An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS

Nilsen, Mari Mæland; Meier, Sonnich; Larsen, Bodil Katrine; Andersen, Odd Ketil; Hjelle, Anne

doi:10.1016/j.ecoenv.2011.07.036

Cited by 10 publications

(6 citation statements)

References 45 publications

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“…This potent biomarker was determined to be a zona radiata protein (ZRP), fragment 2 12. In Atlantic cod ( Gadus morhua ), the same proteomic technique was used to identify 2 peaks, which were determined to be fragments of vitellogenin protein and ZRP 15. Those proteins significantly increased in the male liver of Salmo salar to nonylphenol, a well-known estrogen-disrupting compound 29.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, one of the major disadvantages of SELDI-TOF MS is the lack of direct identification of the discriminatory peaks discovered. Recently, direct identification after proteinchip elution was demonstrated 15. This technique was used to identify a biomarker candidate in the plasma of Atlantic cod ( G. morhua ) using a combination of SELDI-TOF MS and on-chip elution, which involved a highly sensitive LC-MS/MS system.…”

Section: Resultsmentioning

confidence: 99%

“…SELDI has been used as a proteomic profiling method, in particular, by researchers conducting clinical/pre-clinical studies in which large number of samples are analyzed and more standard proteomic approaches (such as 2-DE or LC MS/MS) are not suitable due to their lower throughput 14. Recently, proteomic profiling using SELDI-TOF MS has been used for the rapid determination of intact biomarker candidates in fish, especially estrogen disrupting compounds 12,15.…”

Section: Introductionmentioning

confidence: 99%

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Endosulfan-Induced Biomarkers in Japanese Rice Fish (Oryzias latipes) Analyzed by SELDI-TOF-MS

Lee¹,

Choi²,

Mo³

et al. 2013

Int. J. Biol. Sci.

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The objective of this study was to find and validate estrogen-related biomarkers from plasma proteins in Oryzias latipes after exposure to an estrogen disrupting compound, α-endosulfan. The acute toxicity of α-endosulfan on O. latipes after 96 h of exposure was 13.72, 16.18, and 22.18 μg L-1 for the LC10, LC20, and LC50 values, respectively. To confirm estrogenic disturbance by α-endosulfan, the expression level of vitellogenin in the liver of male fishes was measured at the LC10 value, and it was found to be significantly different from the reference group, confirming the estrogenic effect of endosulfan in this concentration range. Proteinchip® array techniques using a weak cation exchange (CM10) and a strong anion exchange proteinchip (Q10) in conjunction with surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) were used to determine plasma proteins of O. latipes differently expressed in response to endosulfan exposure at LC10 and LC20 concentrations. Analysis of protein profiling of the male fish exposed to α-endosulfan detected 48 significantly different protein peaks and the proteins at m/z 2819, 8462, 8860, and 9462 were significantly different (p<0.05). The protein peaks at m/z 2819, 8860, and 9462 were up-regulated and the peak at m/z 8462 was down-regulated. Therefore, these four differentially expressed proteins could be used as biomarkers to rapidly determine a possible risk of endosulfan on aquatic ecosystems, although these are not necessarily produced as a result of endocrine disruption.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endosulfan-Induced Biomarkers in Japanese Rice Fish (Oryzias latipes) Analyzed by SELDI-TOF-MS

Lee¹,

Choi²,

Mo³

et al. 2013

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…A peak with m/z 3267 has previously been found to be strongly up-regulated in plasma of Atlantic cod exposed to the xenoestrogenic compound, 4-nonylphenol [4]. An antibody-assisted SELDI-TOF MS approach indicated that the peak correlated to a fragment of at least one larger protein (i.e.…”

Section: Seldi-tof Ms Elutionmentioning

confidence: 96%

“…A mixture of four known proteins was used to test and optimize the novel identification method, and its practical application was evaluated by attempting to identify a biomarker candidate previously discovered by SELDI-TOF MS in plasma from Atlantic cod (Gadus morhua) [4].…”

Section: Seldi-tof Ms Elutionmentioning

confidence: 99%