An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm

Yıldız, Cengiz; Bozkurt, Yusuf; Yavaş, İlker

doi:10.1016/j.cryobiol.2013.05.008

Cited by 33 publications

(30 citation statements)

References 33 publications

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“…) and fish (Yildiz et al . ). The difference between our results and previous studies may be due to the difference in soybean lecithin sources, the preparation for soybean lecithin extenders and the concentrations of soybean lecithin.…”

Section: Discussionmentioning

confidence: 97%

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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Section: Discussionmentioning

confidence: 97%

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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“…We chose to use soya bean lecithin P5638 from Sigma because it has previously been used to cryopreserve semen from fish (Yildiz et al. ) and stallion (Papa et al. ) with post‐thaw sperm motility and membrane integrity similar to that of spermatozoa preserved in an egg yolk extender.…”

Section: Discussionmentioning

confidence: 99%

“…The exact type of lecithin is not always mentioned in published studies. We chose to use soya bean lecithin P5638 from Sigma because it has previously been used to cryopreserve semen from fish (Yildiz et al 2013) and stallion (Papa et al 2011) with post-thaw sperm motility and membrane integrity similar to that of spermatozoa preserved in an egg yolk extender. Our results were, however, inferior to the results by Beccaglia et al (2009b) using a similar composition of the Tris extender for cryopreservation of dog semen but a different lecithin concentration and a different soya bean lecithin source.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk

Axnér

Lagerson

2016

Reprod Domestic Animals

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Egg yolk is usually included in extenders used for preservation of dog semen. Lecithin is an interesting animal-protein free alternative to egg yolk for semen preservation. The aim of our study was to evaluate soya bean lecithin for cryopreservation of dog semen. Five ejaculate replicates were divided in three equal parts, centrifuged and each pellet diluted with one of the three Tris-based extenders containing 20% egg yolk, 1% soya bean lecithin or 2% soya bean lecithin. Extended semen was loaded in 0.5-ml straws, cooled and diluted a second time and frozen in liquid nitrogen vapours. Sperm motility parameters (CASA), acrosome integrity (FITC-PNA/PI) and sperm membrane integrity (C-FDA) were evaluated 5 min post-thaw and after 2 and 4 h of incubation. Total motility was significantly better in the egg yolk extender than in any of the lecithin-based extender and was better in the 1% lecithin extender than in the 2% lecithin extender. Sperm membrane integrity was significantly better in the egg yolk extender than in any of the lecithin-based extenders but did not differ significantly between the 1% and 2% lecithin extenders. Acrosome integrity was significantly better in the egg yolk extender than in the 2% lecithin extender but did not differ between the egg yolk extender and the 1% lecithin extender or between the two lecithin extenders. In conclusion, egg yolk was superior to lecithin in our study. The extender with 1% lecithin preserved sperm motility better than the extender with 2% lecithin.

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“…The advantages of soya lecithinbased semen extenders (SL) over egg yolk regarding sanitary issues are unquestionable but the efficacy of SL is still a matter of debate. In recent years, commercially available SL have been tried in many species and claimed equivalent (Forouzanfar et al, 2010;Yildiz et al, 2013), superior (Viviana et al, 2003) and inferior (Celeghini et al, 2008;Crespilho et al, 2012) to EY.…”

Section: Introductionmentioning

confidence: 98%

Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen

Kumar

Saini

Kumar

et al. 2015

Animal Reproduction Science

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An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm

Cited by 33 publications

References 33 publications

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk

Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen

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