An evaluation of immunomagnetic separation for the detection of salmonellas in raw chicken carcasses

Coleman, David; Chick, K.E.; Nye, K.

doi:10.1111/j.1472-765x.1995.tb01029.x

Cited by 21 publications

(9 citation statements)

References 3 publications

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“…The technique, which employs magnetic beads, has been used since the 1970's when Norwegian scientists tried to apply magnetism for biological investigation. IMS has been used successfully by some authors to replace enrichment in selective media (Coleman, Chick, & Nye, 1995). Thus far, the IMS/qRT-PCR method has been used to concentrate the virus and minimize inhibitors from food.…”

Section: Discussionmentioning

confidence: 99%

Recovery of structurally intact norovirus from food-contact surfaces

Kim²,

Choi

et al. 2015

Food Control

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Section: Discussionmentioning

confidence: 99%

Recovery of structurally intact norovirus from food-contact surfaces

Kim²,

Choi

et al. 2015

Food Control

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“…Previous work has shown that immunomagnetic separation (IMS) techniques may offer an advantage in improving microbiological systems for the detection of salmonellae (Coleman et al 1995). However, problems of specificity still remained, with a variety of organisms, notably mucoid coliforms, Citrobacter freundzz, pseudomonads and Proteus spp.…”

Section: Introductionmentioning

confidence: 99%

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

Coleman

Nye

Chick

et al. 1995

Lett Appl Microbiol

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Immunomagnetic separation with additional enrichment was used in conjunction with improved selective media to improve the isolation of salmonellae from raw sausages. The isolation rate achieved was almost double that of conventional culture with no increase in processing time. The selective media gave an overall specificity of approximately 74%; all false-positive pickoffs being identified as Citrobacter freundii. It is believed that this method represents a significant advance in the isolation of salmonellae from foods, although the ideal media both for enrichment and selection have yet to be found.

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“…1998). Improved isolation rates have been described when using IMS in the isolation of salmonellae from raw chicken carcasses (Coleman et al. 1995a), skimmed milk powder (Dziadkowiec et al.…”

Section: Discussionmentioning

confidence: 99%

Application of an automated immunomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises

Duncanson

Wareing

Jones³

2003

Lett Appl Microbiol

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Aims:The application of an automated immunomagnetic separation-enzyme immunoassay (AIMS-EIA) during the investigation of a suspected outbreak of Salmonella food poisoning at a retail premises. Methods and Results: Six food samples and 24 environmental swabs were taken from the retail premises and six food handlers' submitted faecal samples during the investigation of the outbreak. Isolation and identification of Salmonella from these samples was performed according to established standard operating procedures and by AIMS-EIA. Twelve of the 18 (67%) Salmonella culture positive samples were AIMS-EIA positive on testing preenrichment samples after 24 h, whilst 17 (94%) samples were AIMS-EIA positive following selective enrichment for a further 48 h. One food handler was found to be positive for Salmonella by both culture and AIMS-EIA. All Salmonella isolates were confirmed as Salmonella Enteritidis phagetype 21c. Conclusions: The AIMS-EIA protocol compliments the conventional culture approach to produce more timely results for the management of the risk to public health without significantly increasing the workload of the laboratory. Significance and Impact of the Study: The food production premise investigated in this study was heavily contaminated with Salmonella Enteritidis. Application of the AIMS-EIA was significant in the effective intervention of control measures for the protection of public health.

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An evaluation of immunomagnetic separation for the detection of salmonellas in raw chicken carcasses

Cited by 21 publications

References 3 publications

Recovery of structurally intact norovirus from food-contact surfaces

Recovery of structurally intact norovirus from food-contact surfaces

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

Application of an automated immunomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises

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