An extended map of the sugar beet genome containing RFLP and RAPD loci

Barzen, E.; Mechelke, W.; Ritter, Enrique; Schulte-Kappert, E.; Salamini, F.

doi:10.1007/bf00222201

Cited by 89 publications

(42 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genetic diversity of sugar beet has been ascertained in past using morphological traits, isozymes and molecular marker systems. DNA marker systems such as RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism) were used in sugar beet in early and mid-90s (Jung et al, 1993;Barzen et al, 1995;Schondelmaier et al, 1996). Linkage maps and QTL mapping based on RAPD, AFLP, SNP (Single Nucleotide Polymorphisms) and microsatellites / SSR (Simple Sequence Repeat) markers were also attempted (Möhring et al, 2005;Grimmer et al, 2007;Rae et al, 2000;Schneider et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of sugar beet genotypes evaluated by microsatellite DNA markers

Srivastava¹,

Pathak²,

Kumar³

et al. 2017

JEB

View full text Add to dashboard Cite

Aim: Methodology:Results: Interpretation:Identification of diverse sugar beet (Beta vulgaris L.) genotypes is essential for using them as parents in sugar beet improvement programme of India. Hence, genetic variation and diversity of elite sugar beet germplasm collection was analysed using microsatellite markers.Genomic DNA of thirteen genotypes was amplified using 14 microsatellite primers containing dinucleotide to compound repeat motifs. Amplified bands were scored on gel, transformed into a binary character matrix and analyzed through NTSYS software for clustering in heterotic groups.A total of 243 amplicons were resolved and grouped into 88 alleles of distinct molecular weight ranging from 124 to 1222 bp with an average of 17.36 amplicons/primer, 4 to10 alleles/SSR locus and moderate to high PIC ranging from 0.625-0.851. Similarity coefficients ( S M ) b a s e d o n t h e presence/absence of alleles ranged from 0.47 to 0.89 (mean value of 0.65). UPGMA dendrogram based on SM, grouped these genotypes in two major taxonomical groups with five clusters having one, four, three, three and two genotypes each. Clustering pattern of UPGMA matched with the 2D and 3D scatter plots of MDS and PCA. The highly significant cophenetic coefficient of r = 0.96 proved that these SSR markers efficiently analyzed the genetic relationships of sugar beet genotypes.Microsatellite markers can be used as a potential cost effective method for exploring molecular genetic diversity in sugar beet in order to obtain new genetic combinations.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic diversity of sugar beet genotypes evaluated by microsatellite DNA markers

Srivastava¹,

Pathak²,

Kumar³

et al. 2017

JEB

View full text Add to dashboard Cite

show abstract

“…Currently, they mainly consist of dense molecular linkage maps based on RFLP, RAPD and AFLP markers, to which several expressed genes and few mutants have been associated ͑Barzen et al 1992; Barzen et al 1995;Schumacher et al 1997;Schneider et al 1999;Schneider et al 2002;Hunger et al 2003͒. Additionally, large insert libraries of YAC and BAC clones are available, and positional cloning was implemented to identify a gene conferring resistance to nematodes ͑Cai et al 1997;Hohmann et al 2003͒.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed, linkage group-specific SNP marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.)

M�hring¹,

Salamini²,

Schneider³

2004

Molecular Breeding

View full text Add to dashboard Cite

A set of single nucleotide polymorphism ͑SNP͒ markers has been developed for each of the nine linkage groups of sugar beet. Each set can monitor the polymorphic state at five to six linked marker loci. In each set, the loci selected for marker development are first amplified in a multiplexed reaction. These amplification products are the basis for sequence-specific elongation of primers adjacent to SNP positions. The extension step revealing SNP loci is based on fluorescently labelled nucleotides. In each set, primers developed to reveal SNP alleles differ in length to allow clear peak resolution in capillary electrophoresis. The nine linkage group ͑LG͒ Ϫ specific sets provide information on the polymorphism at a total of 52 SNP marker loci. Using the SNP-based tool, groups of concerned loci have been anchored to three different linkage maps of sugar beet. In a second experiment, sugar beet breeding lines have been fingerprinted. The use of the nine sets of LG-specific markers in sugar beet genetics and breeding is discussed. The information necessary to specify the 52 marker loci, as well as their map location, and all details concerning SNP assays, including allele type and nature of mutation, are reported. Abbreviations:LG -linkage group; SNP -single nucleotide polymorphism; RFLP -restriction fragment length polymorphism; RAPD -random amplification of polymorphic DNA; AFLP -amplified fragment length polymorphism; CAPS -cleaved amplified polymorphic sequence; SSCP -single strand conformation polymorphism; SSR -simple sequence repeat; STS -sequence tagged site; QTL -quantitative trait locus; nt -nucleotide

show abstract

“…The method has been used to study the major genes of four morphological traits mapped on an RFLP map:annual habit (Boudry et al, 1994), rhizomania resistance, monogermity, and hypocotyl color (Barzen et al, 1992(Barzen et al, , 1995. The monogerm or multigerm gene M-m has been mapped on the fifth linkage group and is closely linked with the makers 7, 49, 117, and 238a (Barzen et al, 1995). In our experiments, we used the RFLP probe 49 (Barzen et al, 1995).…”

mentioning

confidence: 99%

“…The monogerm or multigerm gene M-m has been mapped on the fifth linkage group and is closely linked with the makers 7, 49, 117, and 238a (Barzen et al, 1995). In our experiments, we used the RFLP probe 49 (Barzen et al, 1995). The aim of the work was to study RFLP in Japanese breeding lines TK-80-2BR 2 mm-CMS and TK-80-2BR 2 mm-O, both with incomplete monogermity.…”

mentioning

confidence: 99%

Analysis of restriction fragment length polymorphism of Japanese sugar beet lines with incomplete monogermity.

1997

View full text Add to dashboard Cite

show abstract

An extended map of the sugar beet genome containing RFLP and RAPD loci

Cited by 89 publications

References 23 publications

Genetic diversity of sugar beet genotypes evaluated by microsatellite DNA markers

Genetic diversity of sugar beet genotypes evaluated by microsatellite DNA markers

Multiplexed, linkage group-specific SNP marker sets for rapid genetic mapping and fingerprinting of sugar beet (Beta vulgaris L.)

Analysis of restriction fragment length polymorphism of Japanese sugar beet lines with incomplete monogermity.

Contact Info

Product

Resources

About