An Emergence of Equine-Like G3P[8] Rotaviruses Associated with Acute Gastroenteritis in Hospitalized Children in Thailand, 2016 −2018

Chaiyaem, Thanakorn; Chanta, Chulapong

doi:10.48022/mbl.2009.09013

Cited by 3 publications

(34 citation statements)

References 45 publications

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“…VP7 and VP4 genes were first amplified using the primer pairs Beg9/End9 or VP7F/VP7R and Con3/Con2, respectively. G-and P-genotypes were then identified using the specific primers for different G genotypes (G1, G2, G3, G4, G9, G12) and P genotypes (P [4], P [6], P [8], P [9], P [10]), respectively. [29][30][31] In addition, a one-step multiplex genotyping strategy was perform to identify RVA strains that were incompletely genotyped.…”

Section: G-and P-genotyping Of Rva By Multiplex Rt-pcr and Dna Sequen...mentioning

confidence: 99%

“…To date, at least 42 G-and 58 P-genotypes are known. G1P [8], G2P [4], G3P [8], G4P [8], G9P [8], and G12P [8] represent the most common and widespread strains causing gastroenteritis in humans. [2][3][4][5][6] The whole genome-based genotyping nomenclature has been developed to assign the genotype constellation of RVA.…”

Section: Introductionmentioning

confidence: 99%

“…G1P [8], G2P [4], G3P [8], G4P [8], G9P [8], and G12P [8] represent the most common and widespread strains causing gastroenteritis in humans. [2][3][4][5][6] The whole genome-based genotyping nomenclature has been developed to assign the genotype constellation of RVA. This system incorporates Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx, where "x" is an integer defining the corresponding genotypes of the VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes.…”

Section: Introductionmentioning

confidence: 99%

“…This system incorporates Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx, where "x" is an integer defining the corresponding genotypes of the VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes. There are three genogroup constellations of human RVA strains: genogroup I with the Wa-like constellation (G1/3/4/9-P [8]-I1-R1-C1-M1-A1-N1-T1-E1-H1), genogroup II with the DS-1-like constellation (G2-P [4]-I2-R2-C2-M2-A2-N2-T2-E2-H2), and genogroup III with the AU-like constellation (G3-P [9]-I3-R3-C3-M3-A3-N3-T3-E3-H3). RVA strains of genogroup I, II, and III have been reported to carry the gene segments of pigs, cattle, and cats/rabbits, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Surveillance of RVA in Chiang Rai province of Thailand has continuously monitored the diversity and distribution of RVA since 2015 and reported the emergence of G9P [8] in 2015-2016 and the equine-like G3P [8] in 2016-2018. 3,4 The purpose of the present study was to investigate the molecular epidemiology and characterization of RVA strains in children hospitalized with acute gastroenteritis in Chiang Rai, Thailand from 2018 to 2020.…”

Section: Introductionmentioning

confidence: 99%

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Predominance of novel DS-1-like G8P[8] rotavirus reassortant strains in children hospitalized with acute gastroenteritis in Thailand

Chan-it

Chanta

Ushijima

2023

Preprint

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Rotavirus A (RVA) is an important cause of acute gastroenteritis (AGE) in children. This study aims to investigate the molecular epidemiology of RVA children hospitalized with AGE in Chiang Rai, Thailand in 2018-2020 by RT-PCR. Of 302 samples, RVA was detected in 11.6% (35 samples): 11.3% (19/168) in 2018-2019 and 11.9% (16/134) in 2019-2020. Surprisingly, G8P[8] was detected as the predominant genotype at 68.4% in 2018-2019 and 81.2% in 2019-2020. In addition, other genotypes were also detected, including G1P[8] (15.8%), G2P[4] (5.3%), G3P[8] (10.5%) in 2018-2019 and G9P[8] (18.8%) in 2019-2020. Analysis of genomic constellation of G8P[8] strains, represented by RVA/Human-wt/ THA/5CR11/2019/G8P[8], revealed a DS-1-like genetic backbone: G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis of the VP7 gene showed that the DS-1-like G8P[8] strains clustered in a distinct sublineage A together with 13 G8P[8] strains reported from Thailand and China, and these sublineage A G8P[8] strains contained unique amino acid substitutions in two positions (A125S and N147D) on the VP7 antigenic epitopes. Homology modeling of the VP7 capsid protein confirmed that these two amino acid changes were located on the surface exposed area of the virion. Phylogenetic trees of the VP1, VP6, NSP2, NSP3, and NSP4 genes have demonstrated that DS-1-like G8P[8] strains in the present study and 51 DS-1-like G8P[8] reference strains published formerly clustered in separate lineages. To the best of our knowledge, this is the first report of the emergence of novel DS-1-like G8P[8] strains that might have evolved genetically through reassortment events with locally or globally circulating genotypes.

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