An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

Baldani, Cristiane Divan; Machado, Rosangela Zacarias; Botteon, Paulo de Tarso Landgraf; Takakura, F. S.; Massard, Carlos Luiz

doi:10.1590/s0103-84782004000500031

Cited by 18 publications

(28 citation statements)

References 12 publications

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“…Also, an ELISA test was performed as reported elsewhere (BALDANI et al, 2004) with an antigen concentration of 10 µg.mL -1 and a serum dilution of 1:100. The immunological activity of each serum was calculated by determining the sample-to-positive serum ratio (S/P), considering positive and negative sera as reference.…”

Section: Theileria Equi Serologymentioning

confidence: 99%

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

Salvagni

Dagnone

Gomes³

et al. 2010

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is a zoonotic disease caused by gram-negative and intracellular obligatory bacterial organisms. Equine Granulocytic Anaplasmosis - EGA (formerly Equine Granulocytic Ehrlichiosis, EGE) is a seasonal disease, normally self-limited in horses. There are few reports in Brazil about this ehrlichial agent, as well as its natural vectors. Nowadays, veterinarians are considering the suspicion of EGA in horses with suggestive symptoms of ehrlichiosis and which do not respond to piroplasmosis treatment. The aim of the present study was to identify horses exposed to the agent A. phagocytophilum by serological and molecular techniques. Twenty equine blood and serum samples from the central West region of Brazil were evaluated by microscopic examination of buffy coat smear, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR). Additionally, the serodiagnosis of Theileria equi by IFA and ELISA were carried out, as well as molecular diagnosis by nPCR. Thirteen (65%) serum samples were positive for A. phagocytophilum by ELISA, but none of them were positive by buffy-coat smear examination or nPCR. Antibodies IgG anti-T. equi were detected in 18 (90%) and 17 (85%) horses by IFA and ELISA, respectively and the agent was detected in 9 (45%) animals by nPCR. Our data may be considered as important information to understanding the occurrence of EGA and equine piroplasmosis in central West Brazil.

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Section: Theileria Equi Serologymentioning

confidence: 99%

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

Salvagni

Dagnone

Gomes³

et al. 2010

Rev. Bras. Parasitol. Vet.

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“…Serological studies concerning infection by B. caballi and T. equi have shown that these two agents are widely distributed in Brazil, with herd prevalence ranging from 1.4 to 100% (BARBOSA et al, 1995;BITTENCOURT et al, 1997;KERBER et al, 1999;RIBEIRO et al, 1999;XUAN et al, 2001;BOTTEON et al, 2002;BALDANI et al, 2004;2007;COSTA PEREIRA et al, 2005;HEIM et al, 2007). These values have been shown to be affected by climatic conditions and farm management.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of equine Piroplasmosis and its association with tick infestation in the State of São Paulo, Brazil

Kerber¹,

Labruna

Ferreira

et al. 2009

RBPV

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Serum samples were collected from 582 horses from 40 stud farms in the State of São Paulo and tick (Acari: Ixodidae) infestations were evaluated on them. Serum samples were subjected to the complement fixation test (CFT) and a competitive inhibition ELISA (cELISA) for Babesia caballi and Theileria equi. Logistic regression analyses were performed to construct multivariate models that could explain the dependent variable (horses positive for B. caballi or T. equi) as a function of the independent variables (presence or abundance of each one of the tick species found on the farms). A higher overall prevalence of B. caballi (54.1%) than of T. equi (21.6%) was found by the two tests. The ticks Dermacentor nitens Neumann, 1897, Amblyomma cajennense (Fabricius, 1787) and Rhipicephalus (Boophilus) microplus (Canestrini, 1887) were present on horses on 38 (95%), 20 (50%), and 4 (10%) farms, respectively. Infestations by D. nitens were statistically associated with B. caballi-positive horses on the farms by either the CFT or cELISA. Infestations by A. cajennense were statistically associated with T. equi-positive horses on the farms by either CFT or cELISA.Keywords: Babesia caballi, Theileria equi, Brazil, horse, tick. ResumoAmostras de soro sanguineo foram coletadas de 582 equinos de 40 haras no estado de São Paulo, onde as infestações por carrapatos foram avaliadas nos animais. Os soros foram testados por reação de fixação do complemento (RFC) e ELISA competitivo por inibição (cELISA) com antígenos de Babesia caballi e Theileria equi. Análises de regressão logística foram realizadas para construir modelos multivariados que pudessem explicar as variáveis dependentes (equinos positivos para B. caballi ou T. equi) em função de variáveis independentes (presença e abundância de cada uma das espécies de carrapatos encontradas nos equinos dos haras). Em geral, os dois testes sorológicos indicaram uma prevalência maior para B. caballi (54,1%) do que para T. equi (21,6%). Os carrapatos Dermacentor nitens Neumann, 1897, Amblyomma cajennense (Fabricius, 1787) e Rhipicephalus (Boophilus) microplus (Canestrini, 1887) estiveram presentes em equinos de 38 (95%), 20 (50%) e 4 (10%) haras, respectivamente. As infestações por D. nitens estiveram estatisticamente associadas com equinos positivos para B. caballi tanto pela RFC como pelo cELISA. As infestações por A. cajennense estiveram estatisticamente associadas com equinos soropositivos para T. equi, tanto pela RFC como pelo cELISA.Palavras-chave: Babesia caballi, Theileria equi, Brasil, cavalo, carrapato.

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“…Heuchert et al (1999) tested 752 serum samples from the State of São Paulo by IFAT and CFT and reported prevalence rates of 29.6% and 17.6%, respectively. Moreover, serum samples from 90 horses of the same region of the present study were tested by ELISA and the prevalence of B. equi found was 75% (Baldani et al 2004). The frequency of equine piroplasmosis recorded in this study is significantly higher than that recorded by Heuchert et al (1999).…”

Section: Resul Resul Resul Resul Results and Discus Ts And Discus Ts mentioning

confidence: 87%

“…Therefore, the advantages and disadvantages of each test should be considered at the moment of choice of the best test for the diagnosis of equine piroplasmosis. It should be mentioned that the standardization of the ELISA was performed in a previous study, which demonstrated that the specificity and sensitivity of the test using a crude antigenic preparation of B. equi was 100% (Baldani et al 2004), with the advantage of using very low concentration of the antigen (10µg/mL) when compared to those reported by Kumar et al (2003), which varied between 370µg/mL and 500µg/mL. The IFAT, on the other hand, showed cross-reactions with B. caballi reference sera, although titers were not very high (≤1:80).…”

Section: Resul Resul Resul Resul Results and Discus Ts And Discus Ts mentioning

confidence: 99%

“…Enzyme-link Enzyme-link Enzyme-link Enzyme-link Enzyme-linked immunosorbent assay (ELISA) ed immunosorbent assay (ELISA) ed immunosorbent assay (ELISA) ed immunosorbent assay (ELISA) ed immunosorbent assay (ELISA) ELISA was performed as described previously (Baldani et al 2004). Ninety-six-well microtitration plates (Nunclon TM Surface; Nunc, Denmark) were coated overnight at 4ºC with 100µL (10µg/mL) of crude B. equi antigen diluted in a sodium bicarbonate-carbonated 0.05M buffer (pH 9.6).…”

Section: Indirect Fluorescent Antibody Test (If Indirect Fluorescent mentioning

confidence: 99%

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Serodiagnosis of Babesia equi in horses submitted to exercise stress

et al. 2007

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An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

Cited by 18 publications

References 12 publications

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

Prevalence of equine Piroplasmosis and its association with tick infestation in the State of São Paulo, Brazil

Serodiagnosis of Babesia equi in horses submitted to exercise stress

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