An esterase from Penicillium decumbens P6 involved in lignite depolymerization

Yi, Yang; Yang, Jinshui; Li, Baozhen; Wang, En Tao; Yuan, Hongli

doi:10.1016/j.fuel.2017.11.035

Cited by 24 publications

(5 citation statements)

References 31 publications

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“…Later, content of HAs and water-soluble HS was shown to increase markedly while molecular mass of HA decreased in lignite depolymerized by Penicillium decumbens strain P6 (Dong et al 2006). Depolymerization is believed to be a consequence of an esterase (Yang et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Bacterial degradation of coal discard and geologically weathered coal

Olawale

Edeki

Cowan

2020

Int J Coal Sci Technol

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The biodegradation of coal discard is being intensively studied in South Africa in an effort to develop passive methods for the successful revegetation and rehabilitation of waste dumps, to mitigate pollution, and facilitate mine closure. Bacteria were isolated from slurries of coal tailings and diesel-contaminated soil, screened for coal biodegradation competence, characterized, and the colonization and degradation of coal discard and geologically weathered coal investigated using individual isolates and consortia. Ten novel coal-degrading bacterial strains were isolated and characterized, the gene sequences deposited with GenBank, and the (wild-type) strains deposited at Microbial Culture Collection, India. The results from the present work show that bituminous coal discard and geologically weathered coal is used by these isolates as carbon and energy source. Isolated strains and consortia colonized and degraded both coal substrates. Growth rate of the isolates is faster and stationery phase achieved sooner in minimal medium containing geologically weathered coal. This observation suggests that the oxygen-rich weathered coal is a more friable substrate and thus readily colonised and biodegraded. A reduction in mass of substrate is demonstrated for both individual isolates and consortia. The changes in pH and associated media colouration occurred concomitant with formation of humic acid-like (HS) and fulvic acid-like substances (FS) which is confirmed following analysis of these products by FT-IR spectroscopy. It is concluded that preferential metabolism of alkanes from the coal substrates provided the carbon and energy for bacterial growth and transformation of the substrates to HS and FS. Keywords Bacteria Á Biodegradation Á Coal discard Á Humic acid Á Fulvic acid Á FT-IR Á Geologically weathered coal Abbreviations CYP Cytochrome P450 DPA Diphenylamine ECCN EBRU culture collection number FT-IR Fourier transform infrared spectroscopy FA Fulvic acid FS Fulvic acid-like substances HA Humic acid HS Humic acid-like substances LAC Laccase PAH Poly aromatic hydrocarbon Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 99%

Bacterial degradation of coal discard and geologically weathered coal

Olawale

Edeki

Cowan

2020

Int J Coal Sci Technol

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“…Cultures of Penicillium decumbens P6 were also shown to depolymerize lignite and early work suggested that both peroxidases and esterases were involved [63]. Later studies confirmed, albeit tentatively, that an esterase activity that had been partially purified using ammonium sulfate precipitation, anion exchange and gel filtration chromatography was able to convert lignite to low-molecular-mass HA with lower-percentage aromatic carbon but higher-percentage aliphatic carbon [64]. As pointed out by Sudheer et al [65], in their detailed assessment of biological mechanisms for green coal utilization, esterases are not typically activated by mediators and steric hindrance will likely prevent the enzyme from accessing the depths of the coal macromolecule limiting its hydrolytic action.…”

Section: Coal Bio-liquefactionmentioning

confidence: 99%

Elaboration of a Phytoremediation Strategy for Successful and Sustainable Rehabilitation of Disturbed and Degraded Land

et al. 2022

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Humans are dependent upon soil which supplies food, fuel, chemicals, medicine, sequesters pollutants, purifies and conveys water, and supports the built environment. In short, we need soil, but it has little or no need of us. Agriculture, mining, urbanization and other human activities result in temporary land-use and once complete, used and degraded land should be rehabilitated and restored to minimize loss of soil carbon. It is generally accepted that the most effective strategy is phyto-remediation. Typically, phytoremediation involves re-invigoration of soil fertility, physicochemical properties, and its microbiome to facilitate establishment of appropriate climax cover vegetation. A myco-phytoremediation technology called Fungcoal was developed in South Africa to achieve these outcomes for land disturbed by coal mining. Here we outline the contemporary and expanded rationale that underpins Fungcoal, which relies on in situ bio-conversion of carbonaceous waste coal or discard, in order to explore the probable origin of humic substances (HS) and soil organic matter (SOM). To achieve this, microbial processing of low-grade coal and discard, including bio-liquefaction and bio-conversion, is examined in some detail. The significance, origin, structure, and mode of action of coal-derived humics are recounted to emphasize the dynamic equilibrium, that is, humification and the derivation of soil organic matter (SOM). The contribution of plant exudate, extracellular vesicles (EV), extra polymeric substances (EPS), and other small molecules as components of the dynamic equilibrium that sustains SOM is highlighted. Arbuscular mycorrhizal fungi (AMF), saprophytic ectomycorrhizal fungi (EMF), and plant growth promoting rhizobacteria (PGPR) are considered essential microbial biocatalysts that provide mutualistic support to sustain plant growth following soil reclamation and restoration. Finally, we posit that de novo synthesis of SOM is by specialized microbial consortia (or ‘humifiers’) which use molecular components from the root metabolome; and, that combinations of functional biocatalyst act to re-establish and maintain the soil dynamic. It is concluded that a bio-scaffold is necessary for functional phytoremediation including maintenance of the SOM dynamic and overall biogeochemistry of organic carbon in the global ecosystem

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“…However, the bioconversion mechanisms of low-rank coal and lignite indicated solubilization and depolymerization were two main principles for the processing of coal (Fakoussa and Hofrichter 1999). For example, ammonium ions and alkaline substances Hofrichter et al 1997;Yuan et al 2006a;Hölker et al 1999), chelators (Cohen et al 1990;Torzilli and Isbister 1994;Hölker et al 1999), surfactants (Polman et al 1994;Yuan et al 2006a), and hydrolases (Hölker et al 1999;Yang et al 2018) were found to play important roles in the biosolubilization of coal, while ligninolytic enzymes (Hofrichter and Fritsche 1997a), laccase (Pyne Jr et al 1987;Srinivasan et al 1995;Hölker et al 1999;Zavarzina et al 2004;Sekhohola et al 2014;Nsa et al 2022), esterase (Yang et al 2018), lignin peroxidase and manganese peroxidase (Lundell and Hatakka 1994;Steffen et al 2002;Klein et al 2014), laccase and other oxidases (Scott and Lewis 1988;Willmann and Fakoussa 1997;Fakoussa and Frost 1999;Temp et al 1999;Grinhut et al 2007;Dashtban et al 2010;Sekhohola and Cowan 2017) performed the crucial functions in the biodepolymerization of coal. Frequently, multiple solubilising agents are secreted concurrently by microbial organisms for the bioconversion of coal Hofrichter and Fritsche 1997b;Hatakka 1994;Ellouze and Sayadi 2016;Ghani et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Optimization of weathered coal biodegradation by Penicillium aculeatum 13-2-1 and UV-visible spectral characteristics of active degraded products

Ren

Zhang

Wang

et al. 2023

BioRes

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Weathered coal is widely distributed in many provinces in China. It has great potential utilization values as soil ameliorant in sustainable agriculture. To find the optimal condition for the biodegradation of weathered coal and obtain the spectral characteristics of active degraded products of coal, the bioactivation of humic acid in weathered coal is essential. In this study, a fungal strain (Penicillium aculeatum 13-2-1) capable of degrading weathered coal was isolated from the rhizosphere of Zelkova serrata. The experimental results using classical one factor at a time method showed that weathered coal of 1.0 g, inoculum of 150 µL, and sodium nitrate of 0.10 g in 100 mL broth were the optimal conditions for the biodegradation of coal, respectively. The variance analysis of orthogonal tests illustrated that the three factors had little effects on biodegradation of weathered coal, but the weathered coal significantly negatively affected the contents of soluble humic acids in liquid coal products. Different diluted solutions of liquid coal products for all orthogonal combinations promoted the growth of the seeds of Brassica napus L., especially on radicles. The UV-visible spectra of the liquid coal products for all combinations presented the differential absorbance closely associated with the quantity of sodium nitrate in medium.

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An esterase from Penicillium decumbens P6 involved in lignite depolymerization

Cited by 24 publications

References 31 publications

Bacterial degradation of coal discard and geologically weathered coal

Bacterial degradation of coal discard and geologically weathered coal

Elaboration of a Phytoremediation Strategy for Successful and Sustainable Rehabilitation of Disturbed and Degraded Land

Optimization of weathered coal biodegradation by Penicillium aculeatum 13-2-1 and UV-visible spectral characteristics of active degraded products

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