An Epitope‐Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell–Biomaterial Interactions

Pan, Guoqing; Shinde, Sudhirkumar; Yeung, Sing Yee; Jakštaitė, Miglė; Li, Qianjin; Wingren, Anette Gjörloff; Sellergren, Börje

doi:10.1002/ange.201708635

Cited by 20 publications

(9 citation statements)

References 66 publications

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“…Statistical analysis showed that the live cells on FN-MIP membrane were higher than those on NIP membrane because the FN-MIP membrane could adsorb more FN than NIP membrane, which made it was more suitable for the adhesion and growth of cells. Pan et al [ 42 , 43 ] introduced the cell-adhesive peptide (RGDS) onto a thermo-responsive cell culture substrate. The substrate could be used as a highly efficient novel system.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

Liu

Zhao

et al. 2018

Polymers

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Bovine serum albumin (BSA) molecular-imprinted polysiloxane (MIP) membrane was prepared by sol-gel technology, using silanes as the functional monomers, BSA as the template and CaAlg hydrogel film as the matrix. The stress-strain curves of wet CaAlg membrane and molecular-imprinted polysiloxane membrane were investigated. We evaluate the adsorption and recognition properties of MIP membrane. Results showed that the adsorption capacity of BSA-imprinted polysiloxane for BSA reached 28.83 mg/g, which was 2.18 times the non-imprinted polysiloxane (NIP) membrane. The adsorption rate was higher than that of the protein-imprinted hydrogel. BSA-imprinted polysiloxane membrane could identify the protein template from competitive proteins such as bovine hemoglobin, ovalbumin and bovine γ-globulin. In order to obtain the biomaterial that can promote cell adhesion and proliferation, fibronectin (FN)-imprinted polysiloxane (FN-MIP) membrane was obtained by using fibronectin as the template, silanes as functional monomers, and CaAlg hydrogel membrane as the substrate or matrix. The FN-MIP adsorbed more FN than NIP. The FN-imprinted polysiloxane membrane was applied to culture mouse fibroblast cells (L929) and the results proved that the FN-MIP had a better effect on cell adhesion than NIP.

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Section: Resultsmentioning

confidence: 99%

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

Liu

Zhao

et al. 2018

Polymers

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“…[189] Another good example of the use of MI was presented by Pan et al In this work, epitope imprinting was applied to create a biointerface with dynamic bioactivity, allowing easy harvesting of cell sheets without interfering with cell viability. [195] The authors designed a carboxyl-rich peptide (DDDGGDDD) which was used to imprint a benzamidine-based polymeric surface. This could then be used to capture another peptide, DDDGGDDDSSSSSRGDS, comprising three components.…”

Section: Next-generation Synthetic Platforms With Abiotic Affinitymentioning

confidence: 99%

“…This caused the release of attached fibroblasts, with over 90% of cells detaching after 12 h. Importantly, detached cells could then be cultured again on a new Petri dish, thus implying that the molecule-exchange-induced cell release occurs in a noninvasive manner. [195] While they may be few, these constitute promising first steps to take advantage of synthetic materials and technologies to produce biomaterials with selective recognition properties. Many of the pitfalls that have been pointed out for other platforms Figure 5.…”

Section: Next-generation Synthetic Platforms With Abiotic Affinitymentioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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“…In a more recent paper by the same authors, microcontact printing was used to imprint a surface with RGD. The authors showed the ability of the imprinted surface to reversibly bind and re-bind the peptide, effectively creating a platform with tunable cell-adhesion properties [94].…”

Section: Molecular Imprinting To Introduce Chemical Cues Inside the Smentioning

confidence: 99%

Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

2021

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Tissue Engineering (TE) represents a promising solution to fabricate engineered constructs able to restore tissue damage after implantation. In the classic TE approach, biomaterials are used alongside growth factors to create a scaffolding structure that supports cells during the construct maturation. A current challenge in TE is the creation of engineered constructs able to mimic the complex microenvironment found in the natural tissue, so as to promote and guide cell migration, proliferation, and differentiation. In this context, the introduction inside the scaffold of molecularly imprinted polymers (MIPs)—synthetic receptors able to reversibly bind to biomolecules—holds great promise to enhance the scaffold-cell interaction. In this review, we analyze the main strategies that have been used for MIP design and fabrication with a particular focus on biomedical research. Furthermore, to highlight the potential of MIPs for scaffold-based TE, we present recent examples on how MIPs have been used in TE to introduce biophysical cues as well as for drug delivery and sequestering.

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An Epitope‐Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell–Biomaterial Interactions

Cited by 20 publications

References 66 publications

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

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