An evaluation of total 25-hydroxyvitamin D assay standardization: Where are we today?

Bjerg, Lise Nørkjær; Halgreen, Jens Romlund; Hansen, Steen Holger; Morris, Howard A.; Jørgensen, Niklas Rye

doi:10.1016/j.jsbmb.2019.03.015

Cited by 28 publications

(18 citation statements)

References 23 publications

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“…The analytical difficulties related to the measurement of vitamin D metabolites may lead to misclassification of ‘low' vitamin D status. The variations in analytical techniques and assays may cause the large variability in laboratory measurements of vitamin D status and the systematic “errors” [ 36 – 45 ]. In our study, the serum 25(OH)D level of ≥30 ng/ml was considered as the normal level and 20 to 29 ng/ml as vitamin D insufficiency, whereas serum 25(OH)D levels of <20 ng/ml were considered as an indicative of vitamin D deficiency.…”

Section: Discussionmentioning

confidence: 99%

“Vitamin D Deficiency Is More Common in Women with Autoimmune Thyroiditis: A Retrospective Study”

Turashvili

Javashvili

Giorgadze

2021

International Journal of Endocrinology

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Background. Vitamin D is a hormone that is mainly produced in the skin upon ultraviolet B radiation exposure and has important influence on various organs. In recent years, data have been collected that vitamin D deficiency plays an important role in the development of various nonskeletal diseases, including autoimmune diseases. Chronic autoimmune thyroiditis (Hashimoto’s thyroiditis) is one of the most common organ-specific autoimmune endocrine diseases. It is characterized by increased level of antithyroid peroxidase and/or antithyroglobulin antibodies in blood, which often leads to thyroid dysfunction and structural changes of the gland. There is an opinion that vitamin D deficiency may be considered as an important risk factor for development of chronic autoimmune thyroiditis, but data of various small studies are controversial. Despite the fact that Georgia is a sunny country, vitamin D deficiency is a widespread problem here. Thyroid diseases, including the chronic autoimmune thyroiditis, are also very common in Georgia. The aim of our research was to compare the level of vitamin D between the patients with chronic autoimmune thyroiditis and the healthy subjects. Methods. This retrospective study enrolled subjects, who were 18–70 years old and visited the clinics “Cortex” and “National Institute of Endocrinology” in 2018 or in 2019 from mid-spring to mid-summer. Data of thyroid-stimulating hormone, free thyroxine, antithyroid peroxidase antibodies, antithyroglobulin antibodies, thyroid ultrasonography, and 25(OH) vitamin D were retrospectively analysed based on medical history. In total, data of 1295 patients were collected. The statistical processing of data was performed through the SPSS 20 program. Results. The negative association between thyroid-stimulating hormone, antithyroid peroxidase antibodies, antithyroglobulin antibodies, heterogeneous parenchyma of thyroid gland, and vitamin D was found in women. Statistically significant association was not detected in men. Conclusions. Serum vitamin D is lower in women with autoimmune thyroiditis and primary hypothyroidism. Further studies are needed to evaluate the influence of vitamin D supplementation on thyroid autoantibody positivity or primary hypothyroidism.

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Section: Discussionmentioning

confidence: 99%

“Vitamin D Deficiency Is More Common in Women with Autoimmune Thyroiditis: A Retrospective Study”

Turashvili

Javashvili

Giorgadze

2021

International Journal of Endocrinology

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“…Due to this analytical variability and pre‐analytical factors for serum 25‐OHD variation (season, sun exposure, and skin type), no evidence‐based international consensus on a human vitamin D RI exists 26 . It is, however, recognized that 25‐OHD concentrations below 30 nmol/L increase the risk of poor musculoskeletal health, while concentrations between 50‐125 nmol/L appear to be safe and sufficient 27‐29 . Cases of vitamin D toxicity are rare and associated with serum 25‐OHD concentrations above 300 nmol/L 28…”

Section: Discussionmentioning

confidence: 99%

“…26 It is, however, recognized that 25-OHD concentrations below 30 nmol/L increase the risk of poor musculoskeletal health, while concentrations between 50-125 nmol/L appear to be safe and sufficient. [27][28][29] The CV of 6% calculated between the DBS1 and DBS2 pairs encompass both the within-batch analytical CV of the assay for chimpanzees (intra-assay variation) and the variations due to the DBS quality (spot size, diffusion of blood). The result is comparable to the reported intra-assay CV for the human DBS assay of <10%, thus suggesting little variation due to DBS quality in this study.…”

Section: Discussionmentioning

confidence: 99%

Comparison of 25‐hydroxyvitamin D concentration in chimpanzee dried blood spots and serum

Moittié

Graham

Barlow

et al. 2020

Veterinary Clinical Pathol

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“…An essential point that needs repeating is that laboratories cannot assume that just because an immunoassay assay is CDC-certified it will function appropriately in their hands (89,90) . We recommend a testing period in order to verify that an immunoassay is standardised especially since there is generally very little an individual laboratory can do to 'calibrate' an immunoassay.…”

Section: Vdsp: a Ready Solutionmentioning

confidence: 99%

25-Hydroxyvitamin D assay standardisation and vitamin D guidelines paralysis

Sempos¹,

Binkley

2020

Public Health Nutr.

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An evaluation of total 25-hydroxyvitamin D assay standardization: Where are we today?

Cited by 28 publications

References 23 publications

“Vitamin D Deficiency Is More Common in Women with Autoimmune Thyroiditis: A Retrospective Study”

“Vitamin D Deficiency Is More Common in Women with Autoimmune Thyroiditis: A Retrospective Study”

Comparison of 25‐hydroxyvitamin D concentration in chimpanzee dried blood spots and serum

25-Hydroxyvitamin D assay standardisation and vitamin D guidelines paralysis

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