An elicitor from Pyricularia oryzae induces resistance responses in rice: isolation, characterization and physiological properties

Schaffrath, Ulrich; Scheinpflug, H.; Reisener, H. J.

doi:10.1006/pmpp.1995.1023

Cited by 41 publications

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“…It is the case of the glycoprotein purified from the mycelial walls of Pyricularia oryzae, causal agent of the rice blast (20). The previous treatment of rice leaves with the elicitor increased peroxidase, cinamil-alcohol desidrogenase and lipoxigenase activities and reduced disease symptoms in the treated leaves.…”

Section: Resultsmentioning

confidence: 99%

Partial purification of elicitors from Lentinula edodes basidiocarps protecting cucumber seedlings against Colletotrichum lagenarium

Piero¹,

Wulff²,

Pascholati³

2006

Braz. J. Microbiol.

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The shiitake mushroom (Lentinula edodes) has been used in research involving the prevention and the control of human and plant diseases. In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium, and an increase in peroxidase activity in the leaves. With the aim of obtaining molecules of agronomic interest, the crude aqueous extract from L. edodes basidiocarp was fractioned with ammonium sulfate. The fraction corresponding to 40-80% of saturation (p40-80), the most effective in reducing anthracnose on cucumber cotyledons, was submitted to anion exchange chromatography (AEC). After AEC, six protein peaks were obtained and the peak V, containing 34% of the proteins present in p40-80, induced peroxidase increase in the cucumber cotyledons besides reducing anthracnose severity. Separation of peak V proteins by SDS-gel electrophoresis revealed the presence of more than one band in the gel. Thus, a partial purification of elicitors present in the L. edodes basidiocarp was achieved.

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Section: Resultsmentioning

confidence: 99%

Partial purification of elicitors from Lentinula edodes basidiocarps protecting cucumber seedlings against Colletotrichum lagenarium

Piero¹,

Wulff²,

Pascholati³

2006

Braz. J. Microbiol.

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“…Os elicitores bióticos apresentam natureza química variada, como carboidratos (oligossacarídeos) (Ayers et al, 1976ab;Okinaka et al, 1995), glicoproteínas (Basse & Boller, 1992;De Witt & Roseboom, 1980;Farmer & Helgeson, 1987;Parker et al, 1991;Roby et al, 1985;Schaffrath et al, 1995;Wulff, 1997), proteínas (Gustine et al, 1995;Ricci et al, 1989), oligopeptídeos (Hahlbrock et al, 1995;Nürnberger et al, 1994), ácidos graxos (Bostock et al, 1981) e componentes da parede celular vegetal (Davis et al, 1986;Hahn et al, 1981). A variação na natureza química dos elicitores demonstra que não há uma característica estrutural única que possa ser considerada como determinante da atividade elicitora (Smith, 1996).…”

Section: Elicitoresunclassified

“…Elicitores bióticos podem ainda ser classificados em endógenos, como fragmentos da própria planta, liberados pelo ataque de enzimas (Davis et al, 1986;Hahn et al, 1981), ou exógenos como fragmentos do patógeno (Ayers et al, 1976ab;Gustine et al, 1995;Okinaka et al, 1995;Schaffrath et al, 1995 (Schaffrath et al, 1995). Também proteínas de baixa massa molecular denominadas elicitinas, secretadas por Phytophthora spp., são translocadas no vegetal, estimulando necrose e induzindo resistência (Kamoun et al, 1993;Keller et al, 1996;Ricci et al, 1989).…”

Section: Elicitoresunclassified

“…Precipitação etanólica, cetônica ou com sulfato de amônio e seqüência de cromatografias são etapas empregadas durante a purificação de elicitores (Hahn & Albersheim, 1978;Koch et al, 1998;Schaffrath et al, 1995;Wulff & Pascholati, 1999;Labanca, 2002 (Shiraishi et al, 1997;Wulff & Pascholati, 1999).…”

Section: Elicitoresunclassified

“…radicis-lycopersici em plantas de tomate (Benhamou et al, 2001); a glucana da parede celular de P. sojae que promove o acúmulo de fitoalexinas do tipo gliceolina em cotilédones de soja (Abbasi et al, 2001); a glicoproteína purificada a partir do filtrado de cultura de Verticillium dahliae que induz o acúmulo de fitoalexinas em células de algodão (Davis et al, 1998); o elicitor glicoprotéico purificado a partir de P. megasperma f. sp. glycinea que proporciona indução de respostas de defesa como fitoalexinas em células de salsa (Nürnberger et al, 1994) e a glicoproteína purificada a partir do patógeno P. oryzae que induz respostas de defesa em arroz (Schaffrath et al, 1995).…”

Section: Efeito Da Adição De Antibiótico Na Produção De Fitoalexinasunclassified

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Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pepino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum.

Bonaldo¹

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Identification of elicitor‐responsive proteins in rice leaves by a proteomic approach

Liao

Wang

2009

Proteomics

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Experiments were conducted to identify the differentially expressed proteins in rice (Oryza sativa L.) plants after treatment with the glycoprotein elicitor CSB I, purified from ZC(13), a race of the rice blast fungus Magnaporthe grisea. The interactions of two near isogenic lines of rice, C101A51 and CO39, with ZC(13) resulted in completely incompatible and compatible types, respectively. Proteins were extracted from rice leaves at 12 and 24 h after treatment with CSB I. Temporal changes in total proteins were examined using 2-DE. Among more than 900 protein spots reproducibly detected on each gel, 11 were up-regulated, three were down-regulated and seven were newly induced during, at a minimum, one time point. Twenty-one differentially expressed proteins were identified by linear ion trap quadrupole (LTQ)-MS/MS. The identified proteins were classified into six categories based on their putative function reported: (i) defense proteins (PR-10a, PR-5 and putative salt-induced protein), (ii) signal transduction (nucleoside diphosphate kinase and putative profilin), (iii) ROS (Mn-SOD, Cu/Zn-SOD, GST and CAT), (iv) programmed cell death (translationally controlled tumor protein), (v) molecule biosynthesis (putative ribosomal protein S5, putative ribosomal protein L12, putative translational elongation factor Tu and putative chaperonin 21 precursor) and (vi) metabolism (putative fructose-bisphosphate aldolase class-I, putative malate dehydrogenase, cytoplasmic malate dehydrogenase, putative acid phosphatase, putative transketolase1 and gamma hydroxybutyrate dehydrogenase-like protein). All of these proteins (except Cu/Zn-SOD, putative acid phosphatase and translationally controlled tumor protein) were induced faster and to a higher degree in C101A51 than in CO39. These data suggest that the incompatible rice line may possess a more sensitive recognition system that can identify and react to specific chemical, biological or physical triggers in a more efficient manner, thus eliciting an early and fast defense response.

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An elicitor from Pyricularia oryzae induces resistance responses in rice: isolation, characterization and physiological properties

Cited by 41 publications

References 0 publications

Partial purification of elicitors from Lentinula edodes basidiocarps protecting cucumber seedlings against Colletotrichum lagenarium

Partial purification of elicitors from Lentinula edodes basidiocarps protecting cucumber seedlings against Colletotrichum lagenarium

Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pepino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum.

Identification of elicitor‐responsive proteins in rice leaves by a proteomic approach

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