2010
DOI: 10.1002/elps.201000196
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An expanding negative detection method for the visualization of DNA in polyacrylamide gels by eosin Y

Abstract: A sensitive and easy technique has been developed for the negative detection of DNA following PAGE using eosin Y. After electrophoresis, gels are fixed and stained within 40 min to provide a detection limit of 0.1-0.2 ng of single DNA band, which appears as transparent and colorless under the opaque gel matrix background. The sensitivity of the new stain is fourfold better than zinc-imidazole negative and ethidium bromide stains. Furthermore, the newly developed staining method has been successfully applied to… Show more

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Cited by 8 publications
(3 citation statements)
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“…The supernatants were removed, and the oily precipitates were washed with Tris-HCl (pH 7.4, 5.0 mm, 1.0 mL). To determine the number of TBA molecules per AuNP, the amount of TBA in the supernatant after centrifugation was investigated by electrophoresis on a 12 % polyacrylamide gel compared with a DNA marker (20 base pair DNA ladder); [22] UV trans illumination (Bio-vision E-Box, Vilber Loumat, France) of the gel was employed using HealthView nucleic acid stain (Genomics BioSci&Tech, Taipei, Taiwan) to visualize the DNA. In this study, we utilized ImageJ software (NIH, USA) to quantify the intensity of bands.…”
Section: Methodsmentioning
confidence: 99%
“…The supernatants were removed, and the oily precipitates were washed with Tris-HCl (pH 7.4, 5.0 mm, 1.0 mL). To determine the number of TBA molecules per AuNP, the amount of TBA in the supernatant after centrifugation was investigated by electrophoresis on a 12 % polyacrylamide gel compared with a DNA marker (20 base pair DNA ladder); [22] UV trans illumination (Bio-vision E-Box, Vilber Loumat, France) of the gel was employed using HealthView nucleic acid stain (Genomics BioSci&Tech, Taipei, Taiwan) to visualize the DNA. In this study, we utilized ImageJ software (NIH, USA) to quantify the intensity of bands.…”
Section: Methodsmentioning
confidence: 99%
“…Eosin Y (EY, 2 0 ,4 0 ,5 0 ,7 0 -tetrabromofluorescein disodium salt), a heterocyclic dye containing bromine atoms, since its introduction in 1986 for detecting electrophoretically resolved proteins, has rapidly been improved into a large scale detection method for protein and DNA in polyacrylamide gel due to its high sensitivity and ease of use. [19][20][21] As another embodiment particularly preferred for staining electrophoresis gels, in this study, EY has been extended to DNA detection in agarose gel with an environmentally benign and sensitive protocol. The newly developed method provides a detection limit down to 0.2-0.6 ng DNA band, only by 10 min fixing followed with 30 min staining and 15-20 min developing.…”
Section: Introductionmentioning
confidence: 99%
“…Its structure is shown in Figure S1 of the Supporting Information. Because of its stability and complex aromatic structure, eosin Y is used in the fields of leather, printing, dyeing, printing ink, fluorescent pigment, photocatalyst, and so on. Since it was used to act as a reversible stainer for detecting electrophoretically resolved proteins in 1986, eosin Y has rapidly been improved into a large-scale detection method for protein and DNA in polyacrylamide gel due to its high sensitivity and ease of use. As a model for organic compounds, eosin Y has been rarely extended to interact with the cationic polymer. Up to now, there is no report on the interaction of Eosin Y, as a fluorescent probe, with PEI to form the eosin Y/PEI complex.…”
mentioning
confidence: 99%