An Evaluation of Aerobic Trichloroethene Attenuation Using First-Order Rate Estimation

Sorenson, Kent S.; Peterson, Lance N.; Hinchee, Robert E.; Ely, Roger L.

doi:10.1080/10889860091114293

Cited by 29 publications

(31 citation statements)

References 25 publications

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“…Though the extent of this plume is quite large, it does not appear to be migrating farther from the initial point of contamination. A previous study ruled out abiotic loss as the only reason for this observed attenuation (47). In addition, direct evidence that TCE cometabolism is occurring at the TAN site within the plume of TCE contamination has been reported (M. H. Howard-Jones, W. K. Keener, R. A. Wymore, F. S. Colwell, K. S. Sorenson, and M. E. Watwood, Abstr.…”

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confidence: 98%

Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer

Erwin

Erickson

Delwiche

et al. 2005

Appl Environ Microbiol

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PCR amplification, restriction fragment length polymorphism, and phylogenetic analysis of oxygenase genes were used for the characterization of in situ methane-and ammonia-oxidizing bacteria from free-living and attached communities in the Eastern Snake River Plain aquifer. The following three methane monooxygenase (MMO) PCR primer sets were used: A189-A682, which amplifies an internal region of both the pmoA gene of the MMO particulate form and the amoA gene of ammonia monooxygenase; A189-mb661, which specifically targets the pmoA gene; and mmoXA-mmoXB, which amplifies the mmoX gene of the MMO soluble form (sMMO). Whole-genome amplification (WGA) was used to amplify metagenomic DNA from each community to assess its applicability for generating unbiased metagenomic template DNA. The majority of sequences in each archive were related to oxygenases of type II-like methanotrophs of the genus Methylocystis. A small subset of type I sequences found only in free-living communities possessed oxygenase genes that grouped nearest to Methylobacter and Methylomonas spp. Sequences similar to that of the amoA gene associated with ammoniaoxidizing bacteria (AOB) most closely matched a sequence from the uncultured bacterium BS870 but showed no substantial alignment to known cultured AOB. Based on these functional gene analyses, bacteria related to the type II methanotroph Methylocystis sp. were found to dominate both free-living and attached communities. Metagenomic DNA amplified by WGA showed characteristics similar to those of unamplified samples. Overall, numerous sMMO-like gene sequences that have been previously associated with high rates of trichloroethylene cometabolism were observed in both free-living and attached communities in this basaltic aquifer.

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confidence: 98%

Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer

Erwin

Erickson

Delwiche

et al. 2005

Appl Environ Microbiol

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“…The aquifer resides deep within fractured basalt, making contaminant characterization and ex situ remediation difficult and expensive. TCE concentrations of at least 32,000 g liter Ϫ1 have been measured within the plume that spans an area of approximately 1 by 3 km and extends from 64 m to greater than 155 m in depth (74).…”

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confidence: 99%

Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture

Macbeth

Cummings

Spring

et al. 2004

Appl Environ Microbiol

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112

115

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Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of bacterial amplicons was predominated by those from members of the class Clostridia (57 of 93 clones), of which a phylotype most similar to that of the homoacetogen Acetobacterium sp. strain HAAP-1 was most abundant (32 of 93 clones). The remaining Bacteria consisted of phylotypes affiliated with Sphingobacteria, Bacteroides, Spirochaetes, Mollicutes, and Proteobacteria and candidate divisions OP11 and OP3. The two proteobacterial phylotypes were most similar to those of the known dechlorinators Trichlorobacter thiogenes and Sulfurospirillum multivorans. Although not represented by the bacterial clones generated with broad-specificity bacterial primers, a Dehalococcoides-like phylotype was identified with genus-specific primers. Only four distinct phylotypes were detected in the groundwater archaeal library, including predominantly a clone affiliated with the strictly acetoclastic methanogen Methanosaeta concilii (24 of 43 clones). A mixed culture that completely dechlorinates TCE to ethene was enriched from this groundwater, and both communities were characterized by terminal restriction fragment length polymorphism (T-RFLP). According to T-RFLP, the laboratory enrichment community was less diverse overall than the groundwater community, with 22 unique phylotypes as opposed to 43 and a higher percentage of Clostridia, including the Acetobacterium population. Bioreactor archaeal structure was very similar to that of the groundwater community, suggesting that methane is generated primarily via the acetoclastic pathway, using acetate generated by lactate fermentation and acetogenesis in both systems.Inadequate disposal practices of chlorinated solvents have resulted in the widespread contamination of groundwater with trichloroethene (TCE), a known toxin and suspected carcinogen. In situ biostimulation via anaerobic microbial reductive dechlorination is an attractive remediation strategy for TCE due to low operation and maintenance costs and minimal secondary-waste production relative to traditional extraction methods. Direct addition of nutrients such as electron donors to the contaminated subsurface can stimulate the activity of indigenous microbial communities. After oxygen is consumed, anaerobic populations reduce alternative electron acceptors such as nitrate, ferric iron, sulfate, and carbon dioxide (10). Chloroethenes can also be used as terminal electron acceptors (37,41,48,49), a process known as dehalorespiration, whereby hydrogen atoms sequentially replace chlorine atoms, leading to detoxification of the chloroethene...

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“…Though the extent of this plume is quite large, it does not appear to be migrating farther from the initial point of contamination. A previous study ruled out abiotic loss as the only reason for this observed attenuation (47). In addition, direct evidence that TCE come- Previously, methanotrophs, propanotrophs, nitrifiers, and phenol oxidizers have been isolated from groundwater within the TAN site (7).…”

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confidence: 99%

Coupling of Realistic Real Estimates with Genomics for Assessing Contaminant Attenuation and Long-Term Plume Containment

Crawford¹

2006

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2 NARRATIVE ACCOMPLISHMENTS OverviewThis report is a summary final report of the research undertaken at the University of Idaho (UI) over the past four years under DOE EMSP award DE-FG07-02ER63500. This work was performed collaboratively with investigators at the Idaho National Laboratory (INL) and Northwind Inc., who will provide separate summaries of their portions of the interdisciplinary project. The research centered on studies of the subsurface environment of the Snake River Plain Aquifer (SRPA) of the Test Area North (TAN) site at the INL in southeastern Idaho. Through this interdisciplinary effort, UI, INL, and Northwind, Inc. investigated coupled biogeochemical processes implicit in conceptual and mathematical models that describe trichloroethylene (TCE) co-metabolism in complex subsurface strata. Our team accomplished the following:• Investigated the microbial ecology of the SRPA related to known TCE cometabolizers.• Obtained evidence of biogenic methane in the SRPA that can sustain methanotrophic cometabolism of TCE.• Developed conceptual and mathematical models for natural attenuation at TAN and other DOE sites.• Used specific enzyme activities to detect TCE co-metabolic potential in SRPA microbes.• Acquired stable carbon isotope fractionation data for contaminants that demonstrate enhanced bioremediation of TCE.• Gained extensive field experience in subsurface microbiology, bioremediation, and design and operation of in situ bioreactors.• Two students received graduate degrees through their participation on the project.• One postdoctoral scientist received training through participation on the project.The following publications and presentations co-authored by members of the University of Idaho group were partially or completely made possible through the funding provided under award DE-FG07-02ER63500. Graduate Students TrainedTwo graduate students were trained during the project: J. L. Sebat, PhD and D. P. Erwin, MS. Postdoctoral Scientists TrainedOne postdoctoral scientist received experience during the project: Dr. Janice L. Strap SPECIFIC ACCOMPLISHMENTS Specific Accomplishment 1: find molecular markers of the methane monooxygenase (MMO) particulate form (pMMO) within the Test Area North (TAN) site of the Eastern Snake River Plain Aquifer (ESRPA) in IdahoIn this study we used primers specific for conserved regions of the pmoA gene of methane monooxygenase (MMO) particulate form (pMMO), the mmoX gene of MMO soluble form (sMMO), and the amoA gene of ammonia monooxygenase (AMO). We used the polymerase chain reaction (PCR) to amplify a variety of genes from whole community (metagenomic) DNA obtained directly from the aquifer. Based on these functional gene analyses, bacteria related to the type II methanotroph Methylocystis dominated both free-living and attached ESRPA communities. Type I sequences that grouped nearest to Methylobacter and Methylomonas were only obtained from the freeliving community. Sequences similar to the amoA gene associated with ammoniaoxidizing bacteria (AOB) most closely ma...

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An Evaluation of Aerobic Trichloroethene Attenuation Using First-Order Rate Estimation

Cited by 29 publications

References 25 publications

Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer

Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer

Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture

Coupling of Realistic Real Estimates with Genomics for Assessing Contaminant Attenuation and Long-Term Plume Containment

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