An enzymatic assay for DNA in epoxy-tissue-sections by light microscope-autoradiography

DNA Synthesis in the Tracheae of Aging Mice by Light and Electron Microscopic Radioautography.

“…However, the DNA synthesis of other cells at 1 week was higher than that of on the third day after birth, then fell progressively from 1 month after birth on. The findings correlate well with the reports by several authors [25,41]. Labeled fibroblasts and smooth muscle cells were seen in the rat tracheal rings [25].…”

“…The findings correlate well with the reports by several authors [25,41]. Labeled fibroblasts and smooth muscle cells were seen in the rat tracheal rings [25]. However, the mitotic activity of fibroblasts adult mammals under steady state conditions is very low in most locations.…”

“…We wonder whether the ciliated cell is really a nonproliferating cell throughout animal life from embryo to senescence during aging. Moreover, only a few studies are available on the other cell types such as chondrocytes, fibroblasts and smooth muscle cells [25,49].…”

Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues.

“…Early assays also exploited the TdT reaction in development of procedures for in situ detection of DNA molecules. By means of tritiated dATP and autoradiography, calf thymus TdT was shown to bind deoxynucleotide monophosphate to the free terminal 3'-OH group of denatured DNA (Modak and Bollum, 1970), even after fixation and embedding of the tissue in plastic (Modak et al, 1973). Adapting this approach for use at the ultrastructural level, Fakan and Modak (1973) observed specific labeling, principally of interphase nuclei, nucleolar regions, mitotic chromosomes, and mitochondria.…”

DNA Labeling at Electron Microscopy

“…While playing a crucial role in the evolution and adaptation of the vertebrate immune system [19][20][21][22][23], this reaction is commonly used in vitro to label the 3' ends of double-or single-stranded DNA with labeled nucleotides and to add homopolymer tails to DNA fragments [24][25][26]. By means of tritiated dATP and autoradiography, calf thymus TdT was shown to bind deoxynucleotide monophosphate to the free terminal 3'-OH group of denatured DNA [27], even after fixation and embedding of the biological material in plastic [28]. Adapting this approach for use at the ultrastructural level, Fakan and Modak [29] observed specific labeling, principally of interphase nuclei, nucleolar regions, mitotic chromosomes, and mitochondria.…”