An Electron Microscopic Correlation of Structure with Function in the Isolated Perfused Cow Adrenal, Preliminary Observations

Luft, John H.; Hechter, Oscar

doi:10.1083/jcb.3.4.615

Cited by 68 publications

(8 citation statements)

References 10 publications

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“…There are several reports of experimentally produced alterations in mitochondrial structure. Swelling occurs as a non-specific change in normal cells after the blood supply is curtailed, and appears to be reversible if perfusion with oxygenated blood ensues (8). Thyroid hormone exerts a similar effect which is also reversible (4), and glutathione in homogenates also causes mitochondrial swelling (5).…”

Section: Discussionmentioning

confidence: 99%

Corpus Intra Cristam: A Dense Body Within Mitochondria of Cells in Hyperplastic Mouse Epidermis

Frei

Sheldon

1961

The Journal of Cell Biology

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This communication reports the occurrence of dense bodies within the eristaO of many mitochondria in the stratum germinativum and stratum granulosum of hyperplastie mouse epidermis. We have seen a variety of forms which suggest that these intramitochondrial bodies may be related to the so called keratohyaline granules of skin seen in the light microscope. A similar finding has been reported previously from electron micro-1 The transverse lamellae of mitochondria appear in thin sections as two parallel dense lines which enclose a less dense area; they were designated cristae mitochondriales by Palade in 1952 (11). scope studies on experimentally induced squamous metaplasia of mouse cornea (13). MATERIALS AND METIIODSEar epidermis of adult male Swiss mice was made hypcrplastic by painting twice weekly with 5 per cent croton oil in mineral oil for 5 weeks. Specimens of such epidcrmis obtained from four animals were fixed either in buffered I per cent osmium tetroxide at pH 7.2-7.4 07) or in similarly buffered 2 per cent potassium permanganate (6), and embedded in Epon by the method of Luft (7). Thin sections were stained with lead hydroxide (15) and examined in an RCA EMU 3E microscope.

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Section: Discussionmentioning

confidence: 99%

Corpus Intra Cristam: A Dense Body Within Mitochondria of Cells in Hyperplastic Mouse Epidermis

Frei

Sheldon

1961

The Journal of Cell Biology

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“…Observations by Luft and Hechter (1957) point to the possibility that such pore-like interruptions may perhaps be related to tissue anoxia. They add, however, that judgment should be withheld until more data are available.…”

Section: Discussionmentioning

confidence: 99%

An Electron Microscopic Study of the Ductuli Efferentes and Rete Testis of the Guinea Pig

Ladman

Young

1958

The Journal of Cell Biology

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The ductnii efferentes and rete testis of the guinea pig were isolated by micro dissection, fixed in cold buffered osmium tetroxide, and sectioned for examination with the light and electron microscopes.Proximal and distal segments of the duetnii efferentes were identified and their respective cytological organizations characterized. The cytological components of the rete testis are briefly described and figured.Non-ciliated and dliated ceils are found in both segments of the ductuli efferentes. The non-cillated cells have a microvilious border, mitochondria, a Golgi complex, an ubiquitous endoplasmic reticulum, and numerous cytoplasmic vacuoles. The ciliated ceils contain more mitochondria, an endoplasmic reticulum with a relatively sparse distribution, and few, if any, cytoplasmic vacuoles. A regional difference exists in proximal and distal segments based on the distribution, size, number, and eleetron opacity of the cytoplasmic vacuoles. Attention was paid to the disposition of the endoplasmic reticulum and its relation to the system of cytoplasmic vacuoles. These findings are interpreted as suggesting that the continuity of the vacuolar system with elements of the endoplasmic reticulum represents a pathway for transfer of large quantities of fluid, an activity which has long been ascribed to the epithelium of the ductuli efferentes.Periductular capillaries possess pore-like apertures in their endothelia similar to those in other tissues known to engage in fluid transfer.The capacity for reabsorption by the epithelial cells of the ductuli efferentes was first postulated by yon Mifllendorff (1920Mifllendorff ( , 1922. He found that these cells store subcutaneously injected vital dyes such

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“…The second possibility is that the fenestrations were formed under hypoxia. Luft and Hechter (1957) observed fenestrated capillaries in the cow adrenal cortex, if the tissue was fixed 1-2 h after the death of the animal, but if it was perfused with warm serum during that interval, the capillaries were non-fenestrated.…”

Section: Fenestrationsmentioning

confidence: 94%

Microvasculature of human colorectal epithelial tumors

Wuru

Campiche

1982

Virchows Arch. A Path. Anat. and Histol.

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The ultrastructure of the microvasculature in human colorectal adenomas and carcinomas was studied, and compared with that of normal tissue. Blood vessels in adenomas were generally of normal structure, whereas carcinoma vessels displayed a variety of structural alterations. Endothelial proliferation was frequently observed in all the eleven carcinomas examined, which confirms the reports of new vessel formation in experimental malignant tumors. The presence of fenestrations in obviously abnormal endothelium was tentatively attributed to hypoxia, vascular regression or immaturity of the cells, in spite of the fact that normal capillaries in colonic mucosa are fenestrated. Some vessels showed multilayered basement membranes, various types of activated cells containing numerous lysosomal granules, and thickened perivascular tissue. These features may be a vascular response to repeated damage or a manifestation of vascular remodelling. Dilated thin-walled vessels, which were usually found at the periphery of carcinomas, were identified as venules. Small vessels located between the closely apposed carcinoma tubules were not obviously abnormal. They were nevertheless considered to be tumor-induced vessels which had undergone differentiation. The differences in vascular morphology between adenomas and carcinomas were considered to be due to their different growth patterns, growth rates and degrees of maturity.

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An Electron Microscopic Correlation of Structure with Function in the Isolated Perfused Cow Adrenal, Preliminary Observations

Cited by 68 publications

References 10 publications

Corpus Intra Cristam: A Dense Body Within Mitochondria of Cells in Hyperplastic Mouse Epidermis

Corpus Intra Cristam: A Dense Body Within Mitochondria of Cells in Hyperplastic Mouse Epidermis

An Electron Microscopic Study of the Ductuli Efferentes and Rete Testis of the Guinea Pig

Microvasculature of human colorectal epithelial tumors

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