An Engineered Virus as a Scaffold for Three‐Dimensional Self‐Assembly on the Nanoscale

Blum, Amy Szuchmacher; Soto, Carissa M.; Wilson, Charmaine D.; Brower, Tina; Pollack, Steven K.; Schull, Terence L.; Chatterji, Anju; Lin, Tianwei; Johnson, John E.; Amsinck, Christian; Franzon, Paul D.; Shashidhar, R.; Ratna, Banahalli R.

doi:10.1002/smll.200500021

Cited by 114 publications

(72 citation statements)

References 34 publications

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“…[110,[117][118][119] A conducting network of CPMV particles was created by linking surface-attached Au nanoparticles (2 or 5 nm) through the use of conducting organic molecules. [120] Individual CPMV particles that were decorated with both Au nanoparticles and the conducting organic matrix were placed onto a self-assembled monolayer of undecanethiol on a Au/mica substrate with a dithiolfunctionalized conducting molecule inserted into defect sites.…”

Section: Icosahedral Viral Capsids: Exteriormentioning

confidence: 99%

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Uchida¹,

Klem²,

Allen³

et al. 2007

Advanced Materials

535

518

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Materials scientists increasingly draw inspiration from the study of how biological systems fabricate materials under mild synthetic conditions by using self‐assembled macromolecular templates. Containerlike protein architectures such as viral capsids and ferritin are examples of such biological templates. These protein cages have three distinct interfaces that can be synthetically exploited: the interior, the exterior, and the interface between subunits. The subunits that comprise the building blocks of these structures can be modified both chemically and genetically in order to impart designed functionality to different surfaces of the cage. Therefore, the cages possess a great deal of synthetic flexibility, which allows for the introduction of multifunctionality in a single cage. In addition, hierarchical assembly of the functionalized cages paves the way for development of a new class of materials with a wide range of applications from electronics to biomedicine.

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Section: Icosahedral Viral Capsids: Exteriormentioning

confidence: 99%

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Uchida¹,

Klem²,

Allen³

et al. 2007

Advanced Materials

535

518

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“…Recently, the accurate positioning of NPs on designed sites was demonstrated for the icosahedral CPMV virus [74,75]. Wild-type CPMV was genetically engineered to present cysteine residues at selected positions on the outer surface.…”

Section: Virusesmentioning

confidence: 99%

“…The DM mutants were specifically decorated using 2 nm-sized gold NPs that self-positioned at potentially all the 120 cysteine sites (Figure 3.4G). For the EF and DM viruses, the NP-positioning accuracy could be probed by bridging them with rigid dithiolated conjugated molecules and subsequently observing the hybrid nanostructures by scanning tunneling microscopy [75]. In the absence of bridging molecules, or when the molecular length was smaller than the inter-particle distance, individual NPs could be distinguished on the surface of CPMV due to the increased conductance at the metallic NP sites.…”

Section: Virusesmentioning

confidence: 99%

Synthesis and Assembly of Nanoparticles and Nanostructures Using Bio‐Derived Templates

Dujardin

Mann

2007

Nanobiotechnology II

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The emergence of complexity is a defining feature of our planet. Moreover, complexity is ubiquitous. It is observed in inanimate and animate matter, social systems and communication protocols, and in diverse environmental networks. In each case, these systems exhibit at a fundamental level organizational properties that emerge without the intervention of any distinct organizing agent. This notion provides a formidable challenge for scientists attempting to decipher the general and specific processes responsible for complexity, and is a source of deep inspiration for those who attempt to shape and organize matter through synthetic procedures [1]. Over the past few decades, it has been realized that morphological, structural and functional complexity is often a defining characteristic of biomineralization, which generally involves temporally and spatially dependent interactions between assembling organic scaffolds and inorganic minerals [2]. Fortunately, the overwhelming variety found in biomineralization (and Nature in general) does not necessarily arise from a cumulative complexity involving excessive complication and layering of increasing numbers of components and networks, but rather from an elegant complexity where a limited number of principles and building blocks are combined in a multitude of ways to produce adaptive and evolutive structures with precise functions. This second paradigm offers hope that complexity can also be achieved in synthetic materials if the key concepts and processes can be distilled from the appropriate biological archetypes. For complex nanostructures, a good starting point is to develop experimental approaches based on principles of biomineralization, such as templated nucleation, growth and directed self-assembly. Thus, in this chapter we illustrate how unraveling the specific interactions between bio-derived templates and inorganic materials not only yields a better understanding of natural hybrid materials but also inspires new methods for developing the potential of biological molecules, superstructures and organisms as self-assembling agents for materials fabrication. 39 Nanobiotechnology II. Edited by Chad A. Mirkin and Christof M. Niemeyer

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“…The biotin units on the upper exposed surface of the immobilized CPMV then serve as capture sites for avidin-derivatized quantum dots [167]. Similar conjugation chemistry has been used to produce virus-carbon nanotube [169] and virus-metal nanoparticle complexes [170], which have potential as biological sensors. For example, networks of gold nanoparticles and bacteriophage have recently been engineered to preserve the cell-targeting and internalization properties mediated by the specific peptide display [171].…”

Section: Bioimagingmentioning

confidence: 99%

Bionanoparticles and their Biomedical Applications

Lee

Barnhill

Wang

2003

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction BNPs Genetic and Chemical Alterations of BNPs Chemical Modifications Conventional Bioconjugation Methods for Selective Modifications “Click Chemistry” for Bioconjugation of BNPs New Developments in Tyrosine Modification Genetic Alterations Heterologous Peptide Insertions NAA Substitutions Protein Expression Systems BNPs in Therapeutics Cell Targeting Gene Delivery Bioimaging Drug Encapsulation and Release Immune Response Vaccine Development Immune Modulation Future Directions Acknowledgments

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An Engineered Virus as a Scaffold for Three‐Dimensional Self‐Assembly on the Nanoscale

Cited by 114 publications

References 34 publications

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

Synthesis and Assembly of Nanoparticles and Nanostructures Using Bio‐Derived Templates

Bionanoparticles and their Biomedical Applications

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