An experimental study for the development of prescription on atopic dermatitis

Abstract

Objectives : Atotang was composed of 10 kinds of traditional medicinal herb. This research was performed to examine biological effects of Atotang for the development of prescription on atopic dermatitis. Methods : Atotang was extracted with 80% EtOH. Free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total polyphenol. We observed the production of ROS, nitric oxide(NO) and the inflammatory cytokines such as interleukin-1beta(IL-β ), IL-6, tumor necrosis factor-alpha(TNF-α ), Prostaglandin E2(PGE2) in Raw 264.7 cells stimulated by LPS. We used Disc diffusion method to investigate antibacterial activity on Candida albicans, Staphylococcus aureus and Staphylococcus epidermis.Result : Content of total phenolic compound of Atotang was 36.3 mg/g ext. DPPH and ABTS scavenging activities were 77% and 46% at 200 ug/ml respectively, showing dose-dependent increase. The amounts of ROS and NO in RAW 264.7 cells were decreased by 30% and 19% at 200 ug/ml, respectively, showing dose-dependent decrease. The prodcution of IL-1beta, IL-6 and TNF-alpha in RAW 264.7 cells were decreased dose-dependently by 81%, 67%, and 20% at 200 ug/ml, respectively. Atotang was reduced LPS-stimulated production of PGE2 by 33%. Atotang on C. albicans, S. aureus and S. epidermis was selected by a disc diffusion method and inhibition effect of the Atotang on the growth of S. epidermis was the greatest. Conclusion :The results indicated that Atotang showed biological activities showing anti-oxidant, anti-inflammatory and antibacterial effects. Based on these results, it is concluded that Atotang can be applied to the prescription on atopic dermatitis.

Investigation of combined treatment of acupuncture and neurofeedback for improving cognitive function in mild neurocognitive disorder

“…The primary outcome is the Korean version of Montreal Cognitive Assessment (MoCA-K) [18] score. The secondary outcomes include Alzheimer Disease Assessment Scale–Cognitive (ADAS-Cog) [19] subscale scores, the Korean version of Beck Depression Inventory (K-BDI) [20] score, Body Awareness Questionnaire (BAQ) [21] score, breath per minute (BPM), heart rate variability (HRV) values measured during NF training, [22] functional near-infrared spectroscopy (fNIRS) [23] value, and delayed matching to sample task (DMTS) [24] score. This protocol (version 1.3, July 31, 2020) follows the Recommendations for Standard Protocol Items: Recommendations for Interventional Trial guidelines.…”