An Examination of the Crabtree Effect in Saccharomyces cerevisiae: the Role of Respiratory Adaptation

Barford, J. P.; Hall, Robert J.

doi:10.1099/00221287-114-2-267

Cited by 119 publications

(65 citation statements)

References 32 publications

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“…Under all conditions investigated, once the maximum respiration rate was reached, it was maintained when the substrate supply rate was increased further. A similar observation was reported by Barford & Hall (1979b). They found an identical maximum respiration rate on glucose and on galactose and also were not able to detect any repressive effect of high glucose consumption rates on the respiratory activity of their strain of baker's yeast.…”

Section: Discussionsupporting

confidence: 72%

“…The fact that fully derepressed growth of S. cerevisiae on glucose can also be observed in a chemostat culture indicates that the primary cause of repression is not the presence of the substrate per se, but rather the metabolic flux generated by the uptake of substrate into the cell (Schatzmann, 1975;Bijkerk & Hall, 1977;Barford & Hall, 1979b). This would indicate that there must be a particular substrate uptake rate for a certain strain of S. cereoisiue which would be characteristic of the onset of the glucose effect in this organism.…”

Section: Introductionmentioning

confidence: 99%

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The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

Rieger¹,

Käppeli²,

Fiechter³

1983

Microbiology

121

145

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\The respiratory capacity of Saccharomyces cerevisiae growing in continuous culture on glucose and on mixtures of glucose and ethanol was investigated. An oxygen uptake rate of 8 mmol g-l h-l was found to limit the ability of the organism to degrade a substrate purely oxidatively. On glucose as sole energy and carbon source, this respiration rate was invariably achieved at an identical growth rate and thus at an identical substrate uptake rate when the inlet glucose concentration was varied. The rate of ethanol co-consumption together with glucose was strictly governed by this limiting maximum respiratory capacity and no repression of respiration was observed at dilution rates where ethanol was excreted by the cells. Hence, a limitation in some step in the oxidative branch of catabolism is likely to be responsible for incomplete oxidation of glucose at high growth rates rather than an undefined action of glucose repression.

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

Rieger¹,

Käppeli²,

Fiechter³

1983

Microbiology

121

145

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“…The method developed by von Meyenburg (1969) involved the construction of gas balances for S. cerevisiae at a number of different dilution rates, both in the fully respiratory range of (Barford & Hall 1979;Rieger et al 1983). Furthermore, it is highly unlikely that YA'r• is constant at all growth rates, since it is well known that the protein content of S. cerevisiae increases significantly with increasing growth rate (Furukawa et al 1983;Verduyn et al 1990a;Fiechter & v o n Meyenburg 1969), both under anaerobic and aerobic conditions.…”

Section: Calculation Of P/o-ratios For Growth On Glucosementioning

confidence: 99%

A theoretical evaluation of growth yields of yeasts

Verduyn

Stouthamer

Scheffers

et al. 1991

Antonie van Leeuwenhoek

216

176

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Growth yields of Saccharomyces cerevisiae and Candida utilis in carbon-limited chemostat cultures were evaluated. The yields on ethanol and acetate were much lower in S. cerevisiae, in line with earlier reports that site I phosphorylation is absent in this yeast. However, during aerobic growth on glucose both organisms had the same cell yield. This can be attributed to two factors: -S. cerevisiae had a lower protein content than C. utilis; -uptake of glucose by C. utilis requires energy whereas in S. cerevisiae it occurs via facilitated diffusion. Theoretical calculations showed that, as a result of these two factors, the ATP requirement for biomass formation in C. utilis is 35% higher than in S. cerevisiae (theoretical YATP values of 20.8 and 28.1, respectively). The experimental YATP for anaerobic growth of S. cerevisiae on glucose was 16 g biomass-mol ATP -1.In vivo P/O-ratios can be calculated for aerobic growth on ethanol and acetate, provided that the gap between the theoretical and experimental ATP requirements as observed for growth on glucose is taken into account. This was done in two ways: -via the assumption that the gap is independent of the growth substrate (i.e. a fixed amount of ATP bridges the difference between the theoretical and experimental values). -alternatively, on the assumption that the difference is a fraction of the total ATP expenditure, that is dependent on the substrate. Calculations of P/O-ratios for growth of both yeasts on glucose, ethanol, and acetate made clear that only by assuming a fixed difference between theoretical and experimental ATP requirements, the P/O-ratios are more or less independent of the growth substrate. These P/O-ratios are approximately 30% lower than the calculated mechanistic values.

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“…The yeast was grown under carbon limited fully aerobic chemostat culture conditions as described previously (17,18). Steady state was defined as occurring when the major culture variables (dry weight, specific carbon dioxide production rate, specific oxygen uptake rate, sugar concentration, and ethanol concentrations) did not vary by more than 5 % over three consecutive samplings.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the variation of cell length during the cell cycle of Schizosaccharomyces pombe using an asynchronous chemostat culture.

Barford

1985

J. Gen. Appl. Microbiol.

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A method enabling the analysis of the variation of cell length during the cell cycle of Schizosaccharomyces pombe using an asynchronous chemostat culture was developed. It was used to experimentally test the proposal that for the first 75 % of the cell cycle the cell grows only in length and during the last 25 % of the cell cycle there is no change in cell length. The results obtained question the validity of this proposal and are consistent with a control in which, as specific growth rate (or equivalently energy source availability) increases, a smaller portion of the cell cycle is required for the total length increase which occurs during the cell cycle. This is consistent with "critical mass" theories for the initiation of various cellular events which would be expected to be initiated at an earlier stage of the cell cycle in the case of rapid growth.

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An Examination of the Crabtree Effect in Saccharomyces cerevisiae: the Role of Respiratory Adaptation

Cited by 119 publications

References 32 publications

The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

A theoretical evaluation of growth yields of yeasts

Analysis of the variation of cell length during the cell cycle of Schizosaccharomyces pombe using an asynchronous chemostat culture.

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