The tests on the vortex-induced vibration (VIV) of the cylinders with different helical strakes were carried out in a uniform current. Both in-line and cross-flow responses were measured and a comprehensive analysis was made in order to understand the phenomenon of VIV mitigation by helical strake and find optimal design parameters, which are more effective for VIV mitigation, of helical strakes. The results show that the cross flow response of the cylinders with helical strakes could be reduced significantly and its in-line response may be enhanced severely. Therefore, much attention should be paid to the design of helical strake.