An essential oil and its major constituent isointermedeol induce apoptosis by increased expression of mitochondrial cytochrome c and apical death receptors in human leukaemia HL-60 cells

Kumar, Ajay; Malik, Fayaz; Bhushan, Shashi; Sethi, Vijay; Shahi, A. K.; Kaur, Jagdeep; Taneja, Subhash C.; Qazi, Ghulam N.; Singh, Jitender

doi:10.1016/j.cbi.2007.10.003

Cited by 95 publications

(44 citation statements)

References 33 publications

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“…The loss of lipid asymmetry and exposure of phosphatidylserine can be detected using fluorochrome-conjugated annexin V (a Ca 2+ -dependent phospholipid binding protein), but because phosphatidylserine exposure also occurs during necrosis, simultaneous staining for membrane permeability (eg, with PI) enables the 2 states to be distinguished. 24,28 So the flow cytometric analysis of annexin V-/PI-labeled cells was done. Healthy cells were negative for both PI and annexin V. Apoptotic cells were PI negative and annexin V positive.…”

Section: Discussionmentioning

confidence: 99%

Sphaeranthus indicus Induces Apoptosis Through Mitochondrial-Dependent Pathway in HL-60 Cells and Exerts Cytotoxic Potential on Several Human Cancer Cell Lines

Nahata

Suri

et al. 2012

Integr Cancer Ther

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Purpose. The study was designed to screen Sphaeranthus indicus, Ganoderma lucidum, and Urtica dioica for their anticancer activity against human cancer cell lines. Phytochemical screening of active extracts was also planned. Methods. Petroleum ether, ethanolic, and aqueous extracts of S indicus Linn, G lucidum P Karst, and U dioica Linn were subjected to cytotoxicity studies using 7 different cancer cell lines. Potent cytotoxicity was noted in petroleum ether extract of S indicus (SIP), which inhibited proliferation of various cancer cell lines. Growth inhibition was determined by sulforhodamine B assay. Two biochemical markers, namely β-sitosterol and 7-hydroxyfrullanolide were isolated and characterized using high-performance thin layer chromatography, melting point, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and mass analysis. Cytotoxicity of isolated β-sitosterol and 7-hydroxyfrullanolide were also determined. The IC 50 of SIP was calculated in the HL-60 cells and was found to be 53 µg/mL. Furthermore, SIP induced apoptosis in human leukemia HL-60 cells as measured by several biological end points. Cell cycle analysis and change in mitochondrial membrane potential was quantified by flow cytometry. Subsequently, using annexin V/PI assay, proportion of cells actively undergoing apoptosis was determined. Changes in DNA were observed by DNA ladder assay. Results. SIP induced apoptotic bodies formation, induced DNA laddering, enhanced annexin-V-FITC binding of the cells, increased sub-G 0 DNA fraction, and induced loss of mitochondrial membrane potential (ΔΨm) in HL-60 cells. SIP also elevated the caspase 3 and caspase 9 levels in the HL-60 cells, which clearly indicates the involvement of the intrinsic proteins in inducing apoptosis. Discussion. All the above parameters revealed that SIP induced apoptosis through the mitochondrial-dependent pathway in HL-60 cells. The criterion for anticancer activity in cytotoxicity assay was ≥70% growth inhibition at 100 µg/mL against at least 4 cell lines. As G lucidum and U dioica did not exhibit appreciable inhibitory activity against human cancer cell lines (less than 50%), they were not included in the study thereafter. The results established that SIP has apoptosis-inducing effect against HL-60 cells in vitro and is a promising candidate for further anticancer study. β-Sitosterol and 7-hydroxyfrullanolide can be considered to be potent anticancer compounds isolated from SIP on the basis of present studies.

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Section: Discussionmentioning

confidence: 99%

Sphaeranthus indicus Induces Apoptosis Through Mitochondrial-Dependent Pathway in HL-60 Cells and Exerts Cytotoxic Potential on Several Human Cancer Cell Lines

Nahata

Suri

et al. 2012

Integr Cancer Ther

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“…As in bacterial cells, the cell membrane is one of the sites of action where essential oils and essential oil components were shown to cause permeabilization and depolarisation and to reduce the activity of membrane-associated enzymes [82]. In addition, an interaction with cellular metabolism [82,83] and an induction of apoptosis have been demonstrated for essential oils and oil components [84][85][86].…”

Section: Cytotoxicity Of Essential Oils In Vitromentioning

confidence: 99%

Essential Oils of Aromatic Plants with Antibacterial, Antifungal, Antiviral, and Cytotoxic Properties – an Overview

Reichling¹,

Schnitzler

Suschke³

et al. 2009

Complement Med Res

353

266

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The abundant use of anti-infective agents resulted in the emergence of drug-resistant bacteria, fungi, and viruses. To overcome the increasing resistance of pathogenic microbes, a variety of medicinal plants have been screened worldwide for their antimicrobial properties. The aim is to find new, effective antimicrobial agents with novel modes of actions. Essential oils derived from aromatic medicinal plants have been reported to exhibit exceptionally good antimicrobial effects against bacteria, yeasts, filamentous fungi, and viruses. The progress of this expanding scientific field will be documented by the most important results published in the last decade.

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“…The genus Cymbopogon comprises about 140 species of which most are aromatic and yield an essential oil upon the steam distillation of their aerial parts (Khanuja et al, 2005). Semi-chemical properties of the Cymbopogon essential oils have been promising in integrated pest management programme since this property may lead to development of alternatives to synthetic chemical pesticides (Kumar et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antimicrobial activities of Cymbopogon schoenanthus

Ali¹,

Ibrahim²,

Ahmed³

et al. 2017

Afr. J. Microbiol. Res.

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Medicinal plants have been widely used in folk medicine. They are considered today as an interesting source of new drug due to their bioactive components. The genus, Cymbopogon (Poaceae) seems to be the most promising source of several bioactive compound. This study aimed at evaluating the antimicrobial activities of Cymbopogon schoenanthus on several pathogenic bacteria, fungi and virus. Antibacterial and antifungal activities were evaluated using the agar well diffusion methods. The MIC was determined by micro-broth dilution methods. The cytotoxicity of C. schoenanthus extracts was evaluated using MTT assay. The results showed that methanol extract of C. schoenanthus exhibit an antibacterial effect on several Gram-positive and negative bacteria. All extracts tested (aqueous extract, methanol extract and ethyl acetate extract) were found to have an antiviral effect on HSV1; whereas, no antifungal effect was detected on both Candida albicans and Aspergillus niger.

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An essential oil and its major constituent isointermedeol induce apoptosis by increased expression of mitochondrial cytochrome c and apical death receptors in human leukaemia HL-60 cells

Cited by 95 publications

References 33 publications

Sphaeranthus indicus Induces Apoptosis Through Mitochondrial-Dependent Pathway in HL-60 Cells and Exerts Cytotoxic Potential on Several Human Cancer Cell Lines

Sphaeranthus indicus Induces Apoptosis Through Mitochondrial-Dependent Pathway in HL-60 Cells and Exerts Cytotoxic Potential on Several Human Cancer Cell Lines

Essential Oils of Aromatic Plants with Antibacterial, Antifungal, Antiviral, and Cytotoxic Properties – an Overview

Evaluation of the antimicrobial activities of Cymbopogon schoenanthus

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