An extraction method for measuring soil microbial biomass C

Vance, Eric D.; Brookes, Philip C.; Jenkinson, D. S.

doi:10.1016/0038-0717(87)90052-6

Cited by 10,436 publications

(4,792 citation statements)

References 17 publications

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“…After the last gas exchange measurements, the hand-sorted and well mixed soil was sampled and analyzed for NH + 4 , NO − 3 , dissolved organic nitrogen (DON), dissolved organic carbon (DOC), microbial biomass carbon (C MIC ), and microbial biomass nitrogen (N MIC ) following standard extraction and fumigation-extraction procedures (Jenkinson and Powlson, 1976; Vance et al, 1987; Rinnan et al, 2008). …”

Section: Methodsmentioning

confidence: 99%

Off-season biogenic volatile organic compound emissions from heath mesocosms: responses to vegetation cutting

et al. 2013

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Biogenic volatile organic compounds (BVOCs) affect both atmospheric processes and ecological interactions. Our primary aim was to differentiate between BVOC emissions from above- and belowground plant parts and heath soil outside the growing season. The second aim was to assess emissions from herbivory, mimicked by cutting the plants. Mesocosms from a temperate Deschampsia flexuosa-dominated heath ecosystem and a subarctic mixed heath ecosystem were either left intact, the aboveground vegetation was cut, or all plant parts (including roots) were removed. For 3–5 weeks, BVOC emissions were measured in growth chambers by an enclosure method using gas chromatography-mass spectrometry. CO2 exchange, soil microbial biomass, and soil carbon and nitrogen concentrations were also analyzed. Vegetation cutting increased BVOC emissions by more than 20-fold, and the induced compounds were mainly eight-carbon compounds and sesquiterpenes. In the Deschampsia heath, the overall low BVOC emissions originated mainly from soil. In the mixed heath, root, and soil emissions were negligible. Net BVOC emissions from roots and soil of these well-drained heaths do not significantly contribute to ecosystem emissions, at least outside the growing season. If insect outbreaks become more frequent with climate change, ecosystem BVOC emissions will periodically increase due to herbivory.

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Section: Methodsmentioning

confidence: 99%

Off-season biogenic volatile organic compound emissions from heath mesocosms: responses to vegetation cutting

et al. 2013

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“…Soil TN and SOC were determined using a CN Analyzer (Vario Max, Elementar, Germany). MBC was measured using the chloroform fumigation and direct extraction technique (Vance et al, 1987). A conversion factor of 2.64 was used to convert extracted C to biomass C.…”

Section: Soil Sampling and Analysismentioning

confidence: 99%

Responses of absolute and specific soil enzyme activities to long term additions of organic and mineral fertilizer

Zhang

Dong

Dai

et al. 2015

Science of The Total Environment

171

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• Acid phosphatase activity was negatively correlated with soil pH and P content.• βG, NAG, and LAP activities were positively correlated with SOC and Total N contents.• The four enzyme activities were significantly higher under manure than NPK fertilizer.• Enzyme activities were positively correlated with actinomycete and G + bacterium.• We recommend reducing P fertilizer application rates to subtropical paddy soils. G R A P H I C A L A B S T R A C Ta b s t r a c t a r t i c l e i n f o Long-term phosphorus (P) and nitrogen (N) applications may seriously affect soil microbial activity. A long-term field fertilizer application trial was established on reddish paddy soils in the subtropical region of southern China in 1998. We assessed the effects of swine manure and seven different rates or ratios of NPK fertilizer treatments on (1) the absolute and specific enzyme activities per unit of soil organic carbon (SOC) or microbial biomass carbon (MBC) involved in C, N, and P transformations and (2) their relationships with soil environmental factors and soil microbial community structures. The results showed that manure applications led to increases in the absolute and specific activities of soil β-1,4-glucosidase(βG), β-1,4-N-acetylglucosaminidase (NAG), and leucine aminopeptidase (LAP). The absolute and specific acid phosphatase (AP) activities decreased as mineral P fertilizer application rates and ratios increased. Redundancy analysis (RDA) showed that there were negative correlations between absolute and specific AP activities, pH, and total P contents, while there were positive correlations between soil absolute and specific βG, NAG, and LAP enzyme activities, and SOC and total N contents. RDA showed that the contents of actinomycete and Gram-positive bacterium PLFA biomarkers are more closely related to the absolute and specific enzyme activities than the other PLFA biomarkers (P b 0.01). Our results suggest that both the absolute and specific enzyme activities could be used as sensitive soil quality indicators that provide useful linkages with the microbial community structures and environmental factors. To maintain microbial activity and to minimize environmental impacts, P should be applied as a combination of inorganic and organic forms, and total P fertilizer application rates to subtropical paddy soils should not exceed 44 kg P ha −1 year −1 .

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“…The fumigation-extraction method (Vance et al, 1987) was used to determine the soil microbial biomass C of the soil samples. The content of K 2 SO 4 -extracted C from the CHCl 3 -treated and untreated soils was determined by an automated TOC analyzer.…”

Section: Microbial Biomassmentioning

confidence: 99%

Structure and function of rhizosphere and non-rhizosphere soil microbial community respond differently to elevated ozone in field-planted wheat

Chen¹,

Wang²,

He³

2015

Journal of Environmental Sciences

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An extraction method for measuring soil microbial biomass C

Cited by 10,436 publications

References 17 publications

Off-season biogenic volatile organic compound emissions from heath mesocosms: responses to vegetation cutting

Off-season biogenic volatile organic compound emissions from heath mesocosms: responses to vegetation cutting

Responses of absolute and specific soil enzyme activities to long term additions of organic and mineral fertilizer

Structure and function of rhizosphere and non-rhizosphere soil microbial community respond differently to elevated ozone in field-planted wheat

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