An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention

Abstract

A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieriatrifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails of some highly viscous material on the walls of the suspension vessels or developed a thick overlay on semisolid media without adversities in plant growth. FTIR identified this substance to be an extracellular polysaccharide. Various morphological, biochemical tests, and molecular analyses using 16S rRNA, atpD, and recA genes characterized this isolate JAS1 as a novel strain of Agrobacteriumpusense. Its mucoidal growth over Murashige and Skoog media yielded enormous exopolysaccharide (7252 mg l−1), while in nutrient agar it only developed fast-growing swarms. As a qualifying plant growth-promoting bacteria, it produces significant indole-3-acetic acid (86.95 mg l−1), gibberellic acid (172.98 mg l−1), ammonia (42.66 µmol ml−1). Besides, it produces siderophores, 1-aminocyclopropane-1-carboxylicaciddeaminase, fixes nitrogen, forms biofilms, and productively solubilizes soil inorganic phosphates, and zinc. Under various treatments with JAS1, wheat and chickpea resulted in significantly enhanced shoot and root growth parameters. PGP effects of JAS1 positively enhanced plants’ physiological growth parameters reflecting significant increments in overall chlorophyll, carotenoids, proline, phenols, flavonoids, and sugar contents. In addition, the isolated strain maintained both plant and soil health under an intermittent soil drying regime, probably by both its PGP and EPS production attributes, respectively.

Green synthesis of silver nano particles using Dracaena Trifasciata plant extract and comparison of fatty acids and amides capping agents

“…For the Dracaena Trifasciata plant extract as shown in figure 6(a), IR bands have the peak at 3298 and 1741 cm −1 are the characteristics of the stretching modes C=O and O-H which are attributed to the amino groups exists in phenol and alcohol and amines that can also be find in the aloe vera plant extract which is used for the green synthesis of the NPs [43]. These groups are possibly due to the presence of luteolin-7-glucoside and apigenin-7-glucoside in the Dracaena Trifasciata plant extract [44]. Due to the carbonyl stretching, amide I raised that corresponding to 1631 cm −1 .…”

Enhancing drought tolerance in cauliflower (Brassica oleracea var. botrytis) by targeting LFY transcription factor modulation via the ethylene precursor, ACCA: an innovative computational approach

“…Our in-depth computational examination of the LFY-ACCA complex revealed important insights. We focused on understanding the roles of various molecular interactions, such as lipophilic, van der Waals, and hydrogen bonds, in maintaining the stability of this complex ( Kaur et al., 2022 ). Moreover, molecular dynamics simulations provided predictions on the temporal behavior of the LFY-ACCA complex, consistently indicating its stability, which is pivotal for the prolonged and effective modulation of LFY function.…”

“…It is most obvious on the sides of the inflorescence meristem in floral anlagen and in floral primordia at the earliest stages of development ( Weigel et al., 1992 ). Therefore, our aim in this research is to protect the cauliflower plant from these attacks; a new strain of Agrobacterium pusense , JAS1, possesses some compounds that act as plant-growth-promoting bacteria ( Kaur et al., 2022 ). It also makes ammonia, 1-aminocyclopropane-1-carboxylic acid, indole-3-acetic acid, gibberellic acid, and siderophores, which help with fixing nitrogen, making biofilms.…”

Exploring the efficacy of 1-amino-cyclopropane-1-carboxylic acid (ACCA) as a natural compound in strengthening maize resistance against biotic and abiotic stressors: an empirical computational study

“…To combat these challenges, researchers have explored the potential of utilizing beneficial compounds derived from Agrobacterium pusense strain JAS1. This strain is known for its nitrogen fixation and biofilm formation capabilities ( Kaur et al, 2022 ). The strain produces various chemicals contributing to plant immune-boosting, root hair growth, and shoot development.…”

“…ACCA was the most effective compound against maize, as it induced a virulence system that enhanced the plant’s defense against pathogenic attacks. By modulating ethylene biosynthesis, ACCA can alter plant defense mechanisms, allowing plants to cope better with various stress factors ( Jorgensen et al, 1996 ; Bowers et al, 2006 ; Chow et al, 2008 ; Shaw et al, 2010 ; Shivakumar et al, 2010 ; Meng et al, 2011 ; Ferreira et al, 2015 ; Kaur et al, 2022 ; Debnath et al, 2022a , b ; Mukerjee et al, 2022b , c ; APEDA, 2023 ; Debnath et al, 2023 ).…”