An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

Antonioli, Eliane; Lobo, Anderson Oliveira; Ferretti, Mário; Cohen, Moisés; Marciano, Fernanda Roberta; Corat, Evaldo José; Trava-Airoldi, V.J.

doi:10.1016/j.msec.2012.10.010

Cited by 23 publications

(24 citation statements)

References 45 publications

(59 reference statements)

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“…A hydrophilic polyurethane matrix promoted chondrogenesis of MSCs [41]. Superhydrophilic vertically aligned carbon nanotubes have permitted the adhesion and maintenance of human chondrocytes [42]. However, the results from our present study indicated that the proliferation of the hMSCs was not significantly different during the 48-h cultivation on various Ti-based coatings on silicon.…”

Section: Discussioncontrasting

confidence: 58%

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

Kaitainen

Kröger

et al. 2016

Materials

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The chemical composition and texture of titanium coatings can influence the growth characteristics of the adhered cells. An enhanced proliferation of the human mesenchymal stem cells (hMSCs) would be beneficial. The present study was aimed to investigate whether titanium deposited at different atmospheres would affect the cell growth properties, cellular morphology, and expression of surface markers of hMSCs. Titanium-based coatings were deposited on silicon wafers under oxygen, nitrogen, or argon atmospheres by ultra-short pulsed laser deposition using two different gas pressures followed by heating at 400 °C for 2 h. The characteristics of the coated surfaces were determined via contact angle, zeta potential, and scanning electron microscopy (SEM) techniques. Human MSCs were cultivated on differently coated silicon wafers for 48 h. Subsequently, the cell proliferation rates were analyzed with an MTT assay. The phenotype of hMSCs was checked via immunocytochemical stainings of MSC-associated markers CD73, CD90, and CD105, and the adhesion, spreading, and morphology of hMSCs on coated materials via SEM. The cell proliferation rates of the hMSCs were similar on all coated silicon wafers. The hMSCs retained the MSC phenotype by expressing MSC-associated markers and fibroblast-like morphology with cellular projections. Furthermore, no significant differences could be found in the size of the cells when cultured on all various coated surfaces. In conclusion, despite certain differences in the contact angles and the zeta potentials of various titanium-based coatings, no single coating markedly improved the growth characteristics of hMSCs.

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Section: Discussioncontrasting

confidence: 58%

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

Kaitainen

Kröger

et al. 2016

Materials

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“…This result can be attributed to the favorable cell adhesion on bare CNT. Hydrophilic CNT forest can also be used for cell cultures [82]. Superhydrophilic forest of CNTs was obtained by exposure of CNT forest to oxygen plasma.…”

Section: Three-dimensional Nanoarchitectures From Carbon Nanotubes Fomentioning

confidence: 99%

Bioactive nanocarbon assemblies: Nanoarchitectonics and applications

et al. 2014

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“…Several papers have been published by our group reporting the use of superhydrophilic MWCNTO-GO as is or in bioscaffolds in contact with different cell lines [39][40][41][42]. In all of these studies, we have demonstrated their non-cytotoxicity and tissue regeneration ability using animal models [43].…”

Section: Biological Assaysmentioning

confidence: 82%

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

Silva

Vasconcellos

Rodrigues

et al. 2017

Materials Science and Engineering: C

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Herein, we developed honeycomb-like scaffolds by combining poly (d, l-lactic acid) (PDLLA) with a high amount of graphene/multi-walled carbon nanotube oxides (MWCNTO-GO, 50% w/w). From pristine multi-walled carbon nanotubes (MWCNT) powders, we produced MWCNTO-GO via oxygen plasma etching (OPE), which promoted their exfoliation and oxidation. Initially, we evaluated PDLLA and PDLLA/MWCNTO-GO scaffolds for tensile strength tests, cell adhesion and cell viability (with osteoblast-like MG-63 cells), alkaline phosphatase (ALP, a marker of osteoblast differentiation) activity and mineralized nodule formation. In vivo tests were carried out using PDLLA and PDLLA/MWCNTO-GO scaffolds as fillers for critical defects in the tibia of rats. MWCNTO-GO loading was responsible for decreasing the tensile strength and elongation-at-break of PDLLA scaffolds, although the high mechanical performance observed (~600MPa) assures their application in bone tissue regeneration. In vitro results showed that the scaffolds were not cytotoxic and allowed for osteoblast-like cell interactions and the formation of mineralized matrix nodules. Furthermore, MG-63 cells grown on PDLLA/MWCNTO-GO significantly enhanced osteoblast ALP activity compared to controls (cells alone), while the PDLLA group showed similar ALP activity when compared to controls and PDLLA/MWCNTO-GO. Most impressively, in vivo tests suggested that compared to PDLLA scaffolds, PDLLA/MWCNTO-GO had a superior influence on bone cell activity, promoting greater new bone formation. In summary, the results of this study highlighted that this novel scaffold (MWCNTO-GO, 50% w/w) is a promising alternative for bone tissue regeneration and, thus, should be further studied.

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An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

Cited by 23 publications

References 45 publications

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings

Bioactive nanocarbon assemblies: Nanoarchitectonics and applications

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

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