An electroenzymatic l-glutamate microbiosensor selective against dopamine

Hamdi, Naser; Wang, Jianjun; Walker, Edward T.; Maidment, Nigel T.; Monbouquette, Harold G.

doi:10.1016/j.jelechem.2006.03.022

Cited by 68 publications

(46 citation statements)

References 53 publications

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“…[5,6] Recently, amperometric biosensors based on the detection of enzymatically generated H 2 O 2 have been developed for a wide range of analytes. [7,8] Oxidase reacts with the analyte generating H 2 O 2 , which subsequently is electrooxidized at the electrode leading to a current signal. The main characteristics of the OPPy films are that the PPy polymer is dedoped, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination

Debiemme‐Chouvy

Gallois

2010

Surface & Interface Analysis

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In the design of amperometric biosensor based on the electrochemical detection of enzymatically generated H 2 O 2 , an inert membrane is often present on the electrode in order to separate it from the solution to prevent interferences. The inert membrane could be an overoxidized polypyrrole film. Generally, the overoxidized polypyrrole films are obtained in two steps, the first one being the electrosynthesis of the polypyrrole film and the second one its overoxidation in an indifferent electrolyte. In this article, a new electrochemical one-step process allowing the formation of a very thin overoxidized polypyrrole film is used. This film is characterized by SEM, XPS and cyclic voltammetry in the presence of monoelectronic reversible redox couples. Actually, the film is an electrical insulating compact layer about 10-nm thick and pin hole free. This overoxidized polypyrrole membrane was tested on one hand for the rejection of anions and large molecules such as Fe(CN) 6 3/4− and Ru(NH 3 ) 6 2/3+ , respectively, and on the other hand, for the determination of hydrogen peroxide. The results show a very good efficiency of the membrane for the rejection of the redox species tested. Finally, it is shown that the membrane/Pt electrode allows quantitative electrooxidation of H 2 O 2 . Indeed, using this electrode, under potentiostatic condition, a linear response of the anodic current in function of the H 2 O 2 electrolyte concentration was found.

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Section: Introductionmentioning

confidence: 99%

“…Sometimes the latter step could last for several hours. [8] Moreover, the thickness of the membrane is high, about 0.1-1 µm.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination

Debiemme‐Chouvy

Gallois

2010

Surface & Interface Analysis

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“…Among various detection methods, the amperometric enzyme-based biosensor probably is the best choice for biochemical analysis due to its good selectivity, high sensitivity, rapid response, convenient measurement, miniature size, and reproducible results (Hamdi et al, 2006). In order to reuse the expensive recognized biomolecules, the enzyme has to be immobilized on the electrode.…”

Section: The Immobilization Of Enzymes and Mediatorsmentioning

confidence: 99%

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Nien¹,

Chen²,

Ho³

2010

Intelligent and Biosensors

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“…[6][7][8][9] Among these, enzymatic glucose biosensor is one of the best decision due to rapid response, high sensitivity and selectivity. 10) Sensitivity is depended on the active surface area per unit area. Nanoporous materials were used for biosensors and other applications such as fuel cell, 11) chemical sensor, 12) catalysis 13) for enhancing the surface area.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Glucose Sensor Realized by Conducting Polymer Modified Nanoporous PtZn Alloy Electrode

Jo¹,

Piao²,

Son³

2013

Journal of Electrochemical Science and Technology

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:Platinum is a well known element which shows a significant electrocatalytic activity in many important applications. In glucose sensor, because of the poisoning effect of reaction intermediates and the low surface area, the electrocatalytic activity towards the glucose oxidation is low which cause the low sensitivity. So, we fabricate a nanoporous PtZn alloy electrode by deposition-dissolution method. It provides a high active surface and a large enzyme encapsulating space per unit area when it used for an enzymatic glucose sensor. Glucose oxidase was immobilized on the electrode surface by capping with PEDOT composite and PPDA. The composite and PPDA also can exclude the interference ion such as ascorbic acid and uric acid to improve the selectivity. The surface area was determined by cyclic voltametry method and the surface structure and the element were analyzed by Scanning Electron Microscope (SEM) and Energy Dispersive X-ray spectroscopy (EDX), respectively. The sensitivity is 13.5 µA/mM cm 2 . It is a remarkable value with such simply prepared senor has high selectivity.

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An electroenzymatic l-glutamate microbiosensor selective against dopamine

Cited by 68 publications

References 53 publications

Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination

Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Highly Sensitive and Selective Glucose Sensor Realized by Conducting Polymer Modified Nanoporous PtZn Alloy Electrode

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An electroenzymatic l-glutamate microbiosensor selective against dopamine

Cited by 68 publications

References 53 publications

Characterization of a very thin overoxidized polypyrrole membrane: application to H2O2 determination

Characterization of a very thin overoxidized polypyrrole membrane: application to H2O2 determination

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Highly Sensitive and Selective Glucose Sensor Realized by Conducting Polymer Modified Nanoporous PtZn Alloy Electrode

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Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination

Characterization of a very thin overoxidized polypyrrole membrane: application to H₂O₂ determination