An experimental investigation into the development of callus and induced bone tumours in mice studied by histological and enzyme histochemical methods

Abstract

THE development of a tumour can be taken as an expression of a disturbed equilibrium between the number of undifferentiated and differentiated cells of a tissue, in favour of the number of undifferentiated cells. Irrespective of the cause of this disorder, the derailed cells can show morphological, biochemical and immunological deviations. The purpose of this investigation was to compare the histochemical and enzyme histochemical pattern of cells of regenerating bone tissue and of cells of induced bone tumours. To determine the enzyme pattern of cells in regenerating bone tissue healing fractures have been studied. Several methods of inducing bone tumours are known: Radioactive compounds such as 45Ca, 89Sr, 90Sr, 239Pu and 226Ra (Anderson,

Histological and Histochemical Findings of Transplantable Osteosarcoma of Mice

“…Walker (45) suggested that osteoblasts synthesized L-proline and L-hydroxyproline of collagen from pyruvate and osteoclasts participated in the reversed pathway on the basis of histochemical findings of several dehydrogenase, such as succinic dehydrogenase in osteoclasts and osteoblasts. Histochemical behavior of the giant cells of this osteosarcoma was similar to that of osteoclasts reported by many investigators on the activity of alkaline phosphatase (31,35,44), acid phosphatase (31,35,44) and succinic dehydrogenase (5,45). Based on the aforementioned observations and the site and time of appearance of the giant cells in the graft, it seems possible that the giant cells functionally might have no relationship to osteolysis, bone absorption and intracellular digestion.…”

“…De Duve et al (S) described that this enzyme existed in lysosome and had a role in intracellular digestion by tissue fractionation studies. Histochemically, acid phosphatase activity was observed in foreign body giant cells (4,31), macrophages (6), osteoclasts (4,6,20,44), giant cells in osteosarcoma and giant cell tumors of bone (4,14,20,31,35,44). Pepler (31) and Schajowicz (35) described a close resemblance between osteoclasts and giant cells of osteosarcoma and of giant cell tumors of the bone.…”

“…In this experiment, the giant cells showed succinic dehydrogenase activity as well as acid phosphatase activity. Positive activity of succinic dehydrogenase was demonstrated in osteoclasts by Burstone (5), and in giant cells of osteosarcoma or giant cell tumor of bone by Schajowicz (35) and Timmer et al (44). Walker (45) suggested that osteoblasts synthesized L-proline and L-hydroxyproline of collagen from pyruvate and osteoclasts participated in the reversed pathway on the basis of histochemical findings of several dehydrogenase, such as succinic dehydrogenase in osteoclasts and osteoblasts.…”

Immunohistochemical and biochemical study of alkaline phosphatase in bone tumors and tumorous conditions - With special notes on giant cells.

“…ALPase is known to have various isoenzymes, and the bone type isoenzyme, in particular, is considered to be related closely to bone metabolism (7,11,23,29). However, its localization and role in bone tumor tissues are unclear.…”

Osteoarthrosis

“…Although the mechanism of the enzymatic effect still remains unelucidated, it is generally assumed to be involved in bone formation. Increased activity is found in growth zones Ueffree 1969, Andersen 1970, in healing fractures (Timmer 1968), in Paget's disease (Woodard 1959), and in certain bone tumours (Jeffree and Price 1965).…”