An experimental model of ascending pyelonephritis in the rabbit

Prát, V; Hatala, M; Konícková, L; Urbanová, D

doi:10.1007/bf00960580

Cited by 7 publications

(8 citation statements)

References 5 publications

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“…These models have been developed on rats [19][20][21][22][23][24][25][26][27] . In this experiment, Balb/ C mice were used and the renal scarring model developed by intraparenchymal inoculation of LPS that had been harvested from E. coli serotype 055:B5.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Selective Cyclooxygenase-2 Inhibitor on Renal Scarring

Küçük¹,

Bingul²,

Kurt³

et al. 2006

Eur Surg Res

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Background: Scarring is one of the steps of excessive wound healing, causing dysfunction of the involved tissues and clinically poor cosmetics. The aim of this study was to examine the effect of a highly selective cyclooxygenase-2 (COX-2) inhibitor on renal scar formation in experimental pyelonephritis. Materials and Methods: Four groups of 10 Balb/C mice were formed. In groups I and II following the inoculation of lipopolysaccharide (LPS) into both kidneys, 0.18 and 0.36 mg/day of rofecoxib was given respectively via intraperitoneal route for 5 days. No medication was applied following physiological saline solution injection to both kidneys of the mice in group III (negative control group). After group IV’s LPS inoculation on the first day, saline solution (1 ml/day) was given intraperitoneally for 5 days (positive control group). Following the exposure of both kidneys, LPS of Escherichia coli (5 mg/kg) was injected into the kidneys of groups I, II, and IV. In group III, saline solution (0.1 ml) was used instead of LPS. Three days after the inoculation of LPS, solutions containing 0.18 and 0.36 mg of COX-2 inhibitor were given intraperitoneally for 5 days in groups I and II. No medication was used for the mice in group III. Six weeks after the inoculation of LPS and saline solution, all mice were humanely euthanized. Bilateral nephrectomies were done on each group of mice, and histopathological examination was performed. Results: Inoculation of LPS into the renal parenchyma caused pyelonephritis and scar formation in all groups. The degree of pyelonephritis and scar formation was lesser in groups in which COX-2 inhibitors were used. The degree of scar formation was lesser in group II, in which 0.36 mg more of COX-2 inhibitor was used than in group I (0.18 mg of COX-2 inhibitor). Conclusion: In our study model, direct inoculation of LPS to kidneys caused experimentally induced pyelonephritis. Renal scar formation was effectively prevented through the utilization of rofecoxib at 0.36-mg doses.

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Section: Discussionmentioning

confidence: 99%

Effect of a Selective Cyclooxygenase-2 Inhibitor on Renal Scarring

Küçük¹,

Bingul²,

Kurt³

et al. 2006

Eur Surg Res

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“…[77], Heptinstall [13,14] and Prat et al [20,21] were successful in producing an acute pyelonephritis.…”

Section: Discussionmentioning

confidence: 99%

“…Particularly the method of temporary ligation of the ureter and hema togenous infection with E.coli was widely adopted [13,17,20]. It is largely be lieved that infection here is caused by traumatizing the kidney (tears in the epithelium of the renal pelvis) [2,13,14,20] and by the stagnation of flow of urine [4,12.16,17,20].…”

Section: Discussionmentioning

confidence: 99%

“…It is largely be lieved that infection here is caused by traumatizing the kidney (tears in the epithelium of the renal pelvis) [2,13,14,20] and by the stagnation of flow of urine [4,12.16,17,20]. These methods of infection result in a suppurative ascending pyelonephritis followed either by death of the experimental animal or by spontaneous healing of the disease [7,13,17,20,24], A chronic pyelonephritis could only be produced in some cases. Heptinstall [14] described macroscopic and microscopic changes of chronic renal tissue inflammation in some animals lasting up to 6 months, produced by installation of bacteria and massage of the bladder.…”

Section: Discussionmentioning

confidence: 99%

“…In some tests, the kidneys remained unaffected, in others the organ was massaged or the ureter was temporarily [20,21] or permanently [16,21] ligated. Various authors succeeded [4,7,11,13,14,16,19,25] in producing an acute pyelonephritis experimentally and gained informative results.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A New Method for Producing a Chronic <i>E. coli </i>Pyelonephritis in Rabbits

L¹,

Fischer²,

Stöck³

et al. 1975

Nephron

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Chronic pyelonephritis, especially that caused by the most common invader E. coli, corresponding satisfactorily to the pathophysiology of the human disease is difficult to reproduce. We have developed a pattern of infection with which we succeeded reliably in producing a chronic E. coli pyelonephritis. This was achieved by inserting a plastic catheter into the renal pelvis. A suspension of E. coli was injected via the catheter into the renal pelvis of 32 rabbits. This pattern of infection takes a chronic progressive course and, in all cases, results in typical macroscopic and histological changes in the kidney. The bacterial excretion in the urine remains unchanged over a period of months. With the experimental procedure described, the pathogenesis and pathophysiology of chronic E. coli pyelonephritis can be clearly studied. Drugs currently used on patients can be tested for their effectiveness in long-term application.

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Pyelonephritis und Harnwegsinfekt im Experiment

Mohr¹

1977

Verhandlungsbericht Der Deutschen Gesellschaft Für Urologie

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An experimental model of ascending pyelonephritis in the rabbit

Cited by 7 publications

References 5 publications

Effect of a Selective Cyclooxygenase-2 Inhibitor on Renal Scarring

Effect of a Selective Cyclooxygenase-2 Inhibitor on Renal Scarring

A New Method for Producing a Chronic <i>E. coli </i>Pyelonephritis in Rabbits

Pyelonephritis und Harnwegsinfekt im Experiment

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