxysulfosuccinimide (NHS) [16,[50][51][52][53][54][55], and noncovalent attachment [17]. The direct adsorption technique usually starts by treating the device with oxygen plasma for 15 minutes to increase hydroxyl groups (OH-).…”
Section: B Bio-functionalization Of Antibodiesmentioning
confidence: 99%
“…Memristive devices used in biosensing are usually functionalized with antibodies to detect antigens through a change in the voltage gap in the devices' response due to antibody-antigen interaction. Four main techniques exist in the literature for the attachment of antibodies on the surface of a device, namely direct adsorption [20], [45], [46], affinity approach [47], covalent attachment using Glycidoxypropyltrimethoxysilane (GPTS) [18,48,49], covalent attachment using 1-(3-(Dimethylamino)propyl)-3ethyl carbodiimide hydrochloride (EDC) and Nhydroxysulfosuccinimide (NHS) [16,[50][51][52][53][54][55], and noncovalent attachment [17]. The direct adsorption technique usually starts by treating the device with oxygen plasma for 15 minutes to increase hydroxyl groups (OH-).…”
Section: B Bio-functionalization Of Antibodiesmentioning
confidence: 99%
“…In such circumstances, although these infectious agents are present, they do not generate enough available Abs in the blood, so the concentration of the Abs in In this regard, Table 1 contains a list of recent researches in the literature of biosensors for medical applications using antibodies and aptamers as bioreceptors. It is worthy notable that these biomolecules facilitate the biosensing of analytes at concentrations as low as some femtograms per milliliter [86][87][88][89][90]. Note: *NPs = Nanoparticles…”
Section: Efficiency Of Immunosensors and Aptasensorsmentioning
confidence: 99%
“…If this indicator is significantly elevated, it can indicate a gastrointestinal tumor. The increase in CA19-9 is especially significant in gastric cancer with liver metastasis and pancreatic metastasis, which is better than other tumor markers [ 5 , 6 , 7 , 8 ]. Clinical methods such as chemiluminescence (CL) and electrochromic luminescence (ECL) are mainly used to detect CA19-9 [ 9 , 10 , 11 , 12 ], but there are shortcomings such as expensive reagents and cumbersome operation.…”
Section: Introductionmentioning
confidence: 99%