An evaluation of antibiotics for the elimination ofXanthomonas campestris pv.pelargonii (Brown) fromPelargonium x domesticum cv. ?Grand Slam? explants in vitro

Abstract

A range of antibiotics was evaluated for activity against Xanthomonas campestris pv. pelargonii (Xcp) on diagnostic sensitivity testing and plant tissue culture media. Many of the antibiotics showed reduced or no activity on the latter. Tetracycline and cefotaxime, chosen for further investigation, were screened for light stability under plant culture regimes. Tetracycline was inactivated in photosynthetic photon fluxes of 22 ~tmol m -2 s-1 and above. The minimum bacteriocidal concentration of cefotaxime was determined in bacteriological and plant tissue culture media. Cefotaxime was further tested for phytoxicity and ability to eliminate Xcp from deliberately infected explants. Cefotaxime was shown to eliminate contamination and stimulate the growth of the plant tissue cultures up 500 mg 1-1.

The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique - Review

“…In studies involving tropical peat swamp species such as G. bancanus (Chieng et al 2014a) and S. albida (Ishii, Mohsin 1994), sterilization techniques of submerging explants in 75% ethanol for 1 min followed by sterilization with 0.3 mol mercuric chloride for 5 min failed to eliminate fungus contaminations. It is interesting to note that, even though some experiments may give comparatively highly clean culture when tested using a particular technique, these results are not usually replicable on other tissue culture work and so the expected results are usually elusive (Barrett, Cassells 1994). In many cases anti-microbial treatments only inhibit contaminants and low levels of contamination persist even though some researchers incorporated the use of antibiotics and fungicides to overcome the contamination problem (Leifert, Cassells 2001).…”

A risk assessment study of plant genetic transformation usingAgrobacterium and implications for analysis of transgenic plants

“…If there was less growth than the control, then there was some bactericidal effect and the minimum concentration that yields no growth is the MBC value. Incorporation of antibiotic into the tissue culture medium, at a concentration of four times the MBC is recommended for elimination of bacteria in tissue culture (Falkiner pers, comm; Barrett and Cassells, 1994).…”

“…This technique, however, is unreliable since bacteria can remain latent within plant tissue throughout the culture period (Cassells, 1991;Leifert et al, 1991). Antibiotics have been successful in the elimination of bacteria in tissue culture in some cases (Horsch and King, 1983;Leifert et al, 1991;Barrett and Cassells, 1994), but a vital aspect to their success is to ensure the completion of drug sensitivity tests, MBC determination, stability of the antibiotic and phytotoxicity to the plant.…”

The effects of antibiotics and antifungals added to plant culture media of Triticosecale wittmarck

“…3 Cefotaxime was shown to eliminate contamination with Xanthomonas campestris pv.pelargonii and stimulate the growth of the Pelargonium x domesticum cv. 'Grand Slam' plant tissue cultures (Barrett and Cassells, 1994). Table 2.…”