Moisture damage is the most critical issue regarding the preservation and integrity of cultural heritage sites. The electromagnetic (EM) sensitivity to the presence of moisture, in both soils and structural materials, is a well-known phenomenon. Thereby, studying the EM response to the presence of moisture, in order to prevent the damages done to sites of cultural heritage, is a well-established method. This paper will discuss the ability of a geophysical non-destructive technique (NDT), present in a Ground Penetrating Radar (GPR) system, to investigate a very precious building in Rome that is affected by a moisture problem (the Turkish Room at Villa Medici). This geophysical instrument is able to locate and estimate the extent of water ingression, which can aid in the development of restoration plans before permanent damage occurs. The main objective of this paper is to help restorers understand the related hazards, due to the presence of moisture in the wall structures, in real-time and to rapidly and non-invasively develop strategies for the preservation of cultural heritage sites.