An enzyme histochemical study of the sinuses of reactive lymph nodes

Crocker, J; Williams, Mary C.

doi:10.1002/path.1711420108

Cited by 11 publications

(4 citation statements)

References 14 publications

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“…As schematically summarized as a model in Figure 7, LYVE-1 immunolocalization is different between inner and outer endothelial cells of subcapsular sinuses, probably reflecting the different functions for the transportation of HA and also the adhesion or migration of CD44-positive lymphocytes [8–10]. Such different features between inner and outer endothelial cells were already reported by another histochemical approach for hydrolytic enzymes [6]. …”

Section: Discussionmentioning

confidence: 77%

Histological Study and LYVE-1 Immunolocalization of Mouse Mesenteric Lymph Nodes with "In Vivo Cryotechnique"

Bai

Terada

et al. 2011

Acta Histochem. Cytochem

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The “in vivo cryotechnique” (IVCT) is a powerful tool to directly freeze living animal organs in order to maintain biological components in frozen tissues, reflecting their native states. In this study, mesenteric lymph nodes of living mice were directly frozen with IVCT, and we did morphological studies and immunohistochemical analyses on a hyaluronic acid receptor, LYVE-1. In lymph nodes, widely open lymphatic sinuses were observed, and many lymphocytes adhered to inner endothelial cells along subcapsular sinuses. The LYVE-1 was clearly immunolocalized at inner endothelial cells of subcapsular sinuses, as well as those of medullary sinuses. Conventional pre-embedding electron microscopy also showed LYVE-1 immunolocalization along both the apical and basal sides of cell membranes of inner endothelial cells. By triple-immunostaining for LYVE-1, smooth muscle actin, and type IV collagen, the LYVE-1 was immunolocalized only in the inner endothelial cells, but not in outer ones which were surrounded by collagen matrix and smooth muscle cells. Thus, the functional morphology of lymph nodes in vivo was demonstrated and LYVE-1 immunolocalization in inner endothelial cells of subcapsular sinuses suggests hyaluronic acid incorporation into lymph node parenchyma.

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Section: Discussionmentioning

confidence: 77%

Histological Study and LYVE-1 Immunolocalization of Mouse Mesenteric Lymph Nodes with "In Vivo Cryotechnique"

Bai

Terada

et al. 2011

Acta Histochem. Cytochem

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“…The ultrastructure and the enzyme profile of sinus lining cells varies in different regions. Phagocytic activity, reflected by a-naphthyl acetate, acid phosphatase, and P-glucuronidase (Crocker and Williams, 1984). and by cathepsin-B and -D immunoreactivity, is greater in the deep cortex and medulla than in the subcapsular region.…”

Section: Discussionmentioning

confidence: 85%

“…The phagocytic capacity of cells that make up lymphoid sinuses increases from sinus lining cells to sinus reticular cells and macrophages (Crocker and Williams, 1984;Forkert et al, 1977). The ultrastructure and the enzyme profile of sinus lining cells varies in different regions.…”

Section: Discussionmentioning

confidence: 99%

“…Accessorylstromal cells, including vascular endothelial, lymphoendothelial, follicular dendritic (FDC), interdigitating reticular (IDC), and fibroblastic reticular cells (FRC), are an integral part of the lymphoid system and play an important role in antigen and lymphocyte traffic, lymphocyte activation, and differentiation. Sinus lining cells act as "front-line" filters with phagocytic capacity, particularly in antigenic overload (Crocker and Williams, 1984). FDC with antigen-antibody complexes coupled on their surface by complement and immunoglobulin Fc receptors are crucial in B-cell activation and differentiation (Thorbecke et al, 1994;Lindhout et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistological detection of gap junctions in human lymphoid tissue: connexin43 in follicular dendritic and lymphoendothelial cells.

Krenács

Rosendaal

1995

J Histochem Cytochem.

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We investigated the expression of gap junction connexins26, -32, and -43 in normal, reactive, and diseased human lymphoid tissue with single and double immunolabeling and confocal laser scanning microscopy. In all tissues, connexin43 positivity was detected in follicular dendritic cells positive for CD21 and CD35 antigens, around lymphoendothelial cells moderately positive for Factor VIII, CD31 and cathepsin-D antigens; and somewhat in vascular endothelia including high endothelial venules strongly positive for Factor VIII and CD31 antigens. The ultrastructural hallmark of gap junctions, pentalaminar structures with appropriate spacing, was found in follicular dendritic cell processes. Connexin43 was also detected between smooth muscle and stromal cells of the gut, in capsular fibroblasts, and in tonsil epithelium. Neither connexin32 nor -26 was revealed, except for connexin26 in the tonsil epithelium. In follicular dendritic cells, connexin43 co-localized closely with the desmosomal proteins desmoplakin and desmoglein, suggesting that cell adherence has a role in gap junction formation. Most connexin43 was observed in sinus lining cells of lymph nodes involved in malignancies and in follicular dendritic cells in the light zone of germinal centers where maturing but still proliferating lymphocytes are situated. In the light of their distribution, gap junctions may play a part in regulating the growth of germinal centers and in integrating activating or controlling signals in follicular dendritic and sinus lining cell networks. Because connexin43 is the connexin of stromal cells, finding it in follicular dendritic cells in consistent with the proposal that these cells originate from resident stromal cells.

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Immunohistochemical demonstration of cathepsin B in the macrophages of benign and malignant lymphoid tissues

Crocker

Burnett

Jones

1984

The Journal of Pathology

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Cathepsin B has been demonstrated by immunohistochemical means in the macrophages of palatine tonsils, reactive lymph nodes and in specimens of Hodgkin's disease (HD) and non-Hodgkin's lymphomas (NHL). Two cases of genuine histiocytic lymphoma showed strong staining for the enzyme in most cells. In Hodgkin's disease, many Reed-Sternberg cells and Hodgkin cells were positive. Branching and 'tingible body' macrophages (histiocytic reticulum cells, HRCs) were strongly positive in all of the specimens. In reactive lymph nodes, the sinus-lining cells and intrasinusoidal macrophages were positive for cathepsin B. True dendritic reticulum cells (DRCs) appeared to be negative. Unlike muramidase (lysozyme), cathepsin B is not seen in neutrophil polymorph leucocytes.

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An enzyme histochemical study of the sinuses of reactive lymph nodes

Cited by 11 publications

References 14 publications

Histological Study and LYVE-1 Immunolocalization of Mouse Mesenteric Lymph Nodes with "In Vivo Cryotechnique"

Histological Study and LYVE-1 Immunolocalization of Mouse Mesenteric Lymph Nodes with "In Vivo Cryotechnique"

Immunohistological detection of gap junctions in human lymphoid tissue: connexin43 in follicular dendritic and lymphoendothelial cells.

Immunohistochemical demonstration of cathepsin B in the macrophages of benign and malignant lymphoid tissues

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