An explicit AFLP-based marker for monitoring Fusarium oxysporum f.sp. strigae in tropical soils

Zimmermann, Judith; Klerk, M.; Musyoki, Mary K.; Viljoen, A.; Beed, Fen D.; Gorfer, Markus; Cadisch, Georg; Rasche, Frank

doi:10.1016/j.biocontrol.2015.02.008

Cited by 12 publications

(23 citation statements)

References 48 publications

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“…It was recently suggested that “Foxy-2” abundance is generally determined by physico-chemical soil properties and availability of organic N resources (Zimmermann et al, 2015), but effects of other relevant impact factors like crop growth stage and seasonality under natural field conditions are yet to be understood. It was our primary objective to address these questions through monitoring the proliferation of “Foxy-2” in the rhizosphere of crops cultivated at two agro-ecologically contrasting field sites in western Kenya considering three plant growth stages throughout two cropping seasons.…”

Section: Discussionmentioning

confidence: 99%

“…Quantification of “Foxy-2” gene copy numbers in soils was performed using Fos-specific oligonucleotides Kb1 (5′-GGACGAACTGACAGCCCTAC-3′) and Kb2 (5′-GTAACCGTAATATTGTTCAGAGCTC-3′) (Zimmermann et al, 2015). Each reaction (20 μl) contained 10 ng rhizosphere soil DNA template, 10 μl of Power SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA), 0.2 µM of each oligonucleotide Kb1 and Kb2, as well as 0.2 μl T4 gene 32 protein (500 ng μl −1 , MP Biomedicals).…”

Section: Methodsmentioning

confidence: 99%

“…Concentration and quality of “Foxy-2” DNA were determined as described above. Five ng of “Foxy-2” DNA was used as template #1 for Fos-specific qPCR (using oligonucleotides Kb1:Kb2 with the protocol published in Zimmermann et al (2015)) and template #2 for 18S rDNA qPCR (see above). The 5 ng “Foxy-2” DNA template used for both qPCR assays corresponded to 2.3*10 5 “Foxy-2” gene copies and 4.6*10 5 18S rDNA gene copies resulting in a ratio of 1:2 between “Foxy-2” and 18S rDNA gene copies.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, a thorough understanding of environmental conditions which promote the proliferation and persistence of the BCA “Foxy-2” is required to ensure consistent and sustained S. hermonthica control. In this context, the monitoring of “Foxy-2” proliferation under contrasting soil conditions was issued by Zimmermann et al (2015) who, using a Fos specific and quantitative monitoring tool, confirmed under controlled conditions that “Foxy-2” proliferation was determined by soil texture and promoted by the amendment of nitrogen (N)-rich organic resources. The latter fact requires particular attention since “Foxy-2” is a soil borne fungus and proliferates saprophytically and endophytically in crop rhizospheres and roots, respectively, where it has to compete with indigenous microorganisms for organic resources (Ndambi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies

et al. 2016

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Our objectives were to (1) monitor the proliferation of the biocontrol agent (BCA) Fusarium oxysporum f. sp. strigae strain “Foxy-2”, an effective soil-borne BCA against the parasitic weed Striga hermonthica, in the rhizosphere of maize under different agro-ecologies, and (2) investigate its impact on indigenous rhizosphere fungal community abundance and composition. Field experiments were conducted in Busia and Homa Bay districts in western Kenya during two cropping seasons to account for effects of soil type, climate, growth stage and seasonality. Maize seeds were coated with or without “Foxy-2” and soils were artificially infested with S. hermonthica seeds. One treatment with nitrogen rich organic residues (Tithonia diversifolia) was established to compensate hypothesized resource competition between “Foxy-2” and the indigenous fungal community. Rhizosphere soil samples collected at three growth stages (i.e., EC30, EC60, EC90) of maize were subjected to abundance measurement of “Foxy-2” and total indigenous fungi using quantitative polymerase chain reaction (qPCR) analysis. Terminal restriction fragment length polymorphism (TRFLP) analysis was used to assess potential alterations in the fungal community composition in response to “Foxy-2” presence. “Foxy-2” proliferated stronger in the soils with a sandy clay texture (Busia) than in those with a loamy sand texture (Homa Bay) and revealed slightly higher abundance in the second season. “Foxy-2” had, however, only a transient suppressive effect on total indigenous fungal abundance which ceased in the second season and was further markedly compensated after addition of T. diversifolia residues. Likewise, community structure of the indigenous fungal community was mainly altered by maize growth stages, but not by “Foxy-2”. In conclusion, no adverse effects of “Foxy-2” inoculation on indigenous fungal rhizosphere communities were observed corroborating the safety of this BCA under the given agro-ecologies.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies

et al. 2016

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“…Genomic fingerprinting techniques have been widely used to study genetic variability, population structure, and species phylogeny of F. oxysporum (Silva et al, 2013;Chen et al, 2014;Zimmermann et al, 2015). Among the techniques, amplified fragment length polymorphism (AFLP) is well recognized which has great discriminatory power (Vos et al, 1995;Mueller & Wolfenbarger, 1999;Silva et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and Genetic Diversity of Fusarium oxysporum Causing Soybean Root Rot in Northeast China

Li¹,

Zhao²,

Hua³

et al. 2018

JAS

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Soybean is an important edible legume cultivated around the world. However, soybean production is seriously impacted by the widespread of root rot disease. In this study, genetic diversity and pathogenicity of Fusarium oxysporum associated with root rot of soybean in Heilongjiang province, China, were examined. A total of 50 F. oxysporum strains were isolated from diseased soybean plants grown in Harbin, Heihe, Jixi, Jiamusi and Qiqihar of Heilongjiang province. Pathogenicity study indicated that all F. oxysporum strains were able to induce root rot disease on soybean in which 28% of the isolates were highly aggressive, 42% were moderately aggressive, and 30% were weakly aggressive. Aggressiveness of the isolates did not appear to be associated with geographic location or plant age of isolation. Genomic DNA of the isolates was analyzed by polymerase chain reaction using eight amplified fragment length polymorphism (AFLP) primers that generated 1728 bands, of which 99% were polymorphic. Cluster analysis using UPGMA showed that the similarity values ranged from 0.15 to 0.47. At a similarity coefficient of 0.2, the isolates were separated into 7 groups. Analysis of molecular variance indicated that about 92% of the genetic variation resided within populations. No correlation was found between genetic diversity and aggressiveness or the geographic origin of the isolates. Results of the study indicate that pathogenic F. oxysporum are commonly associated with root rot of soybean with various aggressiveness and they are genetically diverse.

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Development of Mycoherbicides

Berestetskiy

2021

Encyclopedia of Mycology

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An explicit AFLP-based marker for monitoring Fusarium oxysporum f.sp. strigae in tropical soils

Cited by 12 publications

References 48 publications

Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies

Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies

Pathogenicity and Genetic Diversity of Fusarium oxysporum Causing Soybean Root Rot in Northeast China

Development of Mycoherbicides

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