Abstract. During the TROCCINOX field experiment in January and February 2005, the contribution of lightninginduced nitrogen oxides (LNOx) from tropical and subtropical thunderstorms in Southern Brazil was investigated. Airborne trace gas measurements (NO, NO y , CO and O 3 ) were performed up to 12.5 km with the German research aircraft Falcon. During anvil penetrations in selected tropical and subtropical thunderstorms of 4 and 18 February, NO x mixing ratios were on average enhanced by 0.7-1.2 and 0.2-0.8 nmol mol −1 totally, respectively. The relative contributions of boundary layer NO x (BL-NOx) and LNOx to anvil-NO x were derived from the NO x -CO correlations. On average ∼80-90% of the anvil-NO x was attributed to LNOx. A Lightning Location Network (LINET) was set up to monitor the local distribution of cloud-to-ground (CG) and intracloud (IC) radiation sources (here called "strokes") and compared with lightning data from the operational Brazilian network RINDAT (Rede Integrada Nacional de Detecção de Descargas Atmosféricas). The horizontal LNOx mass flux out of the anvil was determined from the mean LNOx mixCorrespondence to: H. Huntrieser (heidi.huntrieser@dlr.de) ing ratio, the horizontal outflow velocity and the size of the vertical cross-section of the anvil, and related to the number of strokes contributing to LNOx. The values of these parameters were derived from the airborne measurements, from lightning and radar observations, and from a trajectory analysis. The amount of LNOx produced per LINET stroke depending on measured peak current was determined. The results were scaled up with the Lightning Imaging Sensor (LIS) flash rate (44 flashes s −1 ) to obtain an estimate of the global LNOx production rate. The final results gave ∼1 and ∼2-3 kg(N) per LIS flash based on measurements in three tropical and one subtropical Brazilian thunderstorms, respectively, suggesting that tropical flashes may be less productive than subtropical ones. The equivalent mean annual global LNOx nitrogen mass production rate was estimated to be 1.6 and 3