An environment for three-dimensional shaded perspective display of cell components

Menhardt, W.; Lockhausen, J.; Dallas, William J.; Kristen, U.

doi:10.1016/0739-6260(86)90027-4

Cited by 11 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first consists of staking all the contour-plots of the boundary structures identified in each section (Macagno et al, 1973;Maziotta and Hamilton, 1977;Peachey et al, 1974). In the second approach, each contour is joined to the next by triangular tiles to form polygon surfaces (Baba et al, 1986;Menhardt et al, 1986). The color-range method we developed using serial sections does not depend on the outlining of structure surfaces, but on the interpretation of significant gray level values in interaction with the computer (Geraud et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Computer reconstruction of nucleolar architecture by interactive three‐dimensional color display

Géraud

Soyer

Hernandez‐Verdun

1991

J. Elec. Microsc. Tech.

View full text Add to dashboard Cite

The first complete three dimensional ultrastructural reconstruction of pancreatic cell nucleoli, was done using EM and computer 3D-assisted reconstruction of serial sections with interactive 3D back-to-front and color display methods based on voxel representation. The purpose of the study was to depict the architecture of the nucleolar components. We obtained information about the location of the nucleolus within the nuclear volume and about the shape and polarity of the 3 main nucleolar territories.

show abstract

Section: Discussionmentioning

confidence: 99%

Computer reconstruction of nucleolar architecture by interactive three‐dimensional color display

Géraud

Soyer

Hernandez‐Verdun

1991

J. Elec. Microsc. Tech.

View full text Add to dashboard Cite

show abstract

“…Following digitization, a contrast enhancement method, known as histogram stretching, was applied to increase the visual image quality. Pixel intensity values corresponding to tissues without interest were mapped to the limits of the grey scale and in the same time grey level values corresponding to the tissue that is currently under investigation, were stretched over the entire grey scale (8,10,11). Proper alignment between slices was essential for accurate reconstruction in three dimensions.…”

Section: Methodsmentioning

confidence: 99%

“…Various techniques and modalities can be used to acquire these sections. The most usually applied method is the physical sectioning by using a microtome (6)(7)(8)(9), and the sections thus taken are subsequently imaged through an optical or electron microscope. A video camera connected to a computer or a photographic camera in conjunction to an image scanner may be used to digitize the images.…”

mentioning

confidence: 99%

Computerized reconstruction of TEM examined pulpal blood vessels and nerves

Lyroudia

Palakidis

Manthos

et al. 1995

Dental Traumatology

View full text Add to dashboard Cite

Five healthy premolars were used for transmission electron microscopy examination and three-dimensional (3D) reconstruction of pulpal blood vessels and their surrounding nerves. Serial Epon thin sections were taken from specimens. After a series of preprocessing steps that included digitization, contrast enhancement, slice alignment, segmentation and interpolation, three dimensional surface representation was performed using the triangulation method. The findings showed that vessels were usually accompanied by a number of myelinated and unmyelinated nerves. The nerves shared a more or less common course with the vessels. Small arteries presented greater number of nerves in their vicinity, compared to small veins. In the sections examined, no direct contact between the nerve trunks and the wall of the vessels was found; the closest distance measured was approximately 67 microns. The common course of vessels and nerves suggested autonomic innervation on part of the myelinated axons.

show abstract

“…The same method cannot be used for electron microscopic samples, because the marks will be too large, thereby destroying important structures. Manual correction methods have been proposed for such samples (Menhardt et al 1986). We will show that the topological-graph representation can be used to compute RST transformations automatically with no fiduciary marks for a complete stack of sections.…”

Section: Introductionmentioning

confidence: 99%

Graph-directed modelling from serial sections

Giertsen¹,

Halvorsen

Flood

1990

The Visual Computer

View full text Add to dashboard Cite

We propose a general 3D-surface reconstruction technique for objects defined in terms of contours in a stack of serial sections. It is built around a data structure, in which the topology of the objects is represented as a set of disconnected graphs. The basic idea is to let the topological information direct the reconstruction process. There are several advantages with this approach. The alignment of the stack of sections is computed automatically. This operation does not depend on artificial reference points, such as fiduciary marks introduced by laser-beam penetration of a biological sample prior to sectioning. Furthermore, the unknown surfaces between successive sections can be created automatically, even though the sections contain a large number of arbitrarily shaped contours that are clustered or perhaps partly overlapped. If present, 3D irregularities in the surface model can be reduced in a controlled manner.

show abstract

An environment for three-dimensional shaded perspective display of cell components

Cited by 11 publications

References 6 publications

Computer reconstruction of nucleolar architecture by interactive three‐dimensional color display

Computer reconstruction of nucleolar architecture by interactive three‐dimensional color display

Computerized reconstruction of TEM examined pulpal blood vessels and nerves

Graph-directed modelling from serial sections

Contact Info

Product

Resources

About